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Cathepsin G and elastase in synovial fluid and peripheral blood in reactive and rheumatoid arthritis

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The purpose of the study was to evaluate the involvement of serine proteinases cathepsin G and elastase on pathomechanisms in synovial fluid (SF) of patients with reactive (ReA) and rheumatoid, (RA) arthritis. Cathepsin G, elastase, and their endogenous inhibitors α1-antichymotrypsin (α1-ACT) and α1-proteinase inhibitor (α1-PI) were identified immunohistochemically from SF and peripheral blood (PB) of patients with ReA and RA. Cathepsin G and elastase activities in SF and PB were measured spectrophotometrically. Dot-immunostaining was used to identify cathepsin G, elastase, but also α1-ACT and α1-PI from SF and PB. Cathepsin G and elastase-like activities (IU/I) were slightly elevated in ReA SF compared to the corresponding peripheral blood values (11.4±9.2 vs 4.8±1.7, NS, and 5.1±2.8 vs 2.3±2.2, NS), which was similar to what was seen in RA (16.4±6.2 vs 0.53±0.4, p<0.05, and 6.51±1.8 vs 1.22±0.58, p<0.05). Although some samples did not contain cathepsin G and/or elastase-like activities, all samples contained immunoreactive enzyme, but also α1-ACT and α1-PI. In ReA SF, in contrast to monocytes, all polymorphonuclear (PMN) cells contained cathepsin G and elastase. Cathepsin G and elastase activities correlated with each other (r=0.78, p<0.05) suggesting PMN / primary granules as their likely source. There was a closer association between the cathepsin G or elastase and SF leukocyte count in ReA than in RA. In ReA and RA SF elevated cathepsin G and elastase activities are detected compared to activity levels in PB suggesting local production mainly from PMNs. The co-existence of highly cellular SF and cathepsin G and elastase activity in the documented presence of endogenous inhibitors in ReA SF together with the, known, usually self-remitting clinical course of ReA, suggest a brisk and even exaggerated local PMN serine proteinase release; sparing of joints does not seem to be due to lack or inhibition of PMN responses but rather to a successful down-regulation or cessation of the responses initially elicited.

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Authors and Affiliations

  1. Institute of Biomedicine, Department of Anatomy, University of Helsinki, Siltavuorenpenger 20 A, FIN-00014, Finland

    D. Nordström M.D., O. Lindy, Y. T. Konttinen & T. Sorsa

  2. Orthopaedic Hospital of the Invalid Foundation, Tenholantie 10, FIN-00280, Helsinki, Finland

    S. Santavirta

  3. Dept. of Medicine Division of Rheumatology, Helsinki University Central Hospital, Kasarmink, 11-13, FIN-00140, Helsinki, Finland

    D. Nordström M.D., Y. T. Konttinen, A. Lauhio, C. Friman & T. Pettersson

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Nordström, D., Lindy, O., Konttinen, Y.T. et al. Cathepsin G and elastase in synovial fluid and peripheral blood in reactive and rheumatoid arthritis. Clin Rheumatol 15, 35–41 (1996). https://doi.org/10.1007/BF02231682

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