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Isolation and transmission ofCowdria ruminantium (causal agent of heartwater disease) in Blue Nile Province, Sudan

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Abstract

Heartwater was diagnosed in sheep at the Umm Banein Livestock Research Station, Blue Nile Province, Sudan.Cowdria ruminantium was isolated from sheep by subinoculation of blood into a goat. AdultAmblyomma lepidum ticks fed as nymphs on this goat transmitted heartwater to a Friesian (Bos taurus) calf. Brain stabilates from this calf, either snapfrozen into liquid nitrogen or frozen slowly in a deepfreezer at −70°C, with DMSD as cryoprotectant, were infective to cattle when inoculated subcutaneously.

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Jongejan, F., Morzaria, S.P., Shariff, O.A. et al. Isolation and transmission ofCowdria ruminantium (causal agent of heartwater disease) in Blue Nile Province, Sudan. Vet Res Commun 8, 141–145 (1984). https://doi.org/10.1007/BF02214705

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