Summary
600,000 seedlings ofAlnus crispa were inoculated with a 1∶1∶1 mixture of theFrankia strains ACN1AG, AGN1 AGexo and MGP10i. After 3 successive inoculations and screenings, one individual, AC-4, was selected as non-nodulating (Nod−) with Frankiae. This selected individual AC-4 (Nod−) and two other clones ofA. crispa, AC-2 and AC-5, known for their ability to nodulate (Nod+) and two other clones ofA. crispa, AC-2 and AC-5, known for their ability to nodulate (Nod+) withFrankia werein vitro propagated. The different clones ofA. crispa in culture required different kinds and concentrations of sugar during the in vitro multiplication and rooting stages. Nodulation tests using 7Frankia strains indicated that the clone AC-4 (Nod−) was non-nodulating with 6 of the 7Frankia strains tested. One strain,Frankia ANNI, isolated from one unique nodule produced on the mother-plant AC-4, induced 38% of the AC-4 plantlets to nodulate but with a number of nodules 10 to 20 times less than the clones AC-2 (Nod+) and AC-5 (Nod+). Morphological observations of the roots of AC-4 (Nod−) indicated that this clone had few and abnormally short root hairs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akkermans A D L and Houwers A 1979 Symbiotic nitrogen-fixers for use in temperate forestry.In Symbiotic Nitrogen Fixation in the Management of temperature Forests. Eds. J C Gordon, C T Wheeler and D A Perry. Oregon State Univ., Corvallis, OR, pp 23–37.
Berry A and Torrey J G 1979 Isolation and characterizationin vivo andin vitro of an actinomycetous endophyte fromAlnus rubra Bong.In Symbiotic Nitrogen Fixation in the Management of temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry. Oregon State University, Corvallis, OR, pp 69–83.
Dawson J O and Sun S H 1981 The effect ofFrankia isolates fromComptonia peregrina andAlnus crispa on the growth ofAlnus glutinosa, A. cordata andA. incana clones. Can. J. For. Res. 11, 758–762.
Garton S, Hosier N A, Read P E, and Farnham R S 1981In vitro propagation onAlnus glutinosa Gaertn. Hortic. Sci. 16, 758–759.
Hall R B and Maynard C A 1979 Considerations in the genetic improvement of alder.In Symbiotic Nitrogen Fixation in the Management of temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry. Oregon State University, Corvallis, OR. pp 322–344.
Jones OP, Hopgood M E and O'Farrell D 1977 Propagationin vitro of M26 apple rootstocks. J. Hortic. Sci. 52, 235–238.
Koch B and Evans H J 1966 Reduction of acetylene to ethylene by soybean root nodules. Plant Physiol. 41, 1748–1750.
Lalonde M 1977 Infection process of theAlnus root nodule symbiosis.In Recent Developments in Nitrogen Fixation. Eds. W E Newton, J R Postgate and C Rodriguez-Barrueco. Ac. Press, London. pp 569–589.
Lalonde M 1979 A simple and rapid method for the isolation, cultivationin vitro and characterization ofFrankia strains fromAlnus root nodules.In Symbiotic Nitrogen Fixation in the Management of temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry. Oregon State University, Corvallis, OR., p 480.
Lalonde M 1979 Techniques and observations of the nitrogen-fixingAlnus root nodules symbiosis.In Recent Advances in biological Nitrogen Fixation. Ed. N.S. Subba Rao, Oxford & IBH Publ., New Delhi. pp 421–434.
Lalonde M and Calvert H E 1979 Production ofFrankia hyphae and spores as an infective inoculant forAlnus species.In Symbiotic Nitrogen Fixation in the Management of temperate Forests. Eds. J C Gordon, C T Wheeler and D A Perry. Oregon State University, Corvallis, OR, pp 95–110.
Lalonde M, Calvert H E and Pine S 1981 Isolation and use ofFrankia strains in actinorhizae formation.In Current Perspectives in Nitrogen Fixation. Eds. A H Gibson and W E Newton. Australian Academy of Science, Canberra. pp 296–299.
Mort A, Normand P and Lalonde M 1983 2-O-Methyl-D-mannose, a key sugar in the taxonomy ofFrankia. Can. J. Microbiol. 29, 00–00.
Murashige T and Skoog F 1962 A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15, 473–497.
Nambiar P T C, Nigam S N, Dart P J and Gibbons R W 1983 Absence of root hairs in non-nodulating groundnut,Arachis hypogeae L. J. Exp. Bot. 34, 484–488.
Normand P, and Lalonde M 1982 Evaluation ofFrankia strains isolated from provenances of twoAlnus species. Can. J. Microbiol. 28, 1133–1142.
Perinet P and Lalonde M 1983In vitro propagation and nodulation of the actinorhizal host plantAlnus glutinosa (L.) Gaertn. Plant Sci. Lett. 29, 9–17.
Read P E, Garton S, Louis K and Zimmerman E S 1982In vitro propagation of species for bioenergy plantations.In Proc. 5th Intern. Cong. Plant Tissue and Cell Culture. Ed. Akio Fujiwara. pp 757–758.
Torrey J G and Callaham D 1979 Early nodule development inMyrica gale. Bot. Gaz. 140, (S), S10-S14.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tremblay, F.M., Nesme, X. & Lalonde, M. Selection and micropropagation of nodulating and non-nodulating clones ofAlnus crispa (Ait.) Pursh. Plant Soil 78, 171–179 (1984). https://doi.org/10.1007/BF02277848
Issue Date:
DOI: https://doi.org/10.1007/BF02277848