Summary
The glycoforms contained within each glycosylation site of orosomucoid can be separated by reverse-phase HPLC without removal of the associated peptide or chemical derivatization. The chromatographic properties of the glycopeptides are dominated by the peptide portion but modified by the hydrophilicity of the glycan in a regular manner depending on its size. Assignments for known structures are readily made, permitting quantitative assessment of changes in glycoform concentration at individual glycosylation sites.
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Treuheit, M., Halsall, H.B. HPLC of individual site glycoforms in serum orosomucoid. Chromatographia 31, 478–480 (1991). https://doi.org/10.1007/BF02262392
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DOI: https://doi.org/10.1007/BF02262392