Abstract
Growth and nitrate uptake were studied on free-living and immobilizedChlorella vulgaris cells cultivated in medium containing different nitrate concentrations. First, the effect of nitrate concentrations on growth indicated that cells can live in the presence of high concentrations as high as 97 mM. Although no lethal effect on cells was observed such concentration a slow down in growth and a decrease in biomass produced was observed. The rate of nitrate uptake increased with the nitrate concentration in the medium. The maximum uptake rate was reached in first days of culture in both free-living and immobilized cells. The rate dropped more rapidly for cells growing in 2 mM nitrate than for cells growing in higher nitrate concentration. The maximum rate was very much the same for free-living and immobilized and was within the order of 0.45 to 0.57 μg NO3 h−1 10−6 cells. Immobilization modified the changes of nitrate uptake rate for concentration higher than 2 mM.
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References
Avissar YJ (1985) Induction of nitrate assimilation in the cyanobacteriumAnabaena variabilis. Physiol. Plant 63: 105–108.
Baudet C, Barbotin JN, Guespin-Michel J (1983) Growth and sporulation ofBacillus subtilis cells. Appl. environ. Microbiol. 45: 297–301.
de la Noüe J, Proulx D (1988) Tertiary treatment of urban waste waters by chitosan-immobilizedPhormidium spp. In Stadler T, Mollion J, Verdus MC, Karamanos W, Morvan H, Christiaen D (eds), Algal Biotechnology Elsevier Applied Science, Barking, 159–168.
Fukui S, Tanaka A (1982) Immobilized microbial cells. Ann. Rev. Microbiol. 36: 145–172.
Flores E, Guerrero MG, Losada M (1983) Photosynthetic nature of nitrate uptake and reduction in the cyanobacteriumAnacystis nidulans. Biochim. Biophys. Acta 722: 408–416.
Flores E, Herrero A, Guerrero MG (1987) Nitrite uptake and its regulation in the cyanobacteriumAnacystis nidulans. Biochim. Biophys. Acta 896: 103–108.
Garbisu C, Hall DO, Serra JL (1992) Nitrate and nitrite uptake by free-living and immobilized N-starved cells ofPhormidium laminosum. J. appl. Phycol. 4: 139–148.
Grizeau D, Navarro JM (1986) Glycerol production byDunaliella tertiolecta immobilized within Ca-alginate beads. Biotechnol. Lett. 8: 261–264.
Guerreo MG, Lara C (1987) Assimilation of inorganic nitrogen. In: Fay P, Van baalen C (eds), The Cyanobacteria, Elsevier Science Publisher B.V. (Biomedical Division) Amsterdam, 163–186.
Herrero A, Flores E, Guerrero MG (1981) Regulation of nitrate reductase levels in the cyanobacteriaAnacystis nidulans, Anabaena sp strain 7119 andNostoc sp strain 6719. J. Bact. 145: 175–180.
Hipkin CR, Syrett PJ (1973) Nitrate reduction by whole cells ofAnkistrodermus braunii andChlamydomonas reinhardi. New Phytol. 72: 47–49.
Jeanfils J (1986) Immobilization of whole cells of green algae or cyanobacteria in insoluble matrices. Morophological observations and nitrite reductase activity of immobilized cells. Arch. Biol. 97: 209–222.
Jeanfils J, Thomas D (1986) Culture and nitrite uptake in immobilizedScenedesmus cells. Appl. Microbiol. Biotechnol. 24: 417–422.
Losada M, Guerrero MG (1979) The photosynthetic reduction of nitrate and its regulation. In: Barber J (ed.), Photosynthesis in Relation to Model System. Elsevier Amsterdam, 365–408.
Mohan RR, Li MN (1975) Nitrate and nitrite reduction by liquid-membrane encapsulated whole cells. Biotechnol. Bioengng 17: 1137–1156.
Oswald WJ (1988) Micro-algae and waste-water treatment. In: Borowitzka MA, Borowitzka LJ (eds), Micro-algal Biotechnology. Cambridge University Press, Cambridge, 305–328.
Pistorius K, Funkhouser E, Voss H (1978) Effect ammonium and ferricyanide on nitrate utilization byChlorella vulgaris. Planta 14: 279–282.
Romero JM, Cornil T, Lara C, Guerrero MG (1987) Modulation of nitrate uptake inAnacystis nidulans by the balance between ammonium assimilation and CO2 fixation. Arch. Biochem. Biophys. 256: 578–584.
Serra J, Llana M, Cadenas E (1978) Nitrate utilization by diatomSkeletonema costatum. Plant. Physiol. 65: 987–990.
Sorokin C, Krauss RW (1958) The effect of light intensity on the growth rates of green algae. Plant Physiol. 33: 109–113.
Terblanche APS (1991) Health hazard of nitrate in drinking water. Water SA 17: 77–82.
Tischner R, Lorenzen H (1979) Nitrate uptake and nitrate reduction in synchronousChlorella. Planta 146: 287–292.
Ulrich WR (1983) Uptake and reduction of nitrate: algae and fungi. Encycl. Plant. Physiol. 15: 376–397.
Vennesland B., Guerrero MG (1979) Reduction of nitrate and nitrite. Encycl. Plant Physiol. 6: 425–434.
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Jeanfils, J., Canisius, MF. & Burlion, N. Effect of high nitrate concentrations on growth and nitrate uptake by free-living and immobilizedChlorella vulgaris cells. J Appl Phycol 5, 369–374 (1993). https://doi.org/10.1007/BF02186240
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DOI: https://doi.org/10.1007/BF02186240