Abstract
[3H]GA20 (1)1, fed toVicia faba seedlings, was converted to [3H]GA20 glucosyl ester (5) and [3H]GA20-13-0-glucoside (6). The GA20 glucosyl ester (5) was identified by HPLC-RC and by GC-MS of GA20-Me formed by transesterification of (5). The [3H]GA20-Me was crystallized to constant specific radioactivity with authentic GA20-Me. On HPLC-RC the GA20-13-0-glucoside (6) was shown to have the same retention time as an authentic sample. Subsequent enzymic hydrolysis gave a product with an HPLC retention time identical to that of authentic GA20 (1).
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Lattke, P., Schneider, G. Formation of GA20 glucosyl conjugates in seedlings ofVicia faba . J Plant Growth Regul 4, 71–79 (1985). https://doi.org/10.1007/BF02266945
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DOI: https://doi.org/10.1007/BF02266945