Summary
Examples of high-performance liquid chromatography (HPLC) are given for the determination of adsorption equilibrium and distribution coefficients, calculation of excess adsorption isotherms from solutions ranging from 10−7 to 10−1 mole fraction, changes in activity coefficient of the component predominantly adsorbed from solution and substitution enthalpy. These examples stem from studies of HPLC and the static adsorption of benzene, anisole and benzaldehyde from solutions in n-heptane on macroporous silica and active carbon at various temperatures. A criterion is suggested for evaluating equilibrium in the HPLC process.
The potential of size-exclusion chromatography (SEC) for evaluating the volume and predominant diameter of adsorbed pores as well as pore diffraction are discussed. Prospects for HPLC application to molecularstructural analysis and other purposes are discussed briefly.
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Eltekova, N.A., Eltekov, Y.A. Physico-chemical applications of HPLC. Chromatographia 27, 633–638 (1989). https://doi.org/10.1007/BF02258994
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DOI: https://doi.org/10.1007/BF02258994