Summary
InRhizobium lupini bacterioids enzymes catalysing biosynthesis of aspartic acid have been found. The first enzyme termed aspartate dehydrogenase catalyses synthesis of aspartate from oxaloacetic acid and ammonia in the presence of NADH. The second enzyme, aspartase (L-aspartate ammonialyase, EC 4.3.1.1.), catalyses synthesis of aspartate from fumaric acid and ammonia. These data show that ammonia can be assimilated not only in the plant part of nodules but also in bacteroids. Biosynthesis of aspartate plays a very important role in the assimilation of ammonia in nodules.
Similar content being viewed by others
References
Batt, T. and Brown, D. H. 1974 The influence of inorganic nitrogen supply on amination and related reactions in the blue-green alga,Anabaena cylindrica Lemm. Planta Berlin116, 27–37.
Davis, B. J. 1964 Disc electrophoresis. Method and application to human serum proteins. Ann. N.Y. Acad. Sci.121, 404–427.
Depue, R. H. and Moat, A. G. 1961 Factors affecting aspartase activity. J. Bacteriol.82, 383–386.
Ehkesmann, B., Imbault, J. H. and Well, J. H. 1973 Spectrophotometric determination of protein concentration in cell extracts containing tRHA's and rRNA's. Anal. Biochem.54, 454–463.
Emery, T. F. 1963 Aspartase-catalyzed synthesis of N-hydroxy aspartic acid. Biochemistry2, 1041–1045.
Fujihara, S. and Yamaguchi, M. 1980 Asparagine formation in soybean nodules. Plant Physiol.66, 139–141.
Gordon, A. H. and Lois, L. W. 1967 Preparative acrylamide electrophoresis. A simple gel system. Anal. Biochem.21, 190–197.
Kretovich, W. L., Bronovitskaja, Z. S., and Kariakina, T. I. 1963 The reductive amination of pyruvic, oxaloacetic and hydroxypyruvic acids in plants. Dokl. Akad. Nauk SSSR, Ser. Biol.152, 1247–1249.
Kretovich, W. L., Kariakina, T. I., Charahchjan, W. W., Kaloshina, G. S., Sidelnikova, L. I. and Shaposhnikov, G. L. 1978 Biosynthesis of aspartate from oxaloacetate and ammonia in plants. Dokl. Akad. Nauk SSSR, Ser. Biol.243, 793–796.
Kovacheva, N. G. 1978 Glutamate and aspartate dehydrogenase activities inAnabaena variabilis Kütz. Plant Physiol. (Bulgarian Acad. Sci.) IV,3, 54–64.
Lea, P. J. and Fowden, L. 1975 The purification and properties of glutamine-dependent asparagine synthetase isolated from Lupinus albus. Proc. R. Soc. London.192B, 13–26.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. 1951 Protein measurement with the Folin phenol reagent. J. Biol. Chem.193, 265–275.
Pate, J. S., Gunning, B. E. S. and Briarty, L. G. 1969 Ultrastructure and functioning of the transport system of the leguminous root nodule. Planta Berlin85, 11–34.
Radyukina, N. A., Pushkin, A. V., Evstigneeva, Z. G. and Kretovich, W. L. 1977 Ammonia assimilation in lupin nodules during its vegetation. Dokl. Akad. Nauk SSSR234, 1209–1211.
Robertson, J. G., Warburton, M. P. and Farnden, K. J. F. 1975 Induction of glutamate synthase during nodule development in lupin. FEBS Lett.55, 33–37.
Rognes, S. E. 1975 Glutamine-dependent asparagine synthetase fromLupinus luteus. Phytochemistry14, 1975–1982.
Rowell, P. and Stewart, W. D. P. 1976 Alanine dehydrogenase of the N2-fixing blue-green algaAnabaena cylindrica. Arch. Microbiol.107, 115–124.
Scott, D. B., Farnden, K. J. F. and Robertson, J. G. 1976 Ammonia assimilation in lupin nodules. Nature London263, 703–705.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kretovich, W.L., Kariakina, T.I., Weinova, M.K. et al. The synthesis of aspartic acid inRhizobium lupini bacteroids. Plant Soil 61, 145–156 (1981). https://doi.org/10.1007/BF02277371
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02277371