Abstract
Mutations in the chromosomal virulence (chv) region ofA. tumefaciens strain A723 reduce virulence, motility, and ability of the bacteria to bind to plant cells. We conducted experiments to assess the ability ofchv mutants to colonize the rhizosphere ofPisum sativum. The mutation had no effect on ability of bacteria to grow with a defined number of root cap cells as the sole carbon and nitrogen source. Ten days after inoculation, there were up to 103-fold more wild type thanchv mutant bacteria present in the rhizosphere of inoculated plants.
This is a preview of subscription content, log in via an institution to check access.
References
Bhuvanesvari T V et al. 1977 Plant Physiol. 60, 486–491.
Bowen G D and Rovira A D 1976 Annu. Rev. Phytopathol. 14, 121–144.
Bradley D A et al. 1984 Can. J. Microbiol. 30, 676–681.
Brisbane P A and Kerr A 1983 J. Appl. Bacteriol. 54, 425–431.
Curl E A and Truelove B 1986 The Rhizosphere. Springer-Verlag, New York.
Douglas C J et al. 1985 J. Bacteriol. 161, 850–860.
Hawes M C and Pueppke S G 1986 Am. J. Bot. 73, 1466–1473.
Hawes M C and Pueppke S G 1987 Plant Cell Reports 6, 287–290.
Hawes M C et al. 1988 Molecular Plant Microbe Interactions 1, 182–186.
James D W et al. 1985 Appl. Environ. Microbiol. 50, 392–397.
Scher F M et al. 1984 Can. J. Microbiol. 30, 151–157.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hawes, M.C., Pueppke, S.G. Reduced rhizosphere colonization ability ofAgrobacterium tumefaciens chromosomal virulence (chv) mutants. Plant Soil 113, 129–132 (1989). https://doi.org/10.1007/BF02181931
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF02181931