Abstract
A comparison was made between rabbit's eyes which had an intravitreal injection of either 400,000 autologous or homologous skin fibroblasts. The follow-up period was up to 6 months with autologous and 10 weeks with homologous cells. The evaluation involved the clinical assessment of membrane formation, retinal detachment and anterior segment inflammation. In addition, a detailed histological, electron microscopic and autoradiographic study was made of membrane development and changes in the surrounding tissue. Clinical evaluations revealed little difference between the two models. However, on the basis of macroscopic and microscopic examination, homologous cells produced more extensive detachments and more pronounced retinal gliosis. Plasma cells were evident in the choroid 6 weeks after homologous fibroblast injection, but they were not associated to any appreciable extent with the autologous model. Fibrovascular proliferation from around the optic nerve head made a major contribution to the formation of membranes in the experiments with homologous fibroblasts but not those using the autologous cells.
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References
Algvere P, Kock E (1976) Experimental fibroplasia in the rabbit vitreous. Retinal detachment induced by autologous fibroblasts. Graefe's Arch Clin Exp Ophthalmol 199:215–222
Algvere P, Kock E (1983) Experimental epiretinal membranes induced by intravitreal carbon particles. Am J Ophthalmol 96:345–353
Burke JM, Smith JM (1981) Retinal proliferation in response to vitreous hemoglobin or iron. Invest Ophthalmol 20:582–592
Ehrenberg M, Thresher RJ, Machemer R (1984) Vitreous hemorrhage nontoxic to retina as a stimulator of glial and fibrous proliferation. Am J Ophthalmol 97:611–626
Fastenberg DM, Diddie KR, Dorey K, Ryan SJ (1982a) The role of cellular proliferation in an experimental model of massive periretinal proliferation. Am J Ophthalmol 93: 565–572
Fastenberg DM, Diddie KR, Sorgente N, Ryan SJ (1982b) A comparison of different cellular inocula in an experimental model of massive periretinal proliferation. Am J Ophthalmol 93:559–564
Grierson I, Forrester JV (1980) Vitreous haemorrhage and vitreal membranes. Trans Ophthalmol Soc UK 100:140–150
Grierson I, Rahi AHS (1981) Structural basis of contraction in vitreal fibrous membranes. Br J Ophthalmol 65:737–749
Laqua H, Machemer R (1975) Glial cell proliferation in retinal detachment (massive periretinal proliferation). Am J Ophthalmol 80:602–618
Ohsawa E, Miki T (1982) Scanning electron microscopic observation of glial cells following xenon arc photocoagulation. Graefe's Arch Clin Exp Ophthalmol 218:64–69
Ophir A, Blumenkranz MS, Claflin AJ (1982) Experimental intraocular proliferation and neovascularization. Am J Ophthalmol 94:450–457
Stern WH, Fisher SK, Anderson DH, O'Donnell JJ, Erickson PA, Lewis GP, Guerin CJ, Borgula GA, McDermott MA (1982) Epiretinal membrane formation after vitrectomy. Am J Ophthalmol 93:757–772
Sugita G, Tano Y, Machemer R, Abrams G, Claflin A, Fiorentino G (1980) Intravitreal autotransplantation of fibroblasts. Am J Ophthalmol 89: 121–130
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Hitchins, C.A., Grierson, I. & Hiscott, P.S. The effects of injections of cultured fibroblasts into the rabbit vitreous. Graefe's Arch Clin Exp Ophthalmol 223, 237–249 (1985). https://doi.org/10.1007/BF02153654
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DOI: https://doi.org/10.1007/BF02153654