Skip to main content
SpringerLink
Log in

Recent advances in microalgal bioscience in Japan, with special reference to utilization of biomass and metabolites: a review

  • Published:
Journal of Applied Phycology Aims and scope Submit manuscript

Abstract

Japan is one of leading countries in the utilization of and research on microalgae, and various findings have been obtained. Many papers, however, have been published in Japanese, which prevents the information spreading far and wide. The purpose of this review is to introduce recent advances in the utilization of microalgae as well as their basic research in Japan. The discussion covers practical applications ofChlorella andSpirulina biomass to health foods, food additives and feed supplements. The current use of microalgae as live feeds for larvae in aquaculture is also summarized. With respect to microalgal metabolites the present status of research is described with a greater emphasis on bioactive compounds, pigments and oils as potential drugs, coloring matters and biofuels, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aoyama K, Miyake M, Yamada J, Miyake J, Uemura I, Hoshino T, Asada Y (1996) Application of vector pKE4-9 carrying a strong promoter to the expression of foreign proteins inSynechococcus PCC7942. J. mar. Biotechnol. 4: 64–67.

    Google Scholar 

  • Belay A, Ota Y, Miyakawa K, Shimamatsu H (1993) Current knowledge on potential health benefits ofSpirulina. J. appl. Phycol. 5: 235–241.

    Google Scholar 

  • Borowitzka MA (1988a) Vitamins and fine chemicals from microalgae. In Borowtizka MA, Borowitzka LJ (eds), Micro-algal Biotechnology. Cambridge Univ. Press, Cambridge: 169–174.

    Google Scholar 

  • Borowitzka MA (1988b) Fats, oils and hydrocarbons. In Borowitzka MA, Borowitzka LJ (eds), Micro-algal Biotechnology. Cambridge Univ. Press, Cambridge: 257–287.

    Google Scholar 

  • Borowitzka MA (1995) Microalgae as sources of pharmaceuticals and other biologically active compounds. J. appl. Phycol. 7: 3–15.

    Google Scholar 

  • Cannell RJP, Kellam SJ, Owsianka AM, Walker JM (1988) Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Medica 56: 10–14.

    Google Scholar 

  • Cannell RJP, Kellan SJ, Owsianka AM, Walker JM (1989) Microalgae and cyanobacteria as a source of glycosidase inhibitors. J. gen. Microbiol. 133: 1701–1705.

    Google Scholar 

  • Ebina H (1975)Spirulina. Shoku no Kagaku 27: 57–65. (Japanese)

    Google Scholar 

  • Fukino H, Kamoashi T, Yamane Y, Miyagawa K, Kato T (1989) Effects ofSpirulina on nephrotoxicity due to inorganic mercury and cisplatin. Absts. Papers, the 109th Ann. Meetg. Jpn. Pharmacol. Soc. 630. (Japanese)

  • Fukui S (1979) Studies on amino acid metabolism of diabetics II. With special reference to the effects ofChlorella. Kiso to Rinsho 13: 289–300. (Japanese)

    Google Scholar 

  • Fukusho K (1981) Production of the rotiferBrachionus plicatilis. Yoshoku 18 (7): 44–51. (Japanese)

    Google Scholar 

  • Fukuyo Y (1994) Biotoxins. In Jpn. Soc. Fish. Sci. (ed.), Recent Advances in Fisheries Science. Koseisha-Koseikaku, Tokyo: 258–264. (Japanese)

    Google Scholar 

  • Furukawa K, Sakai K, Watanabe S, Maruyama K, Murakami M, Yamaguchi K, Ohizumi Y (1993) Goniodomin A induces modulation of actomyosin ATPase activity mediated through conformational change of actin. J. biol. Chem. 268: 26026–26031.

    PubMed  Google Scholar 

  • Guanzon NG, Nakamura H, Nishimura K (1995) Accumulation of copper, zinc, cadmium, and their combinations by three freshwater microalgae. Fisheries Sci. 61: 149–156.

    Google Scholar 

  • Hanagata N, Takeuchi T, Fukuju Y, Barnes DJ, Karube I (1992) Tolerance of microalgae to high CO2 and high temperature. Phytochemistry 31: 3345–3348.

    Google Scholar 

  • Hashimoto Y (1979) Marine Toxins and Other Bioactive Marine Metabolites. Jpn. Sci. Soc. Press, Tokyo, 369 pp.

    Google Scholar 

  • Hasuda S, Mito Y (1966) An experimental use ofChlorella for a patient with incurable wound. Shindan to Shinyaku 3: 17–20. (Japanese)

    Google Scholar 

  • Hasui M, Matsuda M, Yoshimatsu S, Okutani K (1995) Production of a lactate-associated galactan sulfate by a dinoflagellateGymnodinium A3. Fisheries Sci. 61: 363–364.

    Google Scholar 

  • Hayashi T, Suitani Y, Murakami M, Yamaguchi K, Konosu S, Noda H (1986) Protein and amino acid compositions of five species of marine phytoplankton. Bull. Jpn. Soc. Sci. Fish. 52: 337–343.

    Google Scholar 

  • Hirano M, Imai M, Abe K, Manabe E (1995) Influence of photosynthetic activity on fatty acid production bySpirulina platensis. J. mar. Biotechnol. 3: 86–88.

    Google Scholar 

  • Hirano M, Mori H, Miura Y, Matsunaga N, Nakamura N, Matsunaga T (1990) γ-Linolenic acid production by microalgae. Appl. Biochem. Biotechnol. 24/25: 183–191.

    Google Scholar 

  • Hirayama K, Maruyama I, Maeda T (1989) Nutritional effect of freshwaterChlorella on growth of the rotiferBranchionus plicatilis. Hydrobiologia 186/187: 39–42.

    Google Scholar 

  • Honjo T, Asakawa M (1990) Allelopathic substances. In Yasumoto T (ed.), Bioactive Metabolites of Marine Microorganisms. Koseisha-Koseikaku, Tokyo: 41–53. (Japanese)

    Google Scholar 

  • Hosotani K, Kitaoka S (1977) Determination of the nutritive value ofEuglena gracilis protein byin vitro digestion experiments and rat feeding tests. Nippon Nogeikagaku Kaishi 51: 483–488. (Japanese, with English summary)

    Google Scholar 

  • Hosoyamada Y, Takai Y, Kato T (1991) Effects of water-soluble and insoluble fractions ofSpirulina on serum lipids and glucose tolerance of rats. J. Jpn. Soc. Nutr. Food Sci. 44: 273–277. (Japanese, with English summary)

    Google Scholar 

  • Igarashi T, Yasumoto T (1996) Structure determination of the principle of mysterious red-tide toxin, prymnesin. Kagaku to Seibutsu 34: 495–497. (Japanese)

    Google Scholar 

  • Igarashi T, Satake M, Yasumoto T (1996) Prymnesin-2: A potent ichthyotoxic and hemolytic glycoside isolated from the red tide algaPrymnesium parvum. J. Am. chem. Soc. 118: 479–480.

    Google Scholar 

  • Ikemoto H, Shimada A, Maruyama T, Miyachi S (1995) Novel microalgae from the ocean. J. mar. Biotechnol. 3: 9–15.

    Google Scholar 

  • Imada M (1978) Red sea bream and crustaceans. In Jpn. Soc. Fish. Sci. (ed.), Carotenoids of Aquatic Animals. Koseisha-Koseikaku, Tokyo: 108–122. (Japanese)

    Google Scholar 

  • Ishida K, Murakami M, Matsuda H, Yamaguchi K (1995) Micropeptin 90, a plasmin and trypsin inhibitor from the bluegreen algaMicrocystis aeruginosa (NIES-90). Tetrahedron Lett. 36: 3535–3538.

    Google Scholar 

  • Ishida Y (1990) Substances controlling growth and behavior. In Yasumoto T (ed.), Bioactive Metabolites of Marine Microorganisms. Koseisha-Koseikaku, Tokyo: 30–40. (Japanese)

    Google Scholar 

  • Ito Y, Ito S, Kanamura H, Masaki K (1987) Dietary effect of attaching diatomNavicula ramosissima on mass production of young sea urchinPseudocentrotus depressus. Nippon Suisan Gakkaishi 53: 1753–1740. (Japanese, with English summary).

    Google Scholar 

  • Iwamoto H (1975)Spirulina; Hope for a new protein source. Kagaku to Kogyo 28: 110–113. (Japanese)

    Google Scholar 

  • Iwamoto H (1986) Hydrocarbon production by microalgae. Hakko to Kogyo 44: 1160–1167. (Japanese)

    Google Scholar 

  • Iwamoto H (1989) Cultivation ofBotryococcus braunii and hydrocarbon production. In Miyachi S, Karube I, Ishida Y (eds), Current Topics in Marine Biotechnology. Jpn. Soc. Mar. Biotechnol. Tokyo: 123–126.

    Google Scholar 

  • Iwamoto H (1990) Petroleum made by microalgae. Nenryo Kyokaishi 69: 230–238. (Japanese, with English summary)

    Google Scholar 

  • Iwata T, Inayama T, Kato T (1987a) Effects ofSpirulina platensis on fructose-induced hyperlipidemia in rats. J. Jpn. Soc. Nutr. Food Sci. 40: 463–467. (Japanese, with English summary)

    Google Scholar 

  • Iwata T, Inayama T, Miwa S, Kato T (1987b) Effects ofSpirulina platensis on blood lipase activity of fructose-induced hyperlipidemia Wister rats. Bull. Kagawa Nutr. Univ. 18: 53–58. (Japanese)

    Google Scholar 

  • Iwata T, Munekata H, Inayama T, Kato T (1990) Effects ofSpirulina platensis on the blood pressure of rats. Bull. Kagawa Nutr. Univ. 21: 63–70. (Japanese)

    Google Scholar 

  • Jpn. Bioind. Assoc. (ed.) (1983) Investigation on the Production of Fuels by Oil Plants: Comprehensive Survey on Microalgae. Jpn. Bioind. Assoc., Tokyo, 228 pp. (Japanese)

  • Jpn. Bioind. Assoc. (ed.) (1984) Investigation on the Production of Fuels by Oil Plants: Comprehensive Survey on Microalgae. Jpn. Bioind. Assoc., Tokyo, 209 pp. (Japanese)

  • Jpn. Bioind. Assoc. (ed.), (1985) Feasibility Studies on Techniques in Production and Utilization of New Fuel Oils by Using Biotechnology: Investigation on Production System of Fuel Oils by Microalgae. Jpn. Bioind. Assoc., Tokyo, 101 pp. (Japanese)

  • Jpn. Bioind. Assoc. (ed.) (1986) Feasibility Studies on Techniques in Production and Utilization of New Fuel Oils by Using Biotechnology: Investigation on Production System of Fuel Oils by Microalgae. Jpn. Bioind. Assoc., Tokyo, 126 pp. (Japanese)

  • Jpn. Bioind. Assoc. (ed.) (1987) Feasibility Studies on Techniques in Production and Utilization of New Fuel Oils by Using Biotechnology: Investigation on Production System of Fuel Oils by Microalgae. Jpn. Bioind. Assoc., Tokyo, 140 pp. (Japanese)

  • Jpn. Soc. Fish. Sci. (ed.) (1982) Toxic Phytoplankton: Occurrence, Mode of Action and Toxins. Koseisha-Koseikaku, Tokyo, 135 pp. (Japanese)

  • Kadota H (ed.) (1987) Freshwater Red Tides. Koseisha-Koseikaku, Tokyo, 304 pp. (Japanese)

    Google Scholar 

  • Kageyama H, Ishii A, Matsuoka T, Kodera Y, Hiroto M, Matsushima A, Inada Y (1994) Simple isolation of phycocyanin fromSpirulina platensis and phycocyanobilin-protein interaction. J. mar. Biotechnol. 1: 185–188.

    Google Scholar 

  • Kakisawa H (1990) Biologically active substances from aquatic microorganisms. In Yasumoto T (ed.), Bioactive Metabolites of Marine Microorganisms. Koseisha-Koseikaku, Tokyo: 54–64. (Japanese)

    Google Scholar 

  • Kamoashi T, Fukino H, Yamane Y, Kato T, Shimamatsu H (1988) Effects ofSpirulina on alleviation of neophrotoxicity due to inorganic mercury and p-aminophenol. Absts. Papers, the 108th Ann. Meetg. Jpn. Pharmcol. Soc. 58. (Japanese)

  • Kapoor M, Wakasugi T, Yoshinaga K, Sugiura M (1996) The chloroplast chlL gene of the green algaChlorella vulgaris C-27 contains a self splicing group I intron. Mol. gen. Genetics 250: 655–664.

    Google Scholar 

  • Kato T (1985) Present state and future directions in the utilization of pigments produced by microorganisms. Food. Chem. (8): 40–46. (Japanese)

    Google Scholar 

  • Kato T (1991) Chemistry of microalgae and their application to food. Food Chem. (8): 30–35. (Japanese)

    Google Scholar 

  • Kato T (1992)Spirulina. In Yamaguchi K (ed.), Utilization of Microalgae. Koseisha-Koseikaku, Tokyo: 32–44. (Japanese)

    Google Scholar 

  • Kato T, Takemoto K, Katayama H, Kuwabara Y (1984) Effects ofSpirulina platensis on dietary hypercholesterolemia in rats. J. Jpn. Soc. Nutr. Food Sci. 37: 323–332. (Japanese, with English summary)

    Google Scholar 

  • Kawamata M (1988) Studies on Extractive Nitrogenous Components of Microalgae. Ph.D. Disseration, Univ. Tokyo, 88 pp. (Japanese)

  • Kawamata M, Murakami M, Yamaguchi K, Konosu S (1988) Extractive nitrogenous components of the blue-green algaSpirulina maxima. Nippon Suisan Gakkaishi 54: 1453.

    Google Scholar 

  • Kawamura S (1977) Introduction of researches onSpirulina in France. Ferment. & Ind. 35: 854–860. (Japanese)

    Google Scholar 

  • Kayane H (1991) Coral reef eco-factories for fixing CO2. Mar. Biotechnol. Kenkyu Kaiho 4 (3): 14–27. (Japanese)

    Google Scholar 

  • Kiffe M, Nokihara K, Matsunaga T (1995) Purification of docosahexaenoic acid (DHA) produced by marine microalgaIsochrysis galbana. J. mar. Biotechnol. 2: 139–142.

    Google Scholar 

  • Kikuchi M, Hirano A, Kunito S, Kawakami Y (1995) Fucoxanthin, an anti-oxidative substance from marine diatomPhaeodactylum tricornutum. J. mar. Biotechnol. 3: 132–135.

    Google Scholar 

  • Kitagawa I, Kobayashi M, Hayashi K, Kawazoe K (1989) Heterosigma-glycolipids I, II and III from a raphidophycean flagellateHeterosigma akashio. In Miyachi S, Karube I, Ishida Y (eds), Current Topics in Marine Biotechnology. Jpn. Soc. Mar. Biotechnol., Tokyo: 195–198.

    Google Scholar 

  • Kitajima C (1983) The present status of marine fish seed production techniques in Japan. Proc. Symp. Int. Aquaculture, Coquimbo: 375–390.

  • Kitajima Y, Hagiwara K, Koshikawa Y, Sakurai N (1992) Microbial modification of exterior wall appearance using microalgae. Ann. Rep. Kajima Tech. Res. Inst. 40: 329–334. (Japanese, with English summary).

    Google Scholar 

  • Kitaoka S (ed.) (1989)Euglena: Physiology and Biochemistry. Jpn. Sci. Soc. Press, Tokyo, 272 pp. (Japanese)

    Google Scholar 

  • Kitaoka S (1993) Culture, chemistry and utilization ofEuglena. In Oishi K (ed.), Science of Algae. Asakura Shoten, Tokyo: 108–119. (Japanese)

    Google Scholar 

  • Kitazato H, Asaoka S, Iwamoto H (1989) Catalytic cracking of hydrocarbons from microalgae. Sekiyu Gakkaishi 32: 28–34. (Japanese, with English summary)

    Google Scholar 

  • Kobayashi J (1988) Noticeable bioactive substances in symbiotic association with algae: A new trend in the research on marine bioactive substances. Gendai Kagaku (12): 24–29. (Japanese)

    Google Scholar 

  • Kobayashi J, Yamaguchi N, Ishibashi M (1994a) Amphidinolide M, a novel 29-membered macrolide from the cultured marine dinoflagellateAmphidinium sp. J. org. Chem. 59: 4698–4700.

    Google Scholar 

  • Kobayashi J, Yamaguchi N, Ishibashi M (1994b) Amphidinin A, a novel amphidinolide-related metabolite from the cultured marine dinoflagellateAmphidinium sp. Tetrahedron Lett. 35: 7049–7050.

    Google Scholar 

  • Kobayashi M, Kakizono T, Nagai S (1991) Astaxanthin production by a green alga,Haematococcus pluvialis accompanied with morphological changes in acetate media. J. Ferment. Bioengng. 71: 335–339.

    Google Scholar 

  • Kodama M, Kurano N (1992) Growth of microalgae under high concentration of CO2: Search for a microalgal strain useful for the treatment of industrial waste gases. MBI Report: 57–60.

  • Kodama M, Ikomoto H, Miyachi S (1993) A new species of highly CO2-tolerant fast-growing marine microalga suitable for high-density culture. J. mar. Biotechnol. 1: 21–25. Kogyo Kaihatsu Kenkyusho (ed.) (1983) Investigation on the Production of Fuels by Oil Plants. Kogyo Kaihatsu Kenkyusho, Tokyo, 198 pp. (Japanese)

    Google Scholar 

  • Kogyo Kaihatsu Kenkyusho (ed.) (1984) Investigation on the Production of Fuels by Oil Plants: Survey on Mass Production System of Fuels. Kogyo Kaihatsu Kenkyusho, Tokyo, 253 pp. (Japanese)

    Google Scholar 

  • Komai Y, Onuki K, Yamagishi H, Shiratori T (1978) Photosensitive dermatitis due toChlorella tablets. Food Sanit. Res. 28: 747–752. (Japanese)

    Google Scholar 

  • Konishi F, Tanaka K, Himeno K, Taniguchi K, Nomoto K (1985) Antitumor effect induced by a hot water extract ofChlorella vulgaris: Resistance to Meth-A tumor growth mediated by CE-induced polymorphonuclear leukocytes. Cancer Immunol. Immunother. 19: 73–78.

    PubMed  Google Scholar 

  • Kozakai H, Oshima Y, Yasumoto T (1982) Isolation and structural elucidation of hemolysin from the phytoflagellatePrymnesium parvum. Agric. biol. Chem. 46: 233–236.

    Google Scholar 

  • Kumazawa S (1991) Hydrogen production by blue-green algae. Mar. Biotechnol. Kenkyu Kaiho 4 (2): 18–27. (Japanese)

    Google Scholar 

  • Kumazawa S, Shimamura K (1993) Photosynthesis-dependent production of H2 by a marine N2-fixing cyanobacterium,Anabaena sp. TU37-1. J. mar. Biotechnol. 1: 159–162.

    Google Scholar 

  • Kurano N, Ikemoto H, Miyashita H, Hasegawa T, Miyachi S (1995a) Carbon dioxide uptake rate ofChlorococcus littorale. J. mar. Biotechnol. 3: 108–110.

    Google Scholar 

  • Kurano N, Ikemoto H, Miyashita H, Hasegawa T, Hata H, Miyachi S (1995b) Fixation and utilization of carbon dioxide by microalgal photosynthesis. Energy Convers. Mgmt 36: 689–692.

    Google Scholar 

  • Kuwada Y, Ohta Y (1987) Hydrogen production by an immobilized cyanobacteriumLyngbya sp. J. Ferment. Technol. 65: 597–602.

    Google Scholar 

  • Liao WL (1990) Studies on the Improvement of Flesh Quality of Cultured Striped JackPseudocaranx dentex. Ph.D. Dissertation, Univ. of Tokyo, Tokyo, 96 pp. (Japanese)

    Google Scholar 

  • Liao WL, Takeuchi T, Watanabe T, Yamaguchi K (1990) Effect of dietarySpirulina supplementation on extractive nitrogenous constituents and sensory test of cultured striped jack flesh. J. Tokyo Univ. Fish. 77: 241–246. (Japanese, with English summary)

    Google Scholar 

  • Liao WL, Nur-E-Borhan SA, Okada S, Matsui T, Yamaguchi K (1993) Pigmentation of cultured black tiger prawn by feeding with aSpirulina-supplemented diet. Nippon Suisan Gakkaishi 59: 165–169.

    Google Scholar 

  • Lincoln RA, Strupinski K, Walker JM (1991) Enzyme inhibitors from algae. Biochem. Soc. Trans. 19: 429S.

  • Manabe E, Hirano M, Takano H, Ishikawa-Doi N, Sode K, Matsunaga T (1992) Influence of ammonium chloride on growth and fatty acid production bySpirulina platensis. Appl. Biochem. Biotechnol. 34/35: 273–281.

    Google Scholar 

  • Maruyama I, Ando Y (1992)Chlorella. In Yamaguchi K (ed.), Utilization of Microalgae. Koseisha-Koseikaku, Tokyo: 18–31. (Japanese)

    Google Scholar 

  • Maruyama I, Ando Y, Maeda T, Hirayama K (1989) Uptake of vitamin B12 by various strains of unicellular algaeChlorella. Nippon Suisan Gakkaishi 55: 1785–1790.

    Google Scholar 

  • Maruyama I, Kanamaru H, Nakamura N, Ando Y, Hirayama K (1990) Open culture of the rotiferBrachionus plicatilis by feeding vitamin B12-containingChlorella. Suisan Zoshoku 38: 227–231. (Japanese)

    Google Scholar 

  • Matsumoto H, Hamazaki A (1992) Utilization in space. In Yamaguchi K (ed.), Utilization of Microalgae. Koseisha-Koseikaku, Tokyo: 116–127. (Japanese)

    Google Scholar 

  • Matsunaga T (1990) Biotechnology of marine photosynthetic procaryote. Kagaku to Seibutsu 27: 513–520. (Japanese)

    Google Scholar 

  • Matsunaga T (1992) Development of biotechnology. In Yamaguchi K (ed.), Utilization of Microalgae. Koseisha-Koseikaku, Tokyo: 89–101. (Japanese)

    Google Scholar 

  • Matsunaga T, Sudo H, Takemasa Y, Wachi Y, Nakamura N (1996) Sulfated extracellular polysaccharide production by the halophilic cyanobacteriumAphanocapsa halophyta immobilized on light-diffusing optical fibers. Appl. Microbiol. Biotechnol. 45: 24–27.

    Google Scholar 

  • Matsuno T (1994) Carotenoids. In Jpn. Soc. Fish. Sci. (ed.), Recent Advances in Fisheries Science. Koseisha-Koseikaku, Tokyo: 292–306. (Japanese)

    Google Scholar 

  • Matsuno T, Katsuyama M, Iwahashi M, Koike T, Okada M (1980) Intensification of color of redTilapia with lutein, rhodoxanthin andSpirulina. Bull. Jpn. Soc. Sci. Fish. 46: 479–482. (Japanese, with English summary)

    Google Scholar 

  • Matsuno T, Nagata S, Iwahashi M, Koike T, Okada M (1979) Intensification of color of fancy red carp with zeaxanthin and myxoxanthophyll, major carotenoid constituents ofSpirulina. Bull. Jpn. Soc. Sci. Fish. 45: 627–632. (Japanese, with English summary)

    Google Scholar 

  • Miki W, Yamaguchi K, Konosu S (1986) Carotenoid composition ofSpirulina maxima. Bull. Jpn. Soc. Sci. Fish. 52: 1225–1227.

    Google Scholar 

  • Mitsuda H, Shikanai T, Yoshida K (1961) Studies on the utilization ofChlorella as a foodstuff (part 6). Growth stimulating factors inChlorella and Tolura yeast for lactic acid bacteria (I). Eiyo to Shokuryo 14: 28–34. (Japanese)

    Google Scholar 

  • Mitsuda H, Higuchi M, Yamamoto A, Nakjima K (1977) Protein concentrate from the blue-green algae and its nutritive value. Eiyo to Shokuryo 30: 23–28. (Japanese, with English summary)

    Google Scholar 

  • Miura Y, Matsunaga T (1989) Antibiotics production from marine microalgae. In Miyachi S, Karube I, Ishida Y (eds), Current Topics in Marine Biotechnology. Jpn. Soc. Mar. Biotechnol., Tokyo: 189–190.

    Google Scholar 

  • Miura Y, Sode K, Narasaki Y, Matsunaga T (1993) Light-induced anti-microbial activity of extracts from marineChlorella. J. mar. Biotechnol. 1: 143–146.

    Google Scholar 

  • Miyake M, Asada Y (1996) Efficient transformation of a thermophilic cyanobacterium,Synechococcus elongatus. J. mar. Biotechnol. 4: 113–116.

    Google Scholar 

  • Miyake M, Yamada J, Aoyama K, Uemura I, Hoshino T, Miyake J, Asada Y (1996) Strong expression of foreign protein inSynechococcus PPC7942. J. mar. Biotechnol. 4: 61–63.

    Google Scholar 

  • Miyakoshi M, Tanaka M, Miyazawa K, Nara H, Takemoto Y, Maki T, Fukui S, Yasutoku H, Arayasu K, Shimizu K (1980) Studies onChlorella IV. With special reference to hypertension. Kiso to Rinsho 14: 191–200. (Japanese)

    Google Scholar 

  • Miyamoto K (1995) The use of microalgae in bioremediation. Biosci. Bioind. 53: 1029–1035. (Japanese)

    Google Scholar 

  • Miyashita H, Kurano N, Miyachi S (1995) Composition and nature of extracellular polysaccharide produced by newly isolated coccoid prasinophyta,Prasinococcus capsulatus. J. mar. Biotechnol. 3: 136–139.

    Google Scholar 

  • Mori T, Muranaka T, Miki W, Yamaguchi K, Konosu S, Watanabe T (1987) Pigmentation of cultured sweet smelt fed diets supplemented with a blue-green algaSpirulina maxima. Nippon Suisan Gakkaishi 53: 433–438.

    Google Scholar 

  • Murakami M, Yamaguchi K (1989) Toxicity of red tides. Suishitsu Odaku Kenkyu 12: 757–762. (Japanese)

    Google Scholar 

  • Murakami M, Yamaguchi K (1990) Biologically active compounds of microalgae. In Yasumoto T (ed.), Bioactive Metabolites of Marine Microorganisms. Koseisha-Koseikaku, Tokyo: 73–85. (Japanese)

    Google Scholar 

  • Murakami M, Yamaguchi K (1992) Bioactive compounds produced by microalgae. In Yajima H, Shioiri T, Ohizumi Y (eds), The Second Series of Pharmaceutical Research and Development. Vol. 10, Marine Resources for Drug Discovery. Hirokawa Pub. Co., Tokyo: 363–377. (Japanese)

    Google Scholar 

  • Murakami M, Makabe K, Yamaguchi K, Konosu S (1989) Cytotoxic polyunsaturated fatty acid fromPediastrum. Phytochemistry 28: 625–626.

    Google Scholar 

  • Murakami M, Matsuda H, Makabe K, Yamaguchi K (1991) Oscillariolide, a novel macrolide from a blue-green algaOscillatoria sp. Tetrahedron Lett. 32: 2391–2394.

    Google Scholar 

  • Murakami M, Makabe K, Yamaguchi K, Konosu S, Walchli MR (1988a) Goniodomin A, a novel polyether macrolide from the dinoflagellateGoniodoma pseudogoniolaux. Tetrahedron Lett. 29: 1149–1152.

    Google Scholar 

  • Murakami M, Okita Y, Matsuda H, Okino T, Yamaguchi K (1994) Aeruginosin 298-A, a thrombin and trypsin inhibitor from the blue-green algaMicrocystis aeruginosa (NIES-298). Tetrahedron Lett. 35: 3129–3132.

    Google Scholar 

  • Murakami M, Ishida K, Okino T, Okita Y, Matsuda H, Yamaguchi K (1995) Aeruginosins 98-A and B, trypsin inhibitors from the blue-green algaMicrocystis aeruginosa (NIES-98). Tetrahedron Lett. 36: 2785–2788.

    Google Scholar 

  • Murakami M, Nakano H, Yamaguchi K, Konosu S, Nakayama O, Matsumoto Y, Iwamoto H (1988b) Meijicoccene, a new cyclic hydrocarbon fromBotryococcus braunii. Phytochemistry 27: 455–457.

    Google Scholar 

  • Murata M (1990) Novel macrolides from dinoflagellates. Kagaku to Seibutsu 27: 277–279. (Japanese)

    Google Scholar 

  • Murata M (1991) Polyether compounds from marine organisms. Nippon Nogeikagaku Kaishi 65: 1743–1751. (Japanese)

    Google Scholar 

  • Murata M, Tachibana K (1995) Ecofunctions of bioactive metabolites from dinoflagellates. Biosci. Bioind. 53: 883–885. (Japanese)

    Google Scholar 

  • Mustafa MG, Nakagawa H (1995) A review: Dietary benefits of algae as an additive in fish feed. Israeli J. Aquacul. 47: 163–172.

    Google Scholar 

  • Nakagawa H, Kasahara S, Tsujimura A, Akira K (1984) Changes of body composition during starvation inChlorella-extract fed ayu. Bull. Jpn. Soc. Sci. Fish. 50: 665–671.

    Google Scholar 

  • Nakagawa H, Kumai H, Nakamura M, Kasahara S (1982a) Effects ofChlorella-extract supplemented diet on cultured yellowtail II. Effects on resistability (when left standing in the air) from the viewpoint of hematological properties. Suisan Zoshoku 30: 76–83. (Japanese)

    Google Scholar 

  • Nakagawa H, Kasahara S, Uno E, Minami T, Akira K (1981) Effect of feeding ofChlorella-extract supplement in diet on resisting power of ayu. Suisan Zoshoku 29: 109–116. (Japanese)

    Google Scholar 

  • Nakagawa H, Inazuka Y, Yamazaki S, Hirata H, Kasahara S (1982b) Effects ofChlorella-extract supplemented diet on cultured yellowtail I. Effects on growth and hematological properties. Suisan Zoshoku 30: 65–75. (Japanese)

    Google Scholar 

  • Nakamura N, Nakamae H, Maeda N (1983) Effects ofChlorella extract on the growth of plants I. Effects on rooting and endogenous bioactive substances. Green Kenpo 45: 1–17. (Japanese)

    Google Scholar 

  • Nakamura N, Nakamae H, Maeda N (1984) Effects ofChlorella extract on the growth of plants II. Effects on endogenous auxin and ethylene formation. Green Kenpo 40: 1–7. (Japanese)

    Google Scholar 

  • Nakao H, Kuwazuka S (1991) Microbes in the soil and effects of hot water-extract ofChlorella on the growth of plants. Absts. Papers, Jpn. Soc. Soil & Fertilizer 37: 43. (Japanese)

    Google Scholar 

  • Nakaya N, Honma Y, Goto Y (1988) Cholesterol lowering effect ofSpirulina. Nutr. Rep. Int. 37: 1329–1337.

    Google Scholar 

  • Nakayama S (1975)Spirulina; a possibility of anew protein source. New Food Ind. 17: 7–13. (Japanese)

    Google Scholar 

  • Nanba T, Yoshimoto H, Kimura H (1986) Outline of feed culture from 1979 to 1984. Bull. Wakayama Saibai Cen. 1: 37–39. (Japanese)

    Google Scholar 

  • Negoro M, Shioji N, Ikuta Y, Makita T, Uchiumi M (1992) Growth characteristics of microalgae in high-concentration CO2 gas, effects of culture medium trace components, and impurities thereon. Appl. Biochem. Biotech. 34–5: 681–692.

    Google Scholar 

  • Nematipour GR, Nakagawa H, Kasahara S, Ohya S (1988) Effects of dietary lipid level andChlorella-extract on ayu. Nippon Suisan Gakkaishi 54: 1395–1400.

    Google Scholar 

  • Nematipour GR, Nakagawa H, Nanba K, Kasahara S, Tsujimura A, Akira K (1987) Effect ofChlorella-extract supplement to diet on lipid accumulation of ayu. Nippon Suisan Gakkaishi 53: 1687–1692.

    Google Scholar 

  • Nishino H (1993) Anticarcinogenic activity of carotenoids in foodstuffs. Nippon Nogeikagaku Kaishi 67: 39–41. (Japanese)

    Google Scholar 

  • Ochiai H (1991) Gene transfer into cyanobacteria. Biosci. Bioind. 49: 749–751. (Japanese)

    Google Scholar 

  • Oda T, Ishimatsu A (1995) Production of active oxygen and mechanism of toxicity development by red-tide plankton,Chattonella. Mar. Biotechnol. Kenkyu Kaiho 8 (1): 15–28. (Japanese)

    Google Scholar 

  • Ogawa R (1974) Technology on the production ofSpirulina and its utilization for foods. Shokuhin Kogyo 17 (4): 48–52. (Japanese)

    Google Scholar 

  • Okada S, Yamaguchi K (1989) Effects of nitrogen deficiency on pigment composition of the green algaBotryococcus braunii Berkeley. In Miyachi S, Karube I, Ishida Y (eds), Current Topics in Marine Biotechnology. Jpn. Soc. mar. Biotechnol., Tokyo: 119–122.

  • Okada S, Murakami M, Yamaguchi K (1995) Hydrocarbon composition of newly isolated strains of the green microalgaBotryococcus braunii. J. appl. Phycol. 7: 555–559.

    Google Scholar 

  • Okada S, Matsuda H, Murakami M, Yamaguchi K (1996) Botryoxanthin A, a member of a new class of carotenoids from the green microalgaBotryococcus braunii Berkeley. Tetrahedron Lett. 37: 1065–1068.

    Google Scholar 

  • Okada S, Liao WL, Mori T, Yamaguchi K, Watanabe T (1991) Pigmentation of cultured striped jack reared on diets supplemented with the blue-green algaSpirulina maxima. Nippon Suisan Gakkaishi 57: 1403–1406.

    Google Scholar 

  • Okaichi T (1974) Significance of amino acid composition of phytoplankton and suspensoid in marine biological production. Bull. Jpn. Soc. Sci. Fish. 40: 471–478.

    Google Scholar 

  • Okaichi T. Anderson DM, Nemoto T (eds) (1989) Red Tides — Biology, Environmental Science and Toxicology. Elsevier, New York, 49 pp.

    Google Scholar 

  • Okauchi M (1992) Live feeds for aquaculture. In Yamaguchi K (ed.), Utilization of Microalgae. Koseisha-Koseikaku, Tokyo: 75–88. (Japanese)

    Google Scholar 

  • Okauchi M, Zhou WJ, Zhou WH, Fukusho K, Kanazawa A (1990) Difference in nutritive value of a microalgaNannochloropsis oculata at various growth phases. Nippon Suisan Gakkaishi 58: 1293–1298. (Japanese, with English summary)

    Google Scholar 

  • Okino T, Matsuda H, Murakami M, Yamaguchi K (1993a) Microginin, an angiotensin-converting enzyme inhibitor from the blue-green algaMicrocystis aeruginosa. Tetrahedron Lett. 34: 501–504.

    Google Scholar 

  • Okino T, Matsuda H, Murakami M, Yamaguchi K (1995) New microviridins, elastase inhibitors from the blue-green algaMicrocystis aeruginosa. Tetrahedron 51: 10679–10686.

    Google Scholar 

  • Okino T, Murakami M, Haraguchi R, Munekata H, Matsuda H, Yamaguchi K (1993b) Micropeptins A and B, plasmin and trypsin inhibitors from the blue-green algaMicrocystis aeruginosa. Tetrahedron Lett. 34: 8131–8134.

    Google Scholar 

  • Ono K, Miyatake K, Inui H, Kitaoka S, Nakano Y (1995) Wax ester production by anaerobicEuglena gracilis. J. mar. Biotechnol. 2: 157–161.

    Google Scholar 

  • Oshima Y (1989) Worldwide outbreak of novel paralytic shellfish poisoning: causalty by the dinoflagellateGymnodinium catenatum. Kagaku to Seibutsu 26: 805–806. (Japanese)

    Google Scholar 

  • Oswald WJ (1988) Micro-algae and waste-water treatment. In Borowitzka MA, Borowitzka LJ (eds), Micro-algal Biotechnology. Cambridge Univ. Press, Cambridge: 305–328.

    Google Scholar 

  • Rinehart KL, Namikoshi M, Choi BW (1994) Structure and biosynthesis of toxins from blue-green algae (cyanobacteria). J. appl. Phycol. 6: 159–176.

    Google Scholar 

  • Saito T, Saito A, Oka N (1966) Clinical applications ofChlorella dosage. Shindan to Shinyaku 3: 61–64. (Japanese)

    Google Scholar 

  • Sano T (1993) Culture, chemistry and utilization ofChlorella. In Oishi K (ed.), Science of Algae. Asakura Shoten, Tokyo: 100–108. (Japanese)

    Google Scholar 

  • Sano T, Kaya K (1995) Oscillamide Y, a chymotrypsin inhibitor from toxicOscillatoria agardhii. Tetrahedron Lett. 36: 5933–5936.

    Google Scholar 

  • Sano T, Tanaka Y (1987) Effect of dried, powderedChlorella vulgaris on experimental athersclerosis and alimentary hypercholesterolemia in cholesterol-fed rabbits. Artery 14: 76–84.

    PubMed  Google Scholar 

  • Santillan C (1982) Mass production ofSpirulina. Experientia 38: 40–43.

    Google Scholar 

  • Sato M, Yoshinaka R, Morimoto H, Kuroshima R, Matuoka Y, Yanagawa T, Ikeda S (1984) Nutritive assessment ofEuglena as food for fish at early stage II. Nutritive value ofEuglena diet for rainbow trout fingerlings. Suisan Zoshoku 32: 88–91. (Japanese)

    Google Scholar 

  • Sawayama S, Inoue S, Yokoyama S (1994) Continuous culture of hydrocarbon-rich microalgaBotryococcus braunii in secondarily treated sewage. Appl. Microbiol. Biotechnol. 41: 729–733.

    Google Scholar 

  • Sawayama S, Minowa T, Dote Y, Yokoyama S (1992) Growth of the hydrocarbon-rich microalgaeBotryococcus braunii in secondarily treated sewage. Appl. Microbiol. Biotechnol. 38: 135–138.

    Google Scholar 

  • Scott JM (1981) The vitamin B12 requirement of marine rotiferBrachionus plicatilis. J. mar. biol. Ass. U.K. 61: 983–994.

    Google Scholar 

  • Sekino K, Shiraiwa Y (1995) CO2 release from the culture of calcifying and noncalcifying marine unicellular algae. J. mar. Biotechnol. 3: 101–104.

    Google Scholar 

  • Shigeoka S, Nakano Y (1989) Physiological functions ofEuglena organelle. Iden 40 (4): 60–65. (Japanese)

    Google Scholar 

  • Shin HJ, Matsuda H, Murakami M, Yamaguchi K (1996a) Agardhipeptins A and B, two new cyclic hepta- and octapeptides from the cyanobacteriumOscillatoria agardhii (NIES-204). Tetrahedron (in press).

  • Shin HJ, Murakami M, Matsuda H, Yamaguchi K (1996b) Microviridins D–F, serine protease inhibitors from the cyanobacteriumOscillatoria agardhii (NIES-204). Tetrahedron 52: 8159–8168.

    Google Scholar 

  • Shin HJ, Murakami M, Matsuda H, Ishida K, Yamaguchi K (1995) Oscillapeptin, an elastase and chymotrypsin inhibitor from the cyanobacteriumOscillatoria agardhii (NIES-204). Tetrahedron Lett. 36: 5235–5238.

    Google Scholar 

  • Shiomi K (1994) Natural toxins. In Jpn. Soc. Fish. Sci. (ed.), Recent Advances in Fisheries Science. Koseisha-Koseikaku, Tokyo: 375–379. (Japanese)

    Google Scholar 

  • Shirai M, Ohtake A, Sano T, Matsumoto S, Sakamoto T, Sato A, Aida T, Harada K, Shimada T, Suzuki M, Nakano M (1991) Toxicity and toxins of natural blooms and isolated strains ofMicrocystis spp. (Cyanobacteria) and improved procedure for purification of cultures. Appl. environ. Microbiol. 57: 1241–1245.

    PubMed  Google Scholar 

  • Sode K, Hatano N, Tatara M (1996a) Cloning of a marine cyanobacterial promoter for foreign gene expression using a promoter probe vector. Appl. Biochem. Biotech. 59: 349–355.

    Google Scholar 

  • Sode K, Oozeki M, Asakawa K, Burgess JG, Matsunaga T (1994) Isolation of a marine cyanophage infecting the marine unicellular cyanobacterium,Synchococcus sp. NKBG 042902. J. mar. Biotechnol. 1: 189–192.

    Google Scholar 

  • Sode K, Hayashi T, Tatara M, Hatano N, Yoshida H, Takeyama H, Oshiro T, Matsunaga T (1996b) Recovery of a marine cyanobacterial recombinant product using fish-feed organisms. J. mar. Biotechnol. 4: 82–86.

    Google Scholar 

  • Sonoda M (1972) Effect ofChlorella extract on pregnancy anemia. Jpn. J. Nutrition 30: 218–225. (Japanese)

    Google Scholar 

  • Sonoda M, Okuda M (1978) Effect ofChlorella on neurosis at puberty. Shindan to Shinyaku 15: 85–88. (Japanese)

    Google Scholar 

  • Sue S, Sugiya K, Furuki M, Shimizu T, Inoue Y, Nakamoto H, Hiyama T (1995) Nucleotide sequence of the psaD gene from thermophilic cyanobacteriumSynechococcus vulcanus. Photosynth. Res. 46: 265–268.

    Google Scholar 

  • Suzuki O (1987) Research and development in manufacturing techniques of microbial essential fatty acids. Bidec News (51): 12–15. (Japanese)

    Google Scholar 

  • Takagi Y, Fukino H, Yamane Y (1990) Effects ofSpirulina on acute nephrotoxicity. Abstr. Papers, the 110th Ann. Meetg. Jpn. Pharmacol. Soc. 209. (Japanese)

  • Takano H, Takai R, Manabe E, Matsunaga T (1995) Production of coccolith particle by coccolithophorid algaEmiliania huxleyi. J. mar. Biotechnol. 3: 93–96.

    Google Scholar 

  • Takano H, Jeon J, Burgess JG, Manabe E, Izumi Y, Okazaki M, Matsunaga T (1994) Continuous production of extracellular ultrafine calcite particles by the marine coccolithophorid algaPleurochrysis carterae. Appl. Microbiol. Biotechnol. 40: 946–950.

    Google Scholar 

  • Takenaka H, Yamaguchi Y, Teramoto S, Tanaka N, Hori M, Seki H, Hiwatari T (1996a) Evaluation of the mutagenic properties of the coccolithophorePleurochrysis carterae (Haptophyceae) as a potential human food supplement. J. appl. Phycol. 8: 1–3.

    Google Scholar 

  • Takenaka H, Yamaguchi Y, Teramoto S, Tanaka N, Hori M, Seki H, Nishimori T, Morinaga T (1996b) Safety evaluation ofPleurochrysis carterae as a potential food supplement. J. mar. Biotechnol. 3: 274–277.

    Google Scholar 

  • Takeshima Y (1996) Efficient expression of foreign genes in cyanobacteria. Biosci. Bioind. 54: 100–104. (Japanese)

    Google Scholar 

  • Takeshima Y, Hagiwara H (1991) Expression of foreign genes in cyanobacteria. Biosci. Bioind. 49: 1263–1268. (Japanese)

    Google Scholar 

  • Takeuchi Y, Yoneyama K, Konnai M, Takematsu T (1987) Physiological effects of a water extract ofChlorella vulgaris on the growth of plants. J. Jpn. Soc. Turfgrass Sci. 15: 11–16.

    Google Scholar 

  • Takeyama H, Nakamura N, Matsunaga T (1989) Transformation of marine cyanobacteriumSynechococcus sp. by electroporation. In Miyachi S, Karube I, Ishida Y (eds), Current Topics in Marine Biotechnology. Jpn. Soc. Mar. Biotechnol., Tokyo: 159–160.

    Google Scholar 

  • Takeyama H, Iwamoto K, Hata S, Takano H, Matsunaga T (1996) DHA enrichment of rotifers: A simple two-step culture using the unicellular algaeChlorella regularis andIsochrysis galbana. J. mar. Biotechnol. 3: 244–247.

    Google Scholar 

  • Tamura Y, Nishigaki S, Maki T, Shimamura Y, Noi Y (1978) Biological chemical examination onChlorella tablets. Food Sanit. Res. 28: 753–759. (Japanese)

    Google Scholar 

  • Tanabe Y (1979) Phycocyanin. New Food Ind. 21 (2): 43–46. (Japanese)

    Google Scholar 

  • Tanaka K, Konishi F, Himeno K, Taniguchi K, Nomoto K (1984) Augmentation of antitumor resistance by a strain of unicellular green algaChlorella vulgaris. Cancer Immunol. Immunother. 17: 90–94.

    PubMed  Google Scholar 

  • Tanaka N (1991) Large-scale culture system for benthic microalgae. NOAA Tech. Rep. NMFS 102: 83–88.

    Google Scholar 

  • Tani Y, Okumura M, Ii S (1987) Liquid wax ester production byEuglena gracilis. Agric. biol. Chem. 51: 225–230.

    Google Scholar 

  • Tatsuzawa H, Takizawa E (1995) Changes in fatty acid composition ofPavlova lutheri (Prymnesiophyceae) affected by culturing conditions. Fisheries Sci. 61: 363–364.

    Google Scholar 

  • Terao K, Ito E, Murakami M, Yamaguchi K (1989) Histopathological studies on experimental marine toxin poisoning — III. Morphological changes in the liver and thymus of male ICR mice induced by goniodomin A, isolated from the dinoflagellateGoniodoma pseudogoniaulax. Toxicon 27: 269–271.

    PubMed  Google Scholar 

  • Tokuda H, Nishino H, Shirahashi H, Murakami N, Nagatsu A, Sakakibara J (1996) Inhibition of 12-O-tetradecanoylphorbol-13-acetate promoted mouse skin papilloma by digalactosyl diacylglycerols from the freshwater cyanobacteriumPhormidium tenue. Cancer Lett. 104: 91–96.

    PubMed  Google Scholar 

  • Tokuyasu M (1983) Examples of diets for infants' and children's nutritional guidance and their effects. Jpn. J. Nutrn. 41: 273–283. (Japanese)

    Google Scholar 

  • Tsuchihashi N, Watanabe T, Takai Y (1987) Effects ofSpirulina on appendix contents of rats. Bull. Chiba Coll. Health Sci. 5 (2): 27–30. (Japanese)

    Google Scholar 

  • Tsuda M, Sasaki T, Kobayashi J (1994) Amphidinolide L, a new cytotoxic 27-membered macrolide from the cultured dinoflagellateAmphidinium sp. J. org. Chem. 59: 3734–3737.

    Google Scholar 

  • Uki E, Kikuchi S (1979) Food value of six benthic micro-algae on growth of juvenile abalone,Haliotis discuss hannai. Bull. Tohoku Fish. Res Lab. 40: 47–52. (Japanese, with English summary)

    Google Scholar 

  • Wachi Y, Burgess JG, Takahashi J, Nakamura N, Matsunaga T (1995) Tyrosinase inhibition by the water-soluble fraction of marine microalgae. J. mar. Biotechnol. 2: 210–213. Watanabe MF, Harada K, Carmichael WW, Fujiki H (eds) (1996) Toxic Microcystis. CRC Press Inc., Boca Raton, 262 pp.

    Google Scholar 

  • Watanabe T, Liao WL, Takeuchi T, Yamamoto H (1990) Effect of dietarySpirulina supplementation on growth performance and flesh lipids of cultured striped jack. J. Tokyo Univ. Fish. 77: 231–239. (Japanese, with English summary)

    Google Scholar 

  • Winder JS, Cannell RJP, Walker JM, Delbarre S, Francisco C, Farmer PB (1989) Glycosidase inhibitors from algae. Biochem. Soc. Trans. 17: 1030–1031.

    PubMed  Google Scholar 

  • Yamada K (1974) A review onSpirulina. J. Ferment. Assoc., Jpn. 32: 131–134. (Japanese)

    Google Scholar 

  • Yamagishi Y, Makawa M, Suzuki K, Hara T, Warita F (1962) Therapy for gastric ulcer withChlorella. Jpn. Med. J. 1997: 25–27. (Japanese)

  • Yamaguchi K (1989) Utilization of plankton — 7. Plankton as energy resources (3). Kaiyo to Seibutsu 11: 15–19. (Japanese)

    Google Scholar 

  • Yamaguchi K (1991) Utilization of plankton — 12. Plankton as fine chemical resources (3). Kaiyo to Seibutsu 13: 340–344. (Japanese)

    Google Scholar 

  • Yamaguchi K (ed.) (1992a) Utilization of Microalgae. Koseisha-Koseikaku, Tokyo, 132 pp. (Japanese)

    Google Scholar 

  • Yamaguchi K (1992b) Utilization of plankton — 14. Plankton as fine chemical resources (5). Kaiyo to Seibutsu 14: 431–433. (Japanese)

    Google Scholar 

  • Yamaguchi K (1992c) Chemistry and utilization of microalgae. BIO-medica 7: 269–274. (Japanese)

    Google Scholar 

  • Yamaguchi K, Murakami M, Okino T (1989) Screening of angiotensin-converting enzyme inhibitory activities in microalgae. J. appl. Phycol. 1: 271–275.

    Google Scholar 

  • Yamaguchi K, Kawamata M, Murakami M, Konosu S, Ben-Amotz A (1988) Extractive nitrogenous components of halotolerant algaDunaliella bardawil. Nippon Suisan Gakkaishi 54: 239–243.

    Google Scholar 

  • Yamaguchi K, Nakano H, Murakami M, Konosu S, Nakayama O, Kanda M, Nakamura A, Iwamoto H (1987b) Lipid composition of a green alga,Botryococcus braunii. Agric. biol. Chem. 51: 493–498.

    Google Scholar 

  • Yamaguchi K, Kato H, Murakami M, Watanabe K, Konosu S, Hirano T, Watanabe T, Yamamoto H, Yoshida N, Kitajima C (1987a) Effects of aSpirulina-supplemented diet on flesh quality, taste and flavor of cultured red sea bream. Absts. Papers, Ann. Meetg. Jpn. Soc. Fish. Sci. 45. (Japanese)

  • Yamaguchi M (1979) Problems in the utilization ofSpirulina. New Food Ind. 21: 34–41. (Japanese)

    Google Scholar 

  • Yamaoka Y (1995) Capture and absorption of polluting elements by the marine microalgaDunaliella. Mar. Biotechnol. Kenkyu Kaiho 8 (2): 3–15. (Japanese)

    Google Scholar 

  • Yamaoka Y, Takimura O, Fuse H, Kamimura K (1995) β-Carotene production byDunaliella salina in fed-batch and semi-continuous cultures under nutrient supplement. Seibutsu-Kogaku Kaishi 72: 111–114. (Japanese, with English summary)

    Google Scholar 

  • Yamaoka Y, Takimura O, Fuse H, Kamimura K, Manabe E, Takano H, Hirano M (1992) Effects of various environmental factors on β-carotene production byDunaliella salina. Hakko-Kogaku Kaishi 70: 25–28. (Japanese, with English summary)

    Google Scholar 

  • Yanagihashi S, Yanagisawa T, Kawasaki T (1984) Settlement of floating larvae in the seed production of sea cucumber and subsequent feeds and rearing method. Suisan Zoshoku 32: 6–14. (Japanese)

    Google Scholar 

  • Yasumoto T (1992) Bioactive polyethers of ciguatera and symbiotic algae. In Yajima H, Shioiri T, Ohizumi Y (eds), The Second Series of Pharmaceutical Research and Development. Vol. 10, Marine Resources for Drug Discovery. Hirokawa Publ. Co., Tokyo: 377–391. (Japanese)

    Google Scholar 

  • Yasumoto T (1993) Marine Toxins. Chem. Rev. 93: 1897–1909.

    Google Scholar 

  • Yasumoto T, Nagai H (1990) Antifungal substances from plankton. In Yasumoto T (ed.), Bioactive Metabolites of Marine Microorganisms. Koseisha-Koseikaku, Tokyo: 65–72. (Japanese)

    Google Scholar 

  • Yasumoto T, Murata M, Satake M, Nagai H (1989) Marine phytoplanktons as a new source of bioactive polyethers. In Miyachi S, Karube I, Ishida Y (eds), Current Topics in Marine Biotechnology. Jpn. Soc. Mar. Biotechnol., Tokyo: 191–194.

    Google Scholar 

  • Yoneda T (1983) Production and preservation of concentratedChlorella and its utilization. Suisan no Kenkyu 2 (5): 52–58. (Japanese)

    Google Scholar 

  • Yoshida Y, Shimizu M, Kawai A, Ishida Y (1995) The unusual occurrence of picophytoplankton with enormous death of ayuPlecoglossus altivelis. Nippon Suisan Gakkaishi 61: 929–949. (Japanese)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Laboratory of Marine Biochemistry, Graduate School of Agricultural Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113, Japan

    Katsumi Yamaguchi

Authors
  1. Katsumi Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yamaguchi, K. Recent advances in microalgal bioscience in Japan, with special reference to utilization of biomass and metabolites: a review. J Appl Phycol 8, 487–502 (1996). https://doi.org/10.1007/BF02186327

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02186327

Key words

Search

Navigation