Abstract
Vesicular-arbuscular mycorrhizal (VAM) associations often vary according to the abundance of available soil phosphorus (P). Therefore, understanding the response of crop plants to colonization by VAM fungi necessitates the study of the response of colonized and noncolonized plants, from a range of cultivars, to differing levels of P. Cowpea is grown throughout the world, often on impoverished soils in which it can benefit from formation of mycorrhizae. The present study was conducted to determine the response of four cultivars of cowpea (Vigna unguiculata (L.) Walp.), varying in nitrogen fixation capacity, to inoculation withGlomus fasciculatum at four levels of added P in the rooting medium. In a greenhouse experiment, four cowpea cultivars, Mississippi Silver, Brown Crowder, Six Week Browneye and MI 35, were grown with and without the mycorrhizal fungus at four levels of added P, 0, 10, 20 and 30 ppm. Root colonization (%) was negatively correlated with P content of the growth medium and shoot P concentration. Intraspecific variability was shown for shoot dry weight and leaf area in response to inoculation withG. fasciculatum at different P levels. The range of P required in the growth medium which allowed benefit fromG. fasciculatum was identified for individual cultivars using shoot dry weight and leaf area, and collectively across cultivars for other parameters.
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References
Abbot L K and Robson A D 1984 The effect of VA mycorrhizae on plant growth. pp 113–130.In VA Mycorrhiza. Eds. C L Powell and D J Bagyaraj. CRC Press, Boca Raton, FL.
Asimi S, Gianinazzi-Pearson V and Gianinazzi S 1980 Influence of increasing phosphorus levels on interactions between vesicular-arbuscular mycorrhizae andRhizobium in soybean. Can. J. Bot. 58, 2200–2205.
Bolgiano N C, Safir G R and Warncke D D 1983 Mycorrhizal infection and growth of onion in the field in relation to phosphorus and water availability. J. Am. Soc. Hort. Sci. 108, 819–825.
Buwalda J G, Ross G J S, Stribley D B and Tinker P B 1982 The development of endomycorrhizal root systems. IV. The mathematical analysis of effects of phosphorus on the spread of vesicular-arbuscular mycorrhizal infection in root systems. New Phytol. 92, 391–399.
Daft M J and Nicolson T H 1969 Effect ofEndogone mycorrhiza on plant growth. II. Influence of soluble phosphate on endophyte and host in maize. New Phytol. 68, 945–952.
Daniels B A and Skipper H D 1982 Methods for the recovery and quantitative estimation of propagules from soil. pp 29–35.In Methods and Principles of Mycorrhizal Research. Ed. N C Schenck. Amer. Phytopath. Soc., St. Paul, MN.
Islam R and Ayanaba A 1981 Growth and yield responses of cowpea and maize to inoculation withGlomus mosseae in sterilized soil under field conditions. Plant and Soil 63, 505–509.
Islam R and Ayanaba A 1981 Effect of seed inoculation and preinfecting cowpea (Vigna unguiculata) withGlomus mosseae on growth and seed yield of the plants under field conditions. Plant and Soil 61, 341–350.
Islam R, Ayanaba A and Sanders F E 1980 Response of cowpea (Vigna unguiculata (L.) Walp.) to inoculation with vesiculararbuscular mycorrhizal fungi and to rock phosphate fertilization in some sterilized Nigerian soils. Plant and Soil 54, 107–117.
Manjunath A and Bagyaraj D J 1984 Response of pigeon pea and cowpea to phosphate and dual inoculation with vesiculararbuscular mycorrhiza andRhizobium. Trop. Agr. (Trinidad) 61, 48–52.
Mengel K and Kirby E A 1982 Principles of Plant Nutrition. 3rd ed. International Potash Inst., Switzerland.
Menge J A, Labanauskas C K and Johnson E L V 1978 Partial substitution of mycorrhizal fungi for phosphorus fertilization in the greenhouse culture of citrus. Soil Sci. Soc. Am. J. 42, 926–930.
Menge J A, Sterile D, Bagyaraj D J, Johnson E L V and Leonard R T 1978 Phosphorus concentrations in plants responsible for inhibition of mycorrhizal infection. New Phytol. 80, 575–578.
Nelsen C E, Bolgiano N C, Furutani S C, Safir G R and Zandstra B H 1981 The effect of soil phosphorus levels on mycorrhizal infection of field grown onion plants and on mycorrhizal reproduction. J. Am. Soc. Hort. Sci. 106, 786–788.
Ollivier B, Bertheau Y, Diem H G and Gianinazzi-Pearson V 1983 Influence de la variete deVigna unguiculata dans l'expression de trios associations emdomycorrhiziennes a vesicules et arbuscules. Can. J. Bot. 61, 354–358.
Phillips J M and Hayman D S 1970 Improved procedures for clearing roots and staining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. British Mycol. Soc. 55, 158–161.
Rajapakse S and Miller J C Jr 1987 Intraspecific variability for VA mycorrhizal symbiosis in cowpea (Vigna unguiculata (L.) Walp.). pp 523–536.In Genetic Aspects of Plant Mineral Nutrition. Eds. W H Gabelman and B C Loughman. Martinus Nijhoff, Dordrecht, Netherlands.
SAS Institute Inc. 1985 SAS user's guide: Statistics. 1985 edition. SAS Institute, Inc., Cary, NC.
Sokal R R and Rohlf F J 1981 Biometry: The Principles and Practice of Statistics in Biological Research. 2nd ed. W H Freeman and Co., San Francisco, CA.
Technicon 1975 Industrial Method No. 369-75 A/A. Technicon Industrial Systems, Tarrytown, NY.
Technicon 1977 Industrial Method No. 337-74 W/B. Technicon Industrial Systems, Tarrytown, NY.
Zary K W, Miller J C Jr, Weaver R W and Barnes L W 1978 Intraspecific variability for nitrogen fixation in southern pea (Vigna unguiculata (L.) Walp.). J. Am. Soc. Hort. Sci. 103, 806–808.
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Rajapakse, S., Zuberer, D.A. & Miller, J.C. Influence of phosphorus level on VA Mycorrhizal colonization and growth of cowpea cultivars. Plant Soil 114, 45–52 (1989). https://doi.org/10.1007/BF02203080
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DOI: https://doi.org/10.1007/BF02203080