Abstract
The ability of the human DNA topoisomerase IIα and IIβ isozymes to complement functional defects conferred by conditionaltop2 mutations inSaccharomyces cerevisiae has been investigated. At the restrictive temperature,top2 strains show multiple abnormalities, including an inability to complete mitotic and meiotic division owing to a defect in chromosome segregation, and hyper-recombination within the repetitive rDNA gene cluster. We show that the human topoisomerases IIα and IIβ can each support both vegetative growth and the production of viable spores in atop2-4 mutant at the restrictive temperature. Similarly, both human isozymes can rescue a strain carrying atop2 gene disruption, and suppress hyper-recombination within the rDNA gene cluster. We conclude that the human topoisomerase IIα and IIβ isozymes are functionally interchangeable with yeast topoisomerase II and suggest that any isozyme-specific roles in human cells are likely to be dependent upon factors other than inherent differences in catalytic ability between the α and β isozymes.
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Jensen, S., Redwood, C.S., Jenkins, J.R. et al. Human DNA topoisomerases IIα and IIβ can functionally substitute for yeastTOP2 in chromosome segregation and recombination. Molec. Gen. Genet. 252, 79–86 (1996). https://doi.org/10.1007/BF02173207
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DOI: https://doi.org/10.1007/BF02173207