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Automated analysis of terbutaline (BricanylR) in human plasma with liquid chromatography and electrochemical detection using column-switching (multidimensional chromatography)

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Summary

An automated method based on liquid chromatography has been developed for the determination of terbutaline in human plasma in the range of 5–50 pmole·ml−1. The necessary sensitivity and selectivity was obtained by using electrochemical detection and a microprocessor-controlled column switching system. A combination of three columns was used: a C8 type for pre-separation, a C18 type for trapping and, for final separation, a strongly acidic ion exchanger. The accuracy of the method was examined by comparison with a method based on gas chromatography — mass spectrometry. The overall precision was ±3.5% and ±2.2% respectively at 5 and 50 pmole·ml−1. The total absolute recovery for terbutaline and internal standard at the above concentration levels were in the range 85–106%.

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References

  1. L.-Å. Svensson, A. Björklund, O. Lindvall, Acta Chem. Scand.B29, 341 (1975).

    Google Scholar 

  2. J. G. Leferink, J. Wagemaker-Engels, R. A. A. Maes, H. Lamont, R. Pauwels, M. Van der Straeten, J. Chromatogr.143, 299 (1977).

    Google Scholar 

  3. R. A. Clare, D. S. Davies, T. A. Baillie, Biomed. Mass Spectrom.6, 31 (1979).

    Google Scholar 

  4. S.-E. Jacobsson, S. Jönsson, C. Lindberg, L.-Å. Svensson, Bio-med. Mass Spectrom.7, 265 (1980).

    Google Scholar 

  5. S. Bergquist, L.-E. Edholm. Submitted for publication in J. Liq. Chromatogr.

  6. J. F. K. Huber, R. Hirz. Paper presented at the VI International Symposium on Column Liquid Chromatography, Philadelphia, June 1982).

  7. L.-E. Edholm. Unpublished data.

  8. B. A. Bidlingmeyer, J. K. Del Rios, J. Korpl, Anal. Chem.54, 442 (1982).

    Google Scholar 

  9. S. Eksborg, Clin. Chem.27 (7), 1311 (1981).

    Google Scholar 

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Authors and Affiliations

  1. Pharmacokinetic Laboratory, AB Draco, P.O.B. 1707, S-22101, Lund, Sweden

    L. -E. Edholm & B. -M. Kennedy

  2. Technical Analytical Chemistry, Chemical Center, P.O.B. 740, S-22007, Lund, Sweden

    S. Bergquist

Authors
  1. L. -E. Edholm
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  2. B. -M. Kennedy
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  3. S. Bergquist
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Edholm, L.E., Kennedy, B.M. & Bergquist, S. Automated analysis of terbutaline (BricanylR) in human plasma with liquid chromatography and electrochemical detection using column-switching (multidimensional chromatography). Chromatographia 16, 341–344 (1982). https://doi.org/10.1007/BF02258934

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  • DOI: https://doi.org/10.1007/BF02258934

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