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Enzyme linked immunosorbent assay for detection of antibodies againstBesnoitia besnoiti in cattle

La Methode Elisa Pour Deceler Les Anticorps ContreBesnoitia besnoiti Chez Les Bovins

Tecnica Immunoenzimatica (Elisa) Para La Deteccion De Anticuerpos ContraBesnoitia besnoiti En Bovinos

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Summary

The use of the ELISA method for the detection of antibodies toB. besnoiti in cattle is described and compared to the IFAT technique. One hundred and twenty-one sera were examined, of which 61 were sera of calves experimentally infected withB. besnoiti, 52 sera from field animals and eight were sera with high titres of antibodies to other parasites. The specificity of both assays correlates but ELISA seemed to be more sensitive. The ELISA technique provides a rapid and reliable method for the screening ofB. besnoiti infection in cattle.

Résumé

L'utilisation de la méthode ELISA pour déceler les anticorps contreB. besnoiti chez les bovins est décrite et comparée à la technique d'agglutination et d'immunofluorescence. Cent vingt et un sérums ont été examinés dont soixante et un provenaient de veaux infectés expérimentalement avecB. besnoiti, cinquante deux d'animaux sur le terrain et huit qui avaient des taux élevés d'anticorps contre d'autres parasites. La spécificité des deux essais est en corrélation mais la méthode ELISA a paru plus sensible et elle est rapide et sûre pour la mise en évidence de l'infection àB. besnoiti chez les bovins.

Resumen

Se describe el uso de método ELISA para detectar anticuerpos deB. besnoiti en bovinos, y se compara este con la técnica IFAT. Se examinaron 121 sueros, de los cuales 61 eran de terneros infectados experimentalmente conB. besnoiti, 52 sueros de animales de campo y ocho sueros con títulos altos, de anticuerpos de otros parásitos. Hubo correlación en la especificidad de ambas pruebas, pero la técnica ELISA pareció más sensitiva. Esta técnica proporciona un método rápido y sensitivo para el sondéo de la infección deB. besnoiti en bovinos.

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Authors and Affiliations

  1. Kimron Veterinary Institute, Beit Dagan, Israel

    Varda Shkap, Hanna Ungar-Waron & E. Pipano

  2. Hadassah Medical School, Hebrew University, Jerusalem, Israel

    C. Greenblatt

Authors
  1. Varda Shkap
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  2. Hanna Ungar-Waron
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  3. E. Pipano
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  4. C. Greenblatt
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Shkap, V., Ungar-Waron, H., Pipano, E. et al. Enzyme linked immunosorbent assay for detection of antibodies againstBesnoitia besnoiti in cattle. Trop Anim Health Prod 16, 233–238 (1984). https://doi.org/10.1007/BF02265330

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  • DOI: https://doi.org/10.1007/BF02265330

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