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Modification of symbiotic interaction of pea (Pisum sativum L.) andRhizobium leguminosarum by induced mutations

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Summary

In pea (Pisum sativum L.), mutants could be induced, modified in the symbiotic interaction withRhizobium leguminosarum. Among 250 M2-families, two nodulation resistant mutants (K5 and K9) were obtained. In mutant K5 the nodulation resistance was monogenic recessive and not Rhizobium strain specific. Out of 220 M2-families one mutant nod3 was found which could form nodules at high nitrate concentrations (15 mM KNO3). This mutant nodulated abundantly with severalRhizobium strains, both in the absence and presence of nitrate. Probably as the result of a pleiotropic effect, its root morphology was also changed. Among 1800 M2-families, five nitrate reductase deficient mutants were obtained and one of them (mutant E1) was used to study the inhibitory effect of nitrate on nodulation and nitrogen fixation.

The results of the present investigation show that pea mutants which are modified in their symbiosis withRhizobium leguminosarum, can readily be obtained. The significance of such mutants for fundamental studies of the legume-Rhizobium symbiosis and for applications in plant breeding is discussed.

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References

  1. Blixt S 1972 Mutation genetics inPisum. Agric. Hortique Genetica 30, 1–293.

    Google Scholar 

  2. Blixt S, Marx G A and Murfet J C 1977 Rules for genetic symbols. Pisum Newsl. 9, 67–82.

    Google Scholar 

  3. Braaksma F J 1982 Genetic control of nitrate reduction inArabidopsis thaliana. Ph.D. Thesis. University of Groningen, 109 p.

  4. Feenstra W J and Jacobsen E 1980 Isolation of a nitrate reductase deficient mutant ofPisum sativum by means of selection for chlorate resistance. Theor. Appl. Genet. 58, 39–42.

    Article  Google Scholar 

  5. Feenstra W J, Jacobsen E, van Swaaij A C P M and de Visser A J C 1982 Effect of nitrate on acetylene reduction in a nitrate reductase deficient mutant of pea (Pisum sativum L.). Z. Pflanzenphysiol. 105, 471–474.

    Google Scholar 

  6. Gelin O and Blixt S 1964 Root nodulation in peas. Agri. Hortique Genetica 22, 149–159.

    Google Scholar 

  7. Gibson A H and Pagan J D 1977 Nitrate effects on the nodulation of legumes inoculated with nitrate reductase deficient mutants ofRhizobium. Planta Berlin 134, 17–22.

    Google Scholar 

  8. Hardarson G, Danso S K A and Zapata F 1984 Biological nitrogen fixation in field crops.In Handbook of Plant Science in Agriculture, CRC Press Inc.In press.

  9. Harper J E and Nicolas J C 1978 Nitrogen metabolism of soybeans. Effect of tungstate on nitrate utilization, nodulation and growth. Plant Physiol. 62, 662–664.

    Google Scholar 

  10. Holl F B 1975 Host plant control of the inheritance of dinitrogen fixation in thePisum-Rhizobium symbiosis. Euphytica 24, 767–770.

    Article  Google Scholar 

  11. Jacobsen E and Nijdam H 1983 A mutant showing efficient nodulation in the presence of nitrate. Pisum Newsl. 15, 31–32.

    Google Scholar 

  12. Jacobsen E, Braaksma F J and Feenstra W J 1984 Determination of xanthine dehydrogenase activity in nitrate reductase deficient mutants ofPisum sativum andArabidopsis thaliana. Z. Pflanzenphysiol. 113, 183–188.

    Google Scholar 

  13. Jacobsen E and Feenstra W J 1984 A new pea mutant with efficient nodulation in the presence of nitrate. Plant Sci. Letters 33, 337–344.

    Article  Google Scholar 

  14. Kneen BY E and LaRue T A 1984 EMS derived mutant ofPisum sativum resistant to nodulation.In Advances in Nitrogen Fixation Research. Eds. C Veeger and W E Newton. Martinus Nijhoff/Dr Junk W Publishers and Pudoc Wageningen. 559 p.

    Google Scholar 

  15. Lie T A, Hille Ris Lambers D and Houwers A 1975 Symbiotic specialisation in pea plants: Some environmental effects on nodulation and nitrogen fixation.In Symbiotic Nitrogen Fixation in Plants. Ed P S Nutman. IBP 7, Cambridge University Press, London pp 319–333.

    Google Scholar 

  16. Manhart J R and Wong P P 1980 Nitrate effect on nitrogen fixation (acetylene reduction). Activities of legume root nodules induced by Rhizobia with varied nitrate reductase activities. Plant Physiol. 65, 502–505.

    Google Scholar 

  17. Mil van M 1981 Actual and potential nitrogen fixation in pea and field bean as affected by combined nitrogen. Ph.D. Thesis, Agricultural University, Wageningen., 129 p.

    Google Scholar 

  18. Wong P P 1980 Nitrate and carbohydrate effects on nodulation and nitrogen fixation (acetylene reduction) activity of lentil (Lens esculenta Moench) Plant Physiol. 66, 78–81.

    Google Scholar 

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  1. Department of Genetics, Centre of Biological Sciences, University of Groningen, The Netherlands

    E. Jacobsen

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Jacobsen, E. Modification of symbiotic interaction of pea (Pisum sativum L.) andRhizobium leguminosarum by induced mutations. Plant Soil 82, 427–438 (1984). https://doi.org/10.1007/BF02184280

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