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Non-radioactive determination of PTH and dabsyl phosphoamino acids by capillary electrophoresis

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Summary

Capillary electrophoresis is a novel technique in the non-radioactive determination of phosphoamino acids. The main advantage of the method presented is the high selectivity and the ability to separate all phosphoamino acid derivatives. Non-radioactive determination of PTH or dabsyl phosphoamino acids by capillary electrophoresis provides a fast and simple screening procedure for all O-phosphorylated amino acids in protein and peptides in the low picomolar range.

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References

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Abteilung Biochemie Supramolekularer Systeme, Protein-Sequenzlabor, MA 2/143, Ruhr-Universität Bochum, 4630, Bochum, Germany

    M. Heber, Chr Liedtke, H. Korte, E. Hoffmann-Posorske & H. E. Meyer

  2. Dipartimento di Chimica Biologica, University di Padova, Via Trieste 75, 35121, Padova, Italy

    A. Donella-Deana & L. A. Pinna

  3. Department of Organic Chemistry, University of Melbourne, 3052, Parkville, Victoria, Australia

    J. Perich, E. Kitas & R. B. Johns

Authors
  1. M. Heber
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  2. Chr Liedtke
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  3. H. Korte
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  4. E. Hoffmann-Posorske
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  5. A. Donella-Deana
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  6. L. A. Pinna
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  7. J. Perich
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  8. E. Kitas
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  9. R. B. Johns
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  10. H. E. Meyer
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Heber, M., Liedtke, C., Korte, H. et al. Non-radioactive determination of PTH and dabsyl phosphoamino acids by capillary electrophoresis. Chromatographia 33, 347–350 (1992). https://doi.org/10.1007/BF02275916

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  • DOI: https://doi.org/10.1007/BF02275916

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