Abstract
Nitrogen-fixation (C2H2-reduction) was demonstrated in wheat root nodules (p-nodules) induced by 2,4-dichlorophenoxyacetate (2,4-D) and inoculated withA. brasilense. By lowering the O2 tension it was possible to distinguish the nitrogenase activity of bacteria located within thep-nodule of the wheat root system from that in the rhizosphere. Using cytological evidence, nitrogenase activity was attributed mainly to be coming from the bacteria within thep-nodule. It was also shown that the host plant was able to supply the necessary substrate required for the bacterial N2-fixation (C2H2-reduction) within thep-nodules.
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Tchan, Y.T., Zeman, A.M.M. & Kennedy, I.R. Nitrogen fixation inpara-nodules of wheat roots by introduced free-living diazotrophs. Plant Soil 137, 43–47 (1991). https://doi.org/10.1007/BF02187430
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DOI: https://doi.org/10.1007/BF02187430