Abstract
A gene at 42 min on theEscherichia coli chromosome, identified as the locus of pseudoreversion of knockout mutations in the biotin sulfoxide reductase gene,bisC, has 64% base sequence identity withbisC. This makes it a member of a multigene family of molybdopterin enzymes that includes genes for anaerobic reduction of trimethylamine oxide (torA) and dimethylsulfoxide (dmsA). Disruption of this gene eliminates the background activity of biotin sulfoxide reduction observed inbisC mutants. Sequence comparison of the new gene (bisZ) withbisC indicates that certaints mutants ofbisC arise by gene conversion between the two loci.
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Adelberg EA, Mandel M, Chen GCC (1965) Optimal conditions for mutagenesis by N-methyl-N'-nitro-N-nitrosoguanidine inEscherichia coli K-12. Biochem Biophys Res Commun 18:788–795
Berg CM, Wang M, Vartak NB, Liu L (1988) Acquisition of new metabolic capabilities: multicopy suppression by cloned transaminase genes inEscherichia coli K-12. Gene 65:195–202
Berg BL, Li J, Heider J, Stewart V (1991) Nitrate-inducible formate dehydrogenase inEscherichia coli K-12. J Biol Chem 266:22380–22385
Berg JM, Holm RH (1985) A model for the active site of oxo-transfer molybdoenzymes; reactivity, kinetics and catalysis. J Am Chem Soc 107:925–932
Berks BC, Richardson DJ, Reilly A, Willis AC, Ferguson SJ (1995) ThenapEDABC gene cluster encoding the periplasmic nitrate reductase system ofThiosphaera pantotropha. Biochem J 309:983–992
Bilous PT, Cole ST, Anderson WF, Weiner JH (1988) Nucleotide sequence of thedmsABC operon encoding the anaerobic dimethyl sulfoxide reductase ofEscherichia coli HB101. Mol Microbiol 2:785–795
Blasco F, Iobbi C, Giordano G, Chippaux M, Bonnefoy V (1989) Nitrate reductase ofEscherichia coli: completion of the nucleotide sequence of thenar operon and reassessment of the role of the α and β subunits in iron binding and electron transfer. Mol Gen Genet 218:249–256
Blasco F, Iobbi C, Ratouchniak J, Bonnefoy V, Chippaux M (1990) Nitrate reductases ofEscherichia coli; sequence of the secondary nitrate reductase and comparison with that encoded by thenarGHJI operon. Mol Gen Genet 222:104–111
Bouffard G, Ostell J, Rudd KE (1992) Gene Scope: a relational database ofEscherichia coli genomic map data for Macintosh computers. Comput Applic Biosci 8:563–567
del Campillo-Campbell A, Campbell A (1982) Molybdenum cofactor requirement for biotin sulfoxide reduction inEscherichia coli. J Bacteriol 149:379–385
Groisman EA, Castillo BA, Casadaban MJ (1984)In vivo DNA cloning and adjacent gene fusing with a mini-Mu-lac bacteriophage containing a plasmid replicon. Proc Natl Acad Sci USA 81:1480–1483
Henikoff S (1987) Unidirectional digestion with exonuclease III. Methods Enzymol 155:156–165
Kohara Y, Akiyama K, Isono K (1987) The physical map of the wholeE. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50:495–508
Labedan B, Riley M (1995a) Widespread protein sequence similarities: origins ofEscherichia coli genes. J Bacteriol 177:1585–1588
Labedan B, Riley M (1995b) Gene products ofEscherichia coli: sequence comparisons and common ancestries. Mol Biol Evol (in press)
Mejean V, Iobbi-Nivol C, Lepelletier M, Giordano G, Chippaux M, Pascal MC (1994) TMAO anaerobic respiration inEscherichia coli; involvement of thetor operon. Mol Microbiol 11:1169–1179
Mortlock RP (1984) Microorganisms as model systems for studying evolution. Plenum Press, New York
Pierson DE, Campbell A (1990) Cloning and nucleotide sequence ofbisC, the structural gene for biotin sulfoxide reductase inEscherichia coli. J Bacteriol 177:2194–2198
Plunkett G III, Burland V, Daniels DL, Blattner FR (1993) Analysis of theEscherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes. Nucleic Acids Res 21:3391–3398
Shanmugan KT, Stewart V, Gunsalus RP, Boxer DH, Cole JA, Chippaux M, De Moss JA, Giordano G, Lin EC, Rajagopalan KV (1992) Proposed nomenclature for the genes involved in molybdenum metabolism inEscherichia coli andSalmonella typhmurium. Mol Microbiol 6:3452–3454
Sofia HJ, Burland V, Daniels DL, Plunkett G 3rd, Blattner FR (1994) Analysis of theEscherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes. Nucleic Acids Res 22:2576–2586
Wooton JC, Nicolson RE, Cook JM, Walters DE, Burke JF, Doyle WA, Bray RC (1991) Enzymes depending on the pterin molybdenum cofactor: sequence families, spectroscopic properties of molybdenum and possible cofactor-binding domains. Biochem Biophys Acta 1057:157–185
Zinoni F, Birkmann A, Stadtman TC, Böck A (1986) Nucleotide sequence and expression of the selenocysteine-containing polypeptide of formate dehydrogenase (formate-hydrogen-lyase-linked) fromEscherichia coli. Proc Natl Acad Sci (US) 83:4650–4654
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del Campillo Campbell, A., Campbell, A. Alternative gene for biotin sulfoxide reduction inEscherichia coli K-12. J Mol Evol 42, 85–90 (1996). https://doi.org/10.1007/BF02198832
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DOI: https://doi.org/10.1007/BF02198832