Abstract
l-Valine competes with tryptophan for transport into the brain and has previously been shown to decrease brain 5-HT synthesis. In the present study, the effect ofl-valine on electrically evoked hippocampal 5-HT release was determined in the anaesthetized rat using microdialysis. In control animals two electrical stimulations of the dorsal raphe nucleus 120 min apart (S1 and S2, respectively) released similar amounts of 5-HT. In contrast, in animals which receivedl-valine (200 mg/kg) between stimulations, S2 released a significantly smaller amount of 5-HT than did S1, although basal 5-HT release was unchanged. The data demonstrate thatl-valine decreases the electrically-evoked release of 5-HT in hippocampus in vivo.
References
Delgado PL, Charney DS, Price LH, Aghajanian GK, Landis H, Heninger GR (1990) Serotonin function and the mechanism of antidepressant action. Reversal of antidepressant-induced remission by rapid depletion of plasma tryptophan. Arch Gen Psychiatry 47:411–418
Fernstrom JD, Faller DV (1978) Neutral amino acids in the brain: changes in response to food injestion. J Neurochem 30:1531–1538
Fernstrom JD, Wurtman RJ (1971) Brain serotonin content: physiological dependence on plasma tryptophan levels. Science 173:149–152
Gessa GL, Biggio G, Fadda F, Corsini GV, Tagliamonte A (1974) Effect of oral administration of tryptophan-free amino acid mixtures on serum tryptophan, brain tryptophan and serotonin metabolism. J Neurochem 22:869–870
Joseph MH, Kennett GA (1983) Corticosteroid response to stress depends upon increased tryptophan availability. Psychopharmacology 79:79–81
Kennett GA, Joseph MH (1981) The functional importance of increased brain tryptophan in the serotonergic response to restraint stress. Neuropharmacology 20:39–43
Kuhn DM, Wolf WA, Youdim MBH (1986) Serotonin neurochemistry: a new look at some old axioms. Neurochem Int 8:141–154
Lookingland KJ, Shannon NJ, Chapin DS, Moore KE (1986) Exogenous tryptophan increases synthesis, storage, and intraneuronal metabolism of 5-hydroxytryptamine in the rat hypothalamus. J Neurochem 47:205–212
Moir ATB, Eccleston D (1968) The effect of precursor loading in the cerebral metabolism of 5-hydroxyindoles. J Neurochem 15:1093–1108
Paxinos S, Watson C (1982) The rat brain in stereotaxic co-ordinates. Academic Press, Sydney
Schaechter JD, Wurtman RJ (1990) Serotonin release varies with brain tryptophan levels. Brain Res 532:203–210
Sharp T, Bramwell SR, Grahame-Smith DG (1990) Release of endogenous 5-hydroxytryptamine in rat ventral hippocampus evoked by electrical stimulation of the dorsal raphe nucleus as detected by microdialysis: sensitivity to tetrodotoxin, calcium and calcium antagonists. Neuroscience 39:629–637
Sharp T, Bramwell SR, Grahame-Smith DG (1992) Effect of acute administration ofl-tryptophan on the release of 5-HT in rat hippocampus in relation to serotoninergic neuronal activity: an in vivo microdialysis study. Life Sci 50:1215–1223
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Gartside, S.E., Cowen, P.J. & Sharp, T. Evidence that the large neutral amino acidl-valine decreases electrically-evoked release of 5-HT in rat hippocampus in vivo. Psychopharmacology 109, 251–253 (1992). https://doi.org/10.1007/BF02245511
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DOI: https://doi.org/10.1007/BF02245511