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Cholecystokinin-8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation

  • Pancreatic and Biliary Disorders
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Abstract

To study the early pathogenesis of acute edematous pancreatitis in dogs, we examined the relationship of pancreatic hyperstimulation with cholecystokinin-8 (10 μg/kg/hr intravenously for 6 hr) to alterations in circulating pancreatic enzymes and pancreatic morphology with special reference to trypsinogen activation. Cholecystokinin-8 infusion was associated with increases in plasma amylase, lipase, trypsin-like immunoreactivity, and plasma and urine trypsinogen activation peptide. Pancreatic parenchymal swelling and interlobular and subcapsular fluid accumulations were detected ultrasonographically within 2 hr of cholecystokinin-8. Circulating trypsin-like immunoreactivity and trypsinogen activation peptide in urine reached a peak at 2 and 4 hr, respectively, then declined despite progressive increases in circulating amylase and lipase and intrapancreatic fluid. No significant changes were observed in dogs receiving a saline infusion. This study illustrates that cholecystokinin-8 induces edematous pancreatitis in dogs that is associated with a short-lived burst of trypsinogen activation.

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Authors and Affiliations

  1. Departments of Small Animal Medicine and Surgery, and Pathology, Royal Veterinary College, U.K., U.K.

    K. W. Simpson BVMTS, PhD, N. Beechey-Newman FRCS, C. R. Lamb MA, VetMB, J. B. A. Smyth MVB, MSc, PhD, G. Hughes HND, K. Coombe BSc, N. Sumar PhD & J. Hermon-Taylor MChir, FRCS

  2. Departments of Surgery, Guy's and St. George's Hospital Medical Schools, University of London, U.K.

    K. W. Simpson BVMTS, PhD, N. Beechey-Newman FRCS, C. R. Lamb MA, VetMB, J. B. A. Smyth MVB, MSc, PhD, G. Hughes HND, K. Coombe BSc, N. Sumar PhD & J. Hermon-Taylor MChir, FRCS

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  1. K. W. Simpson BVMTS, PhD
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  2. N. Beechey-Newman FRCS
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  3. C. R. Lamb MA, VetMB
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  4. J. B. A. Smyth MVB, MSc, PhD
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  5. G. Hughes HND
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  6. K. Coombe BSc
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  7. N. Sumar PhD
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  8. J. Hermon-Taylor MChir, FRCS
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Financial support for this study was provided in part by the Wellcome Trust.

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Simpson, K.W., Beechey-Newman, N., Lamb, C.R. et al. Cholecystokinin-8 induces edematous pancreatitis in dogs associated with short burst of trypsinogen activation. Digest Dis Sci 40, 2152–2161 (1995). https://doi.org/10.1007/BF02208999

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  • DOI: https://doi.org/10.1007/BF02208999

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