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Determination of endogenous formaldehyde level in human blood and urine by dimedone-14C radiometric method

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Abstract

Radiometric method was developed for the analysis of endogenous formaldehyde in human blood and urine using dimedone-14C reagent. The method based on that dimedone /5,5-dimethyl-cyclohexane-1, 3-dione/ can very easily condense with formaldehyde and other aldehydes. With the help of this radiometric method a sensitive technique and a more accurate evaluation have been made possible. Applying this method it could be established that the formaldehyde level in the blood varies between 0.4–0.6 μg ml−1 and in urine 2.5–4.0 μg ml−1. The error of determination and the chemical bonds of formaldehyde in the biological fluids are discussed.

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Authors and Affiliations

  1. Institute of Isotopes of The Hungarian Academy of Sciences, XII. Konkoly Thege u., P. O. Box 77, H-1525, Budapest, Hungary

    T. Szarvas & J. Volford

  2. II. Surgical Clinic, Postgraduate Medical School, XIII. Szabolcs u. 33-35, Budapest, Hungary

    E. Szatlóczky

  3. Department of Organic Chemical Technology, Technical University of Budapest, Gellért tér 4, H-1111, Budapest, Hungary

    L. Trézl & I. Rusznák

  4. Research Institute of Plant Protection of the Hungarian Academy of Sciences, II. Herman O. u., Budapest, Hungary

    E. Tyihák

Authors
  1. T. Szarvas
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  2. E. Szatlóczky
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  3. J. Volford
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  4. L. Trézl
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  5. E. Tyihák
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  6. I. Rusznák
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Szarvas, T., Szatlóczky, E., Volford, J. et al. Determination of endogenous formaldehyde level in human blood and urine by dimedone-14C radiometric method. Journal of Radioanalytical and Nuclear Chemistry Letters 106, 357–367 (1986). https://doi.org/10.1007/BF02163668

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  • DOI: https://doi.org/10.1007/BF02163668

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