Summary
The role of fimbriae in enterobacterial adhesion to roots of grasses and cereals is discussed. All nitrogen-fixing enteric bacteria isolated in Finland had fimbriae. AllEnterobacter isolates had mannose-binding type-1 fimbriae, whereas most of theKlebsiella isolates had both type-1 and type-3 fimbriae. The strains were isolated from a total of ten different grass species, and no specific association was found between grass species and bacterial fimbriation, biogroup or serogroup. Purified, radiolabeled fimbriae bound to roots ofPoa pratensis in vitro, and bacterial adhesion was inhibited by Fab fragments specific for fimbriae.Klebsiella strains carrying type-3 fimbriae adhered to roots of various grasses and cereals more efficiently than type-1- or nonfimbriated strains, and it was concluded that type-3 fimbriae are the major adhesions ofKlebsiella. Immunofluorescence studies revealed that the bacteria preferentially adhered to root hairs, and to a lesser extent, to the zone of elongation and the root cap mucilage. No strict host specificity in enterobacterial adhesion was observed.
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Barak R, Nur I and Okon Y 1983 Detection of chemotaxis inAzospirillum brasilense. J. Appl. Bacteriol. 53, 399–403.
Dazzo F B 1981 Bacterial attachment as related to cellular recognition in theRhizobium-legume symbiosis. J. Supramol. Struct. Cell. Biochem. 16, 29–41.
Duguid J P 1959 Fimbriae and adhesive properties inKlebsiella strains. J. Gen. Microbiol. 21, 271–286.
Duguid J P and Old D C 1980 Adhesive properties ofEnterobacteriaceae, pp 185–217.In Bacterial Adherence, Receptors and Recognition. Ed. E H Beachey. Series B, Vol 6, Chapman and Hall, London.
Ewing W H and Fife M A 1972Enterobacter agglomerans (Beijerinck) comb. nov. (the Herbicola-Lathyri bacteria). Int. J. Syst. Bacteriol. 22, 4–11.
Gaastra W and de Graaf F K 1982 Host-specific fimbrial adhesins of noninvasive enterotoxigenicEscherichia coli strains. Microbiol. Rev. 46, 129–161.
Haahtela K and Korhonen T K 1984In vitro adhesion of N2-fixing enteric bacteria to roots of grasses and cereals. Appl. Environm. Microbiol. 49, 1186–1190.
Haahtela K, Tarkka E and Korhonen T K 1984 Type-1-fimbriae-mediated adhesion of enteric bacteria to grass roots. Appl. Environm. Microbiol. 49, 1182–1185.
Hrabak E M, Urbano M A and Dazzo F B 1981 Growth-phase-dependent immunodeterminants ofRhizobium trifolii lipopolysaccharide which binds Trifoliin A, a white clover lectin. J. Bacteriol. 148, 697–711.
Kijne J W, van der Schaal C A M, Diaz C L and van Iren F 1983 Mannose-specific lectins and the recognition of pea roots byRhizobium leguminosarum. pp 521–530,In Lectins, Vol 3. Eds T C Bog-Hansen and G A Spleger. W de Gruyter and Co, Berlin-New York.
Korhonen T K 1979 Yeast cell agglutination by purified enterobacterial pili. FEMS Microbiol. Lett. 6, 421–425.
Korhonen T K, Tarkka E, Ranta H and Haahtela K 1983 Type 3 fimbriae ofKlebsiella sp: Molecular characterization and role in bacterial adhesion to plant roots. J. Bacteriol. 155, 860–865.
Korhonen T K, Väisänen-Rhen V, Rhen M, Pere A, Parkkinen J and Finne J 1984Escherichia coli fimbriae recognizing sialyl galactosides. J. Bacteriol. 159, 762–766.
Korhonen T K, Väisänen V, Saxen H, Hultberg H and Svenson S B 1982 P-antigen-recognizing fimbriae from human uropathogenicEscherichia coli strains. Infect. Immun. 37, 286–291.
Lippincot B B, Whatley M H and Lippincot J A 1977 Tumor induction byAgrobacterium involves attachment of the bacterium to a site on the host plant cell wall. Plant Physiol. 59, 388–390.
Nowicki B, Rhen M, Väisänen-Rhen V, Pere A and Korhonen T K 1984 Immunofluorescence study of fimbrial phase variation inEscherichia coli KS71. J. Bacteriol. 160, 691–695.
Ofek I, Mirelman D and Sharon N 1977 Adherence ofEscherichia coli to human mucosal cells mediated by mannose receptors. Nature (London) 265, 623–625.
Old D C 1972 Inhibition of the interaction between fimbrial hemagglutinins and erythrocytes by D-mannose and other carbohydrates. J. Gen. Microbiol. 71, 149–157.
Old D C and Adegbola R A 1984 A comparative immunoelectronmicroscopical study of fimbriae ofEnterobacter andKlebsiella. System. Appl. Microbiol. 5, 157–168.
Patriquin DG, Döbereiner J and Jain D K 1983 Sites and processes of association between diazotrophs and grasses. Can. J. Microbiol. 29, 900–915.
Rhen M, Klemm P, Wahlström E, Svenson S B, Källenius G and Korhonen T K 1983 P-fimbriae ofEscherichia coli: immuno- and protein-chemical characterization of fimbriae from two pyelonephritogenic strains. FEMS Microbiol. Lett. 18, 233–238.
Rhen M, Knowles J, Penttilä M E, Sarvas M and Korhonen T K 1983 P fimbriae ofEscherichia coli: molecular cloning of DNA fragments containing the structural genes. FEMS Microbiol. Lett. 19, 119–123.
Rhen M, Mäkelä P H and Korhonen T K 1983 P fimbriae ofEscherichia coli are subject to phase variation. FEMS Microbiol. Lett. 19, 267–271.
Umali-Garcia M, Hubbel D H, Gaskins M H and Dazzo F B 1980 Association ofAzospirillum with grass roots. Appl. Environ. Microbiol. 39, 219–226.
Vesper S J and Bauer W D 1984 Role of pili inRhizobium attachment. Proceedings of the Second International Symposium on the Molecular Genetics of Bacteria-Plant Interaction, Abstract No 99, Ithaca, USA.
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Korhonen, T.K., Nurmiaho-Lassila, EL., Laakso, T. et al. Adhesion of fimbriated nitrogen-fixing enteric bacteria to roots of grasses and cereals. Plant Soil 90, 59–69 (1986). https://doi.org/10.1007/BF02277387
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DOI: https://doi.org/10.1007/BF02277387