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Characterization of a Photosystem II core and its three-dimensional crystals

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Abstract

A photosystem II core from spinach containing the chlorophyll-binding proteins 47 kDa, 43 kDa, the reaction center proteins D1, D2 and cytochromeb 559 and three low molecular weight polypeptides (MW < 10 kDa) was isolated, its three-dimensional crystals were prepared, and both core and crystals were studied by spectroscopic techniques and electron microscopy. The absorption spectra of the crystallized form of the core indicate a specific orientation of the various pigments within the crystal.

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Abbreviations

EPR:

electron paramagnetic resonance

PS II:

Photosystem II

MES:

4-morpholine-ethanesulfonic acid

DM:

dodecyl-β-D-maltoside

HPLC:

high performance liquid chromatography

PEG:

polyethyleneglycol

RC:

reaction center

References

  • Adir N, Okamura MY and Feher G (1992) Crystallization of the reaction center of Photosystem II. Biophys J 61: A101.

    Google Scholar 

  • Boekema EJ, Dekker JP, van Heel MG, Rögner M, Saerger W, Witt I and Witt HT (1987) Evidence for a trimeric organization of the Photosystem I complex from the thermophilic cyanobacteriumSynechococcus sp. FEBS Lett 217: 283–286.

    Google Scholar 

  • Briantais JM, Vernotte C, Miyao M, Murata N and Picaud M (1985) Relationship between O2 evolution capacity and cytochrome (b 559 ) high-potential form in Photosystem II particles. Biochim Biophys Acta 808: 348–351.

    Google Scholar 

  • Camm ET and Green BR (1989) The chlorophylla/b complex, CP 29, is associated with the Photosystem II reaction center core. Biochim Biophys Acta 974: 180–184.

    Google Scholar 

  • Cramer WA, Theg MS and Widger RW (1986) On the structure and function of cytochromeb 559 . Photosynth Res 10: 393–403.

    Google Scholar 

  • Debus R (1992) The manganese and calcium ions of Photosynthetic oxygen evolution, Biochim Biophys Acta 1102: 269–352.

    Google Scholar 

  • Deisenhofer J, Epp O, Miki K, Huber R and Michel H (1984) X-ray structure analysis of a membrane protein complex. Electron density map at 3 Å resolution and a model of the chromophores of the photosynthetic reaction center fromRhodopseudomonas viridis. J Mol Biol 180: 385–398.

    Google Scholar 

  • Dekker J, Bowlby RN and Yocum CF (1989) Chlorophyll and cytochromeb 559 content of the photochemical reaction center of Photosystem II. FEBS Lett 254: 150–154.

    Google Scholar 

  • De Vitry C, Diner BA and Lemoine Y (1987) Chemical composition of Photosystem II reaction centers (PS II): Phosphorylation of PS II polypeptides. In: Biggins J (ed) Progress in Photosynthesis Research, Vol II, pp 105–108. Martinus Ninhoff, Dordrecht.

    Google Scholar 

  • Fromme P, Boekema EJ and Graber P (1987) Isolation and characterization of a supramolecular complex of subunit III of the ATP-synthase from chloroplasts. Z Naturforsch 42C: 1239–1245.

    Google Scholar 

  • Ghanotakis DF, Demetriou DM and Yocum CF (1987) Isolation and characterization of an oxygen-evolving Photosystem II reaction center core preparation and a 28 kDa Chla-binding protein. Biochim Biophys Acta 891: 15–21.

    Google Scholar 

  • Ghanotakis DF, De Paula JC, Demetriou DM, Bowlby NR, Petersen J, Babcock GT and Yocum CF (1989) Isolation and characterization of the 47 kDa protein and the D1-D2-cytochromeb559 complex. Biochim Biophys Acta 974: 44–53.

    Google Scholar 

  • Ghanotakis DF and Yocum C (1990) Photosystem II and the oxygen-evolving complex. Annu Rev Plant Physiol Plant Mol Biol 41: 255–276.

    Google Scholar 

  • Ikeuchi M, Yuasa M and Inoue Y (1985) Simple and discrete isolation of an O2-evolving PS II reaction center complex retaining Mn and the extrinsic 33 kDa protein. FEBS Lett 185: 316–322.

    Google Scholar 

  • Ikeuchi M and Inue Y (1988) A new 4.8 kDa polypeptide intrinsic to the PS II reaction center, as revealed by modified SDS-PAGE with improved resolution of low-molecularweight proteins. Plant Cell Physiol 29: 1233–1239.

    Google Scholar 

  • Irrgang KD, Boekema EJ, Vater J and Renger G (1988) Structural determination of the Photosystem II core complex from spinach. Eur J Biochem 178: 209–217.

    Google Scholar 

  • Haag E, Irrgang KD, Boekema JE and Renger G (1990) Functional and structural analysis of Photosystem II core complexes from spinach with high oxygen evolution capacity. Eur J Biochem 189: 47–53.

    Google Scholar 

  • Henrysson T, Schroder WP, Spangfort M and Akerlund HE (1989) Isolation and characterization of the chlorophyla/b protein complex CP29 from spinach. Biochim Biophys Acta 977: 301–308.

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.

    Google Scholar 

  • Machold O and Meister A (1979) Resolution of the light-harvesting chlorophylla/b protein ofvicia faba chloroplasts into two different chlorophyll-protein complexes. Biochim Biophys Acta 546: 472–480.

    Google Scholar 

  • McPherson A (1982) Preparation and Analysis of Protein Crystals. Wiley, New York.

    Google Scholar 

  • Miyazaki A, Shina T, Toyoshima Y, Gounaris K and Barber J (1989) Stoichiometry of cytochromeb 559 in Photosystem II. Biochim Biophys Acta 975: 142–147.

    Google Scholar 

  • Murata N, Miyao M, Omata T, Matsunami H and Kuwabara T (1984) Stoichiometry of components in the photosynthetic oxygen evolution system of Photosystem II particles prepared with Triton X-100 from spinach chloroplasts. Biochim Biophys Acta 765: 363–369.

    Google Scholar 

  • Nanba O and Satoh K (1987) Isolation of a Photosystem II reaction centre fraction containing D1 and D2 polypeptides and cytochromeb 559 . Proc Natl Acad Sci USA 84: 109–112.

    Google Scholar 

  • Nakatani HY (1983) Correlation of the low temperature 695 nm fluorescence emission with the reaction centre of PS II (CP47). In: Inoue Y, Crofts AR, Govindjee, Murata N, Renger G, Satoh K (seds) The Oxygen Evolving System of Photosynthesis, pp 49–54. Academic Press, Tokyo.

    Google Scholar 

  • Neville DM Jr (1977) Molecular weight determination of proteindodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem 246: 6328–6334.

    Google Scholar 

  • Rögner M, Dekker JP, Boekema EJ and Witt HT (1987) Size, shape and mass of the oxygen-evolving Photosystem II complex from the thermophilic cyanobacteriumSynechococcus sp. FEBS Lett 219: 207–211.

    Google Scholar 

  • Rutherford AW (1985) Orientation of EPR signals arising from components in Photosystem II membranes. Biochim Biophys Acta 807: 189–201.

    Google Scholar 

  • Rutherford WA (1986) How close is the analogy between the reaction centre of Photosystem II and that of purple bacteria? Biochem Soc Trans 14: 15–17.

    Google Scholar 

  • Sandusky PO, Selvious De Roo CL, Hicks DB, Yocum CF, Ghanotakis DF and Babcock GT (1983) Electron transport activity and polypeptide composition of the isolated Photosystem II complex. In: Inoue Y, Crofts AR, Govindjee, Murata N, Renger G, Satoh K (eds) The Oxygen Evolving Complex of Photosynthesis, pp 189–199. Academic Press, Tokyo.

    Google Scholar 

  • Satoh K (1989) Molecular organization in Photosystem II reaction centre. In: Norris Jr. RJ and Meisel D (eds) Photochemical Energy Conversion, pp 238–250. Elsevier Science Publishing Co, Inc, London.

    Google Scholar 

  • Van Dorssen RJ, Breton J, Plijter JJ, Satoh K, van Gorkom HJ and Amesz J (1987) Spectroscopic properties of the reaction centre and of the 47 kDa chlorophyll protein of Photosystem II Biochim Biophys Acta 893: 267–274.

    Google Scholar 

  • Yamamoto Y, Tabata K, Isogai Y, Nishimura M, Okayama S, Matsuura K and Itoh S (1984) Quantitative analysis of membrane components in a highly active O2-evolving Photosystem II preparation from spinach chloroplasts. Biochim Biophys Acta 765: 363–369.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemistry, University of Crete, Iraklion, Crete, Greece

    Constantina Fotinou & Demetrios F. Ghanotakis

  2. Department Biology, University of Crete, Iraklion, Crete, Greece

    Michael Kokkinidis

  3. Institute of Molecular Biology and Biotechnology, F.O.R.T.H., Iraklion, Crete, Greece

    Constantina Fotinou, Michael Kokkinidis & Demetrios F. Ghanotakis

  4. Max-Planck-Institut für Biophysik, Frankfurt/Main, Germany

    Günter Fritzsch, Winfried Haase & Hartmut Michel

Authors
  1. Constantina Fotinou
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  2. Michael Kokkinidis
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  3. Günter Fritzsch
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  4. Winfried Haase
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  5. Hartmut Michel
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  6. Demetrios F. Ghanotakis
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Fotinou, C., Kokkinidis, M., Fritzsch, G. et al. Characterization of a Photosystem II core and its three-dimensional crystals. Photosynth Res 37, 41–48 (1993). https://doi.org/10.1007/BF02185437

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  • DOI: https://doi.org/10.1007/BF02185437

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