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Autoradiographical and immunohistochemical analysis of receptor localization in the central nervous system

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Summary

Quantitative receptor autoradiographic methods have been widely used over the past two decases. Some of the advantages and limitations of these techniques are reviewed here. Comparison with immunohistochemical andin situ hybridization methods is also highlighted, as well as the use of these approaches to study receptor gene over-expression in cell lines. Together, data obtained using these various methodologies can provide unique information on the potential physiological roles of a given receptor protein and/or binding sites in various tissues.

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  1. Douglas Hospital Research Centre and Department of Psychiatry, Faculty of Medicine, McGill University, 6875 La Salle Boulevard, H4H 1R3, Verdun, Quebec, Canada

    Jean-Guy Chabot, Satyabrata Kar & Rémi Quirion

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Chabot, JG., Kar, S. & Quirion, R. Autoradiographical and immunohistochemical analysis of receptor localization in the central nervous system. Histochem J 28, 729–745 (1996). https://doi.org/10.1007/BF02272147

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