Zooplankton grazing methods using radioactive tracers: Technical problems

Abstract

Some of the technical problems specific to the application of radiotracer techniques for measuring grazing of zooplankton are highlighted and, in some cases, the corrective measures suggested.

The major drawbacks of measuring zooplankton grazing in the laboratory, for studying the role of zooplankton in nature, namely, changes in water temperature and light and heavy mortality of some cladocerans, are overcome by the use ofin situ grazing chambers. However, at present there are no suitable techniques available to measure short-term assimilation rates which usually suffer from the respiratory loss during the experiment of the assimilated radiotracer.

In the ecosystem research in which zooplankton grazing is an integral part of the studies, the radiotracer food manufactured fromin situ lake seston is preferred to labelled monoculture of algae, bacteria or yeast. The specific activities of the different tracer components and size fractions of the seston comprising the tracer may considerably differ in short-term labelling. On the other hand, long labelling periods,i.e. exceeding a day for example, may lead to shifts in size spectra of and composition of the different phytoplankton species. Changes during the incubation in the size spectra of the tracerfood are probably of greater importance than those in species composition, especially because a size-selective feeding may often exist.

Simultaneous use of different radiotracers for labelling phytoplankton and bacteria or for mixtures of algae that differ in sizes offers some promising applications. More work in this field is needed, particularly because of the overlapping energy spectra of the radiotracers, and differential loss rates of these tracers from the preserved animals before the radioactivity counting. Such losses of the tracer from the experimental animals may lead to significant errors, especially in the short-term grazing measurements. This problem can be, nevertheless, prevented to a large extent by a rapid freeze-drying of the animals immediately on concluding the experiment.

Self absorption may be a problem in radioactivity counting, more so if the animals are large. Use of sample oxidizers offers a good solution if the tracer can be trapped in its oxidized, gaseous form (e.g. ¹⁴CO₂) before further processing for counting. Modern solubilizing agents, like Soluene 350, with their relatively high water-holding capacity are quite effective in dissolving the different tissues, except the exoskeletal material.