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Derivatisation of sympathomimetics with primary amino groups on thin-layer plates by spotting the sample spots with fluorescamine

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Summary

A method has been developed for the quantitative determination of sympathomimetics containing a primary amino group on thin-layer plates with fluorescamine. The reaction is carried out by spotting fluorescamine solution in acetone on top of the sample spots. The fluorescamine derivatives are subsequently separated using appropriate solvent systems. Spotting a buffer before reaction and spraying with triethanolamine after development is unnecessary. The consumption of reagent is extremely low. For ten thin-layer plates with ten sample spots per plate only 0.2 cm3 reagent solution are needed. The method has been applied to the quantitative determination of some compounds with primary amino groups in pharmaceutical preparations.

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Authors and Affiliations

  1. Institut für Pharmazeutische Chemie, Universität Graz, A-8010, Graz, Austria

    R. Wintersteiger, G. Gübitz & A. Hartinger

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  1. R. Wintersteiger
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  2. G. Gübitz
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  3. A. Hartinger
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Dedicated to Prof. Dr. G. Zigeuner on his 60th birthday.

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Wintersteiger, R., Gübitz, G. & Hartinger, A. Derivatisation of sympathomimetics with primary amino groups on thin-layer plates by spotting the sample spots with fluorescamine. Chromatographia 13, 291–294 (1980). https://doi.org/10.1007/BF02265644

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  • DOI: https://doi.org/10.1007/BF02265644

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