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Effects of nutrient and light limitation on the biochemical composition of phytoplankton

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Abstract

Three marine phytoplankters (Isochrysis galbana, Chaetoceros calcitrans andThalassiosira pseudonana), commonly used in the culture of bivalve larvae, were grown in batch or semi-continuous cultures. Changes in protein, carbohydrate, lipid and some fatty acids were measured as growth became limited by nitrogen, silicon, phosphorus or light. Under N starvation (2 d) the % lipid remained relatively constant, while% carbohydrate increased and% protein decreased in all 3 species compared to cells growing under no nutrient limitation. Under Si starvation (6 h) there was no change in lipid, protein or carbohydrates. The amount of two fatty acids, 20 : 5ω3 and 22 : 6ω3 remained relatively constant under N, P and Si starvation, exept for a sharp drop in the cells of P-starvedT. pseudonana. However, there were pronounced species differences withI. galbana containing significantly less 20 : 5 ω3 thanC. calcitrans orT. pseudonana. Under light limitation the amount of lipid per cell showed no consistent trend over a range of irradiances for all 3 species. The amount of N per cell (an index of protein content) as a function of irradiance, was relatively constant forI. galbana andT. pseudonana, while the amount of N per cell was lower under low irradiances forC. calcitrans. These examples of changes in protein, carbohydrate, lipid and certain fatty acids under nutrient (N, Si or P) or light limitation, emphasize the importance of knowing the phase (e.g. logarithmic vs stationary) of the growth curve in batch cultures, since the nutritional value of the phytoplankters could change as cultures become dense and growth is terminated due to nutrient or light limitation.

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References

  • Ackman RG (1986) WCOT (capillary) gas-liquid chromatography. In: Hamilton RJ, Russell JB (eds), Analysis of Oils and Fats. Elsevier Publ., N.Y., 137–206.

    Google Scholar 

  • Ackman RG, Tocher CS, McLachlan J (1968) Marine phytoplankter fatty acids. J. Fish. Res. Bd Canada 25: 1603–1620.

    Google Scholar 

  • Ben-Amotz A, Tornabene TG, Thomas WH (1965) Chemical profile of selected species of microalgae with emphasis on lipids. J. Phycol. 21: 72–81.

    Google Scholar 

  • Brzezinski MA (1985) The Si : C : N ratio of marine diatoms: interspecific variability and the effect of some environmental variables. J. Phycol. 21: 347–357.

    Google Scholar 

  • Calderwood GS (1989) Effects of nutrient limitation on biochemical composition of three microalgae and their consequent food value to oyster larvae,Crassostrea gigas. M. Sc. thesis, University of British Columbia, Vancouver, B.C. 184 pp.

  • Conley, DJ, Kilman SS, Theriot E (1989) Difference in silica content between marine and freshwater diatoms. Limnol. Oceanogr. 34: 205–213.

    Google Scholar 

  • Conway HL, Harrison PJ (1977) Marine diatoms grown in chemostats under silicate or ammonium limitation. IV. transient response ofChaetoceros debilis, Skeletonema costatum, andThalassiosira gravida to a single addition of the limiting nutrient. Mar. Biol. 43: 33–43.

    Google Scholar 

  • Droop MR (1975) The nutrient status of algal cells in batch culture. J. Mar. Biol. Ass. U.K. 55: 541–555.

    Google Scholar 

  • Elgavish A, Elgavish GA, Halmann M (1980) Phosphorus utilization and storage in batch cultures of the dinoflagellatePeridinium cinctum F. westii. J. Phycol. 16: 626–633.

    Google Scholar 

  • Enright CT, Newkirk GF, Craigie JS, Castell JD (1986) Growth of juvenileOstrea edulis L. fedChaetoceros gracilis Schutt of varied chemical composition. J. Exp. Mar. Biol. Ecol. 96: 15–26.

    Google Scholar 

  • Eppley RW, Holmes RW, Strickland JDH (1967) Sinking rates of marine phytoplankton measured with a fluorometer. J. Exp. Mar. Biol. Ecol. 1: 191–208.

    Google Scholar 

  • Eppley RW, Renger EH (1974) Nitrogen assimilation of an oceanic diatom in nitrogen-limited culture in a chemostat. J. Phycol. 10: 15–23.

    Google Scholar 

  • Goldman JC (1977) Temperature effects on phytoplankton growth in continuous culture. Limnol. Oceanogr. 22: 932–936.

    Google Scholar 

  • Goldman JC (1979) Temperature effects on steady-state growth, phosphorus uptake, and the chemical composition of a marine photoplankter. Microb. Ecol. 5: 153–166.

    Google Scholar 

  • Goldman JC (1986) On phytoplankton growth rates and particulate C : N : P ratios at low light. Limnol. Oceanogr. 31: 1358–1363.

    Google Scholar 

  • Goldman JC, Glibert PM (1982) Comparative rapid ammonium uptake by four species of marine phytoplankton. Limnol. Oceanogr. 27: 814–827.

    Google Scholar 

  • Goldman JC, McCarthy JJ (1978) Steady state growth and ammonium uptake of a fast-growing marine diatom. Limnol. Oceanogr. 23: 695–703.

    Google Scholar 

  • Goldman JC, Peavy DG (1979) Steady-state growth and chemical composition of the marine chlorophyteDunaliella tertiolecta in nitrogen-limited continuous cultures. Appl. Environ. Microbiol. 38: 894–901.

    Google Scholar 

  • Gotham IJ, Rhee G-Y (1981a) Comparative kinetic studies of nitrate-limited growth and nitrate uptake in phytoplankton in continuous culture. J. Phycol. 17: 309–314.

    Google Scholar 

  • Gotham IJ, Rhee G-Y (1981b) Comparative kinetic studies of nitrate-limited growth and phosphate uptake in phytoplankton in continuous culture. J. Phycol. 17: 257–265.

    Google Scholar 

  • Guillard RRL (1975) Culture of phytoplankton for feeding marine invertebrates. In: Smith WL, Chanley MH (eds), Culture of Marine Invertebrate Animals. Plenum Press, N.Y., 29–60.

    Google Scholar 

  • Harrison PJ, Conway HL, Holmes RW, Davis CO (1977) Marine diatoms grown in chemostats under silicate or ammonium limitation. III. Cellular chemical composition and morphology ofChaetoceros debilis, Skeletonema costatum andThalassiosira gravida. Mar. Biol. 43: 19–31.

    Google Scholar 

  • Harrison PJ, Waters RE, Taylor FJR (1980) A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton. J. Phycol. 16: 25–38.

    Google Scholar 

  • Kilham SS, Kott CL, Tilman D (1977) Phosphate and silicate kinetics for the Lake Michigan diatomDiatoma elongatum. J. Great Lakes Res. 3: 93–99.

    Google Scholar 

  • Kowallik W (1978) Blue light effects on carbohydrate and protein metabolism. In: Senger H (ed.), Blue Light Responses: Phenomena and Occurrence in Plants. Vol. 1. CRC Press, Florida, 8–13.

    Google Scholar 

  • Laws EA, Bannister TT (1980) Nutrient- and light-limited growth ofThalassiosira fluviatilis in continuous culture, with implications for phytoplankton growth in the ocean. Limnol. Oceanogr. 25: 457–473.

    Google Scholar 

  • Marsh JB, Weinstein DB (1966) A simple charring method for the determination of lipids. J. Lipid Res. 7: 574–576.

    PubMed  Google Scholar 

  • McLachlan J (1964) Some considerations of the growth of marine algae in artificial media. Can. J. Microbiol. 10: 769–782.

    PubMed  Google Scholar 

  • McLachlan J (1973) Growth media — marine In: Stein JR (ed.), Handbook of Phycological Methods: Culture Methods and Growth Measurements. Cambridge University Press, London, 25–51.

    Google Scholar 

  • Morel FMM, Rueter JG, Anderson DM, Guillard RRL (1979) Aquil: A chemically defined phytoplankton culture medium for trace metal studies. J. Phycol. 15: 135–141.

    Google Scholar 

  • Mortensen SH, Borsheim KY, Rodriguez Rainuzzo J, Knutsen G (1988) Fatty acid and elemental composition of the marine diatomChaetoceros gracilis Schutt. Effects of silicate deprivation, temperature and light intensity. J. Exp. Mar. Biol. Ecol. 122: 173–185.

    Google Scholar 

  • Paasche E (1973) Silicon and the ecology of marine plankton diatoms. I.Thalassiosira pseudonana (Cyclotella nana) grown in a chemostat with silicate as limiting nutrient. Mar. Biol. 19: 117–126.

    Google Scholar 

  • Parslow JS, Harrison PJ, Thompson PA (1984) Saturated uptake kinetics: transient response of the marine diatomThalassiosira pseudonana to ammonium, nitrate, silicate or phosphate starvation. Mar. Biol. 83: 51–59.

    Google Scholar 

  • Parrish CC, Wangersky PJ (1987) Particulate and dissolved lipid classes in cultures ofPhaeodactylum tricornutum grown in cage culture turbidostats with a range of nitrogen supply rates. Mar. Ecol. Prog. Ser. 35: 119–128.

    Google Scholar 

  • Peterson Holm N, Armstrong DE (1981) Effects of Si: P concentration ratios and nutrient limitation on the cellular composition and morphology ofAsterionella formosa (Bacillariophyceae). J. Phycol. 17: 420–424.

    Google Scholar 

  • Rhee G-Y (1978) Effects of N : P atomic ratios and nitrate limitation on algal growth, cell composition and nitrate uptake. Limnol. Oceanogr. 23: 10–23.

    Google Scholar 

  • Rhee G-Y (1982) Effects of environmental factors and their interactions on phytoplankton growth. Adv. Microb. Ecol. 6: 33–74.

    Google Scholar 

  • Rhee G-Y, Gotham IJ (1980) Optimum N : P ratios and coexistence of planktonic algae. J. Phycol. 16: 186–189.

    Google Scholar 

  • Roessler PG (1988) Effects of silicon deficiency on lipid composition and metabolism in the diatomCyclotella cryptica. J. Phycol. 24: 394–400.

    Google Scholar 

  • Schöne HF, Schöne A (1982) MET 44: A weakly enriched seawater medium for ecological studies on marine plankton algae, and some examples of its application. Bot. Mar. 25: 117–122.

    Google Scholar 

  • Shifrin NS, Chisholm SW (1981) Phytoplankton lipids: interspecific differences and effects of nitrate, silicate and light-dark cycles. J. Phycol. 17: 374–384.

    Google Scholar 

  • Suen Y, Hubbard JS, Holzer G, Tornabene TG (1987) Total lipid production of the green algaeNannochloropsis sp. QII under different nitrogen regimes. J. Phycol. 23: 289–296.

    Google Scholar 

  • Terry KL (1980) Nitrogen and phosphorus requirements ofPavlova lutheri in continuous culture. Bot. Mar. 23: 757–764.

    Google Scholar 

  • Tett P, Heaney SI, Droop MR (1985) The Redfield ratio and phytoplankton growth rate. J. Mar. Biol. Ass. U.K. 65: 487–504.

    Google Scholar 

  • Thomas WH, Seibert DLR, Alden M, Neori A, Eldridge P (1984) Yields photosynthetic efficiencies and proximate composition of dense marine microalgal cultures. III.Isochrysis sp. andMonallantus salina experiments and comparative conclusions. Biomass 5: 299–316.

    Google Scholar 

  • Tilman D, Kilham SS (1976) Phosphate and silicate growth and uptake kinetics of the diatomAsterionella formosa andCyclotella meneghiniana in batch and semicontinuous culture. J. Phycol. 12: 375–383.

    Google Scholar 

  • Thompson PA, Harrison PJ, Whyte JNC (In press) The influence of irradiance on phytoplankton fatty acid composition. J. Phycol.

  • Thompson PA, Levasseur ME, Harrison PJ (1989) Light limited growth of a marine diatom on ammonium or nitrate: What is the advantage for marine phytoplankton? Limnol. Oceanogr. 34: 1014–1024.

    Google Scholar 

  • Vaulot D, Olson RJ, Merkel S, Chisholm SW (1987) Cell-cycle response to nutrient starvation in two phytoplankton species,Thalassiosira weissflogii andHymenomonas carterae. Mar. Biol. 95: 625–630.

    Google Scholar 

  • Volkman JK, Jeffrey SW, Nichols PD, Rodgers GI, Garland CD (1989) Fatty acid and lipid composition of 10 species of microalgae used in mariculture. J. Exp. Mar. Biol. Ecol. 128: 219–240.

    Google Scholar 

  • Vonshak A (1986) Laboratory techniques for the cultivation of microalgae. In: Richmond A (ed.), CRC Handbook of Microalgal Mass Culture. CRC Press Inc., Boca Raton, Florida, 117–145.

    Google Scholar 

  • Werner D (1977) Regulation of metabolism by silicate in diatoms. In: Bend G, Lindquist I (eds), Biochemistry of Silicon and Related Problems. Plenum Press, N.Y., 149–176.

    Google Scholar 

  • Whyte JNC (1988) Fatty acid profiles from direct methanolysis of lipids in tissue of cultured species. Aquaculture 75: 193–203.

    Google Scholar 

  • Wynne D, Rhee G-Y (1986) Effects of light intensity and quality on the relative N and P requirements (the optimum N : P ratio) of marine planktonic algae. J. Plankton Res. 8: 91–103.

    Google Scholar 

  • Yoder JA (1979) Effect of temperature on light-limited growth and chemical composition ofSkeletonema costatum (Bacillariophyceae). J. Phycol. 15: 362–370.

    Google Scholar 

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Authors and Affiliations

  1. Department of Oceanography, University of British Columbia, V6T 1W5, Vancouver, B.C., Canada

    P. J. Harrison, P. A. Thompson & G. S. Calderwood

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  1. P. J. Harrison
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  2. P. A. Thompson
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  3. G. S. Calderwood
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Presented at the XIIIth International Seaweed Symposium, University of British Columbia, Vancouver, Canada, August 1989.

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Harrison, P.J., Thompson, P.A. & Calderwood, G.S. Effects of nutrient and light limitation on the biochemical composition of phytoplankton. J Appl Phycol 2, 45–56 (1990). https://doi.org/10.1007/BF02179768

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  • DOI: https://doi.org/10.1007/BF02179768

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