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The measurement of temperature inside capillaries for electrophoresis using thermochromic solutions

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Summary

Capillary temperature is one of the most important parameters in capillary electrophoresis (CE). Its control is essential. The efficiency of different methods of thermostatic control are difficult to compare because the measurement of temperature inside the capillary has previously only been possible using indirect methods. Direct measurement of the temperature is now possible using the thermochromic effect. Cobalt(II)chloride solutions change their absorption spectrum distinctively with changes in temperature. The results obtained by this method show an increase in temperature of several degrees Kelvin despite thermostatic control. This is in good agreement with theoretical calculations [1, 13].

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  1. Institute of Pharmacy and Food Chemistry, University of Würzburg, Am Hubland, 8700, Würzburg, Germany

    H. Wätzig

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  1. H. Wätzig
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Wätzig, H. The measurement of temperature inside capillaries for electrophoresis using thermochromic solutions. Chromatographia 33, 445–448 (1992). https://doi.org/10.1007/BF02262325

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  • DOI: https://doi.org/10.1007/BF02262325

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