Abstract
Retinol-binding protein (RBP) is a marker of tubular reabsorption in the kidneys. The aim of our study was to investigate urinary RBP excretion in healthy children to obtain reference values related to age and pubertal stage. Overnight samples from 143 subjects (73 girls, 70 boys) aged 10–18 years were investigated. RBP was quantified by a solid-phase sandwich enzyme immunoassay. Both the RBP excretion rate and the RBP/ creatinine ratio (RBP/Cr) showed a skewed distribution. The medians and the 5th–95th percentiles were 38 ng/min (15–127) and 9 μg/mmol (4–23), respectively. The RBP excretion rate and RBP/Cr ratio were similar in both sexes, and linear multiple regression analysis showed no association with age or pubertal stage, although a weak relationship (r = 0.27) was found between RBP excretion rate and age in boys and RBP/Cr ratio and age (r = -0.28) in girls by simple correlation analysis. The correlation between RBP excretion rate and RBP/Cr ratio was 0.76; the RBP excretion rate and RBP/Cr ratio measured on 2 consecutive days, showed a correlation coefficient of 0.84 and 0.88, respectively. We conclude that overnight RBP excretion in children over 10 years shows a low day-to-day variation and, in practical terms, is independent of age, gender and pubertal stage.
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References
Rowe DJF, Anthony F, Polak A, Shaw K, Ward CD (1976) Retinol-binding protein as a small molecular weight marker of renal function in diabetes mellitus. Ann Clin Biochem 24: 477–482
Mutti A, Alinovi R, Ghiggeri GM, Bergamaschi E, Candiano G, Rasi A, Gusmano R, Franchini I, Borghetti A (1990) Urinary excretion of brushborder antigen and plasma proteins in early stages of diabetic nephropathy. Clin Chim Acta 188: 93–100
Bernard AM, Moreau D, Lauwerys R (1982) Comparison of retinol-binding protein and B2-microglobulin determination in urine for the early detection of tubular proteinuria. Clin Chim Acta 126: 1–7
Jensen T, Deckert M, Dawnay A, Feldt-Rasmussen B (1989) Micro-ELISA for the quantitation of human urinary and serum retinol-binding protein. Diabetes Res 10: 93–95
Topping MD, Forster HW, Dolman C, Luczymska CM, Bernard AM (1986) Measurement of urinary retinol-binding protein by enzyme-linked immunosorbent assay and its application to detection of tubular proteinuria. Clin Chem 32: 1863–1866
Thakkar H, Cornelius J, Dronfield DM, Medcalf EA, Newman DJ, Price CP (1991) Development of a rapid latex enhanced turbidimetric assay for retinol binding protein in urine. Ann Clin Biochem 28: 407–411
Smith GC, Winterborn MH, Taylor CM, Lawson N, Guy M (1994) Assessment of retinol-binding protein excretion in normal children. Pediatr Nephrol 8: 148–150
Gibb DM, Tomlinson PA, Dalton NR, Turner C, Shah V (1989) Renal tubular proteinuria and microabluminuria in diabetic patients. Arch Dis Child 64: 129–134
Tomlinson PA, Dalton RN, Turner C, Chantler C (1990) Measurement of β2-rnicroglobulin, retinol-binding protein, α1-micro-globulin and urine protein 1 in healthy children using enzymelinked immunosorbent assay. Clin Chim Acta 192: 99–106
Piqueras I, Shah V, Hulton SA, Barratt TM, Dillon MJ (1993) Tubular proteinuria in steroid sensitive multi-relapsing nephrotic syndrome. Clin Nephrol 40: 26–30
Ginevri F, Piccotti E, Alinovi R, DeToni T, Biagini C, Chiggeri GM, Gusmano R (1993) Reversible tubular proteinuria precedes microalbuminuria and correlates with the metabolic status in diabetic children. Pediatr Nephrol 7: 23–26
Smith ST (1985) Nonprotein nitrogen. In: Bishop ML, Duben-Von Lamfen JH, Fody EP (eds) Clinical chemistry, principles, procedures, correlations. Lippincott, Philadelphia, pp 414–416
Elston RC, Johnson WC (1987) Essentials of biostatistics. Navis, Philadelphia, pp 249–250
Lehman EL (1975) Statistical methods based on ranks. Holden-Day, San Fransisco, pp 181–185
Dahlquist G, Rudberg S (1987) The prevalence of microalbuminuria in diabetic children and adolescents and its relation to puberty. Acta Paediatr Scand 76: 795–800
Mortensen HB, Martinelli K, Nørgaard K, Main K, Kastrup KW, Ibsen KK, Villumsen J, Parving H-H (1990) A nation-wide crosssectional study of urinary albumin excretion rate, arterial blood pressure and blood glucose control in Danish children with type 1 diabetes mellitus. Diabetic Med 7: 887–897
Bangstad H-J, Dahl-Jorgensen K, Kjærsgaard P, Hanssen K (1993) Urinary albumin excretion rate and puberty in non-diabetic children and adolescents. Acta Pacdiatr 82: 857–862
Williamson JR, Chang K, Tilton RG, Prater C, Jeffrey JR, Weigel C, Sherman WR, Eades DM, Kilo C (1987) Increased vascular permeability in spontaneously diabetic BB/W rats and in rats with mild versus severe streptozocin-induced diabetes. Diabetes 36: 813–821
Bernard A, Lauwerys R, Noel A, Vandeleene B, Lambert A (1989) Urine protein 1: a sex-dependent marker of tubular or glomerular dysfunction. Clin Chem 35: 2141–2142
Feldt-Rasmussen B, Mathiesen ER (1984) Variability of urinary excretion in incipient diabetic nephropathy. Diabet Nephropathy 3: 101–103
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Bangstad, HJ., Kierulf, P., Kjærsgaard, P. et al. Urinary excretion of retinol-binding protein in healthy children and adolescents. Pediatr Nephrol 9, 299–302 (1995). https://doi.org/10.1007/BF02254190
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DOI: https://doi.org/10.1007/BF02254190