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In vitro demonstration of delayed hypersensitivity to gastric antigens in pernicious anemia

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Abstract

Migration inhibition of peripheral leukocytes in the presence of various human gastric antigens was looked for in 12 patients with pernicious anemia and 8 healthy controls as an indication of the presence of abnormal delayed hypersensitivity in this disease. Gastric juice antigens caused migration inhibition in 9 of 12 patients with pernicious anemia and in 1 healthy relative of a patient with pernicious anemia at doses which did not cause migration inhibition in control subjects. Fractionation of the gastric juice showed that the antigens causing migration inhibition were in both the vitamin B12 binding and the nonvitamin B12 binding fractions. Where the vitamin B12 binding fraction had been saturated with vitamin B12, migration inhibition was still seen. There was no relation between the presence of parietal cell or intrinsic factor antibodies and migration inhibition in response to gastric juice antigens. This work confirms that abnormal delayed hypersensitivity occurs in pernicious anemia and, in addition, suggests that several antigens may be involved in these reactions.

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Authors and Affiliations

  1. From the Division of Gastroenterology, Department of Medicine, Cornell University Medical College, and the New York Blood Center, NY

    N. D. C. Finlayson BSc (Hons), MB, ChB, MD (MRCP), M. H. Fauconnet (Technician) & Kai Krohn MD

Authors
  1. N. D. C. Finlayson BSc (Hons), MB, ChB, MD
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  2. M. H. Fauconnet
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  3. Kai Krohn MD
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Additional information

Supported in part by Training Grant T01-AM-5430, Institute of Arthritis and Metabolic Diseases, and Grant HE 09011-07, National Heart and Lung Institute, National Institutes of Health.

Kai Krohn, MD, was the recipient of Public Health Service International Fellowship No. 7F05 TW 1547-01.

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Finlayson, N.D.C., Fauconnet, M.H. & Krohn, K. In vitro demonstration of delayed hypersensitivity to gastric antigens in pernicious anemia. Digest Dis Sci 17, 631–638 (1972). https://doi.org/10.1007/BF02231750

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  • DOI: https://doi.org/10.1007/BF02231750

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