Abstract
A bacterial disease occurred on fruiting bodies ofLentinula edodes that formed outdoors onQuercus bedlogs during winter. The pathogen was identified asPseudomonas tolaasii based on morphological and bacteriological characteristics. Symptoms exhibited by infected fruiting bodies ranged from mild browning to severe necrotic cavities that characteristically developed in the cap tissue along the periphery of the attachment area to the stalk. The mode of symptom development was greatly influenced by the internal tissue structure of fruiting bodies. Multiplication of bacterial cells within the fruiting bodies was strictly intercellular and thus differed from previously reported bacterial disease ofL. edodes incited by an unidentified rod-shaped bacterium. The present strain ofP. tolaasii was capable of attacking theL. edodes mycelium in the inner bark and outer sapwood regions and caused lysis of heavily infected hyphae.
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Literature cited
De la Cruz, J., Pintor-Toro, J. A., Benitez, T. and Llobell, A. 1995. Purification and characterization of an endo-β-1,6-glucanase fromTrichoderma harzianum that is related to its mycoparasitism. J. Bacteriol.177: 1864–1871.
Fermor, T. R. 1987. Bacterial diseases of edible mushrooms and their control. In: “Cultivating edible fungi,” (ed. by Wuest, P. J., Royse, D. J. and Beelman, R. B.), pp. 361–370. Elsevier, Amsterdam.
Fletcher, J. T., White, P. F. and Gaze, R. H. 1989. “Mushrooms: pest and disease control, 2nd ed.,” Intercept, Ponteland. 174p.
Komatsu, M. and Goto, M. 1974. Bacterial disease of cultivated shiitake-mushroom,Lentinus edodes (Berk.) Sing. in Japan. Rept. Tottori Mycol. Inst.11: 69–82. (In Japanese.)
Lelliott, R. A., Billing, E. and Hayward, A. C. 1966. A determinative scheme for the fluorescent plant pathogenic pseudomonads. J. Appl. Bacteriol.29: 470–489.
Nakai, Y., Ushiyama, R. and Komatsu, M. 1982. Presence of a rod-shaped bacterium inLentinus edodes fruit-bodies with a browning symptom. Rept. Tottori Mycol. Inst.20: 47–53.
Nishiyama, K. 1978. A proposal of a simple identification method for plant pathogenic bacteria. Plant Protection (Shokubutu Bôeki)32: 283–288. (In Japanese.)
Nutkins, J. C., Mortishire-Smith, R. J., Packman, L. C., Brodey, C. L., Rainey, P. B., Johnstone, P. and Williams, D. H. 1991. Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogenPseudomonas tolaasii Paine. J. Amer. Chem. Soc.113: 2621–2627.
Otjen, L. and Blanchette, R. A. 1986. A discussion of microstructural changes in wood during decomposition by white rot basidiomycetes. Can. J. Bot.64: 905–911.
Pelezar, M. J. J. (ed.) 1957. “Manual of microbiological methods,” Society of American Bacteriologists Committee on Bacteriological Technic. McGraw-Hill Book, New York. 315 p.
Rainey, P. B., Brodey, C. L. and Johnstone, K. 1991. Biological properties and spectrum of activity of tolaasin, a lipodepsipeptide toxin produced by the mushroom pathogenPseudomonas tolaasii. Phys. Molec. Pl. Pathol.39: 57–70.
Schaad, N. W. 1988. “Laboratory guide for identification of plant pathogenic bacteria, 2nd ed.,” APS Press, St. Paul. 158 p.
Suyama, K. and Fujii, H. 1993. Bacterial disease occurred on cultivated mushroom in Japan. J. Agri. Sci. Tokyo Nogyo Daigaku38: 35–50. (In Japanese.)
Tolaas, A. G. 1915. A bacterial disease of cultivated mushrooms. Phytopathology5: 51–54.Quercus serrata sapwood byLentinus edodes and its inhibition by an antagonistic hyphomycete,Leptodontidium elatius. Can. J. Bot.25: 36–48.
Tsuneda, A. and Thorn, R. G. 1995. Interactions of wood decay fungi with other microorganisms; with emphasis on the degradation of cell walls. Can. J. Bot.73: In Press.
Tsuneda, A. and Thorn, R. G. 1994. Interactions betweenLentinula edodes and pseudomonads. Can. J. Microbiol.40: 937–943.
Tsuneda, A., Maekawa, N., Ohira, I. and Furukawa, I. 1991. Incipient decay of van Zaayen, A. and Waterreus, H. A. J. I. 1974. Intracellular occurrence of bacteria in mummy-diseased mushrooms. Phytopathology64: 1474–1475.
Wong, W. C. and Preece, T. F. 1979. Identification ofPseudomonas tolaasii: the white line in agar and mushroom tissue block rapid pitting. J. Appl. Bacteriol.47: 401–407.
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Paper No. 301 of the Tottori Mycological Institute.
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Tsuneda, A., Suyama, K., Murakami, S. et al. Occurrence ofPseudomonas tolaasii on fruiting bodies ofLentinula edodes formed onQuercus logs. Mycoscience 36, 283–288 (1995). https://doi.org/10.1007/BF02268603
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DOI: https://doi.org/10.1007/BF02268603