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Packing and performance of microbore columns for adsorption and partition chromatography

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Summary

Microbore columns of 1 mm i.d. have turned out to be very suitable for the achievement of efficient columns.

The packing procedure for stainless steel 1 mm i.d. columns from 5 to 100 cm in length was studied. Stationary phases used were: pure silica gel, octyl, octadecyl and amino bonded silicas. The main parameters (slurry composition, packing system, choice of materials) are discussed.

Short columns packed with 3 or 5μm particles allow high speed separations. A separation in 18 seconds is described.

Very high plate numbers can be obtained with long columns. With 7–8μm particles, a 1 m column can produce 50,000 plates (h=3). Columns can be joined without loss of efficiency. 270 000 theoretical plates were obtained on a 6 m adsorption column with a test mixture. In reversed-phase chromatography, bile acid sodium salts can be separated on a 1 m column. In adsorption chromatography, details are given of the separation of a polystyrene oligomer sample, as well as a light and a heavy petroleum distillate samples on a 2 m column with refractive index detection in the last-case.

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Authors and Affiliations

  1. Laboratoire de Chimie Analytique de l'Ecole Supérieure de Physique et de Chimie Industrielles de la Ville de Paris, 10 rue Vauquelin, 75231, Paris Cedex 05, France

    H. Menet, P. Gareil, M. Caude & R. Rosset

Authors
  1. H. Menet
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  2. P. Gareil
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  3. M. Caude
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  4. R. Rosset
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Menet, H., Gareil, P., Caude, M. et al. Packing and performance of microbore columns for adsorption and partition chromatography. Chromatographia 18, 73–80 (1984). https://doi.org/10.1007/BF02268460

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  • DOI: https://doi.org/10.1007/BF02268460

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