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Peptide inhibitor of interleukin-8 (IL-8) reduces staphylococcal enterotoxin-A (SEA) induced neutrophil trafficking to the lung

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Abstract

Staphylococcal enterotoxin A (SEA) is a superantigen, produced by some strains ofStaphylococcus aureus (S. aureus), which can cause a variety of clinical manifestations ranging from food poisoning to shock. SEA can also stimulate human alveolar macrophages to produce interleukin-8 (IL-8), a member of the α-chemokine subfamily that activates and is chemotactic for neutrophils. In these studies we showed that in rabbits, intravenous SEA significantly decreased the circulating white blood cell population from a baseline value of 6409±2027×103 cells/ml to 1943±862×103 cells/ml in 7 h. There was a concommitent increase in IL-8 in the circulating plasma (baseline: 60±34 pg/ml, 7 h post SEA: 109±64 pg/ml). The increase in circulating IL-8 was accompanied by a much greater increase in the IL-8 concentration of the epithelial lining fluid (ELF) where the IL-8 increased from 0.05±0.08 ng/ml (control) to 13.8±9.3 ng/ml (SEA treated). The increase in IL-8 concentration in the alveolar spaces was paralleled by an increase in both the percentage of neutrophils (1.4±0.9% (control) to 26.0±10.8% (SEA treated)) and total number of neutrophils (0.04±0.02×106/ml (control) to 4.8±3.3 106/ml (SEA treated)) in the airspaces, and the numbers of neutrophils in the ELF correlated with the IL-8 concentration r=0.62, p=0.006). When antileukinate, a hexapeptide which inhibits the binding of IL-8 to neutrophils, was administered to animals receiving SEA, the IL-8 concentration in the ELF (14.8±10.7 ng/ml) was not significantly different from the concentration of IL-8 in those animals receiving SEA alone). However, both the percentage of neutrophils (9.5±3.2%), and the total number of neutrophils (1.3±1.0×106/ml) in the ELF following SEA and antileukinate administration was significantly lower than in animals which only received SEA (p<0.05). The findings suggest that SEA released into the circulation during a Staphylococcal infection can cause an inflammatory reaction in the lung. Since this reaction is at least partially mediated by IL-8, antileukinate may have pharmacologic potential in reducing the inflammatory reaction.

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Authors and Affiliations

  1. Departments of Biochemistry, University of Texas Health Center, 75710-2003, Tyler, TX, USA

    E. J. Miller & A. B. Cohen

  2. Department of Medicine, University of Texas Health Center, 75710-2003, Tyler, TX, USA

    A. B. Cohen

  3. Department of Physiology, University of Texas Health Center, 75710-2003, Tyler, TX, USA

    B. T. Peterson

Authors
  1. E. J. Miller
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  2. A. B. Cohen
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  3. B. T. Peterson
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accepted by I. Ahnfelt-Rønne

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Miller, E.J., Cohen, A.B. & Peterson, B.T. Peptide inhibitor of interleukin-8 (IL-8) reduces staphylococcal enterotoxin-A (SEA) induced neutrophil trafficking to the lung. Inflamm Res 45, 393–397 (1996). https://doi.org/10.1007/BF02252934

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