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Numerical taxonomic analysis of cross-inoculation patterns of legumes and Rhizobium

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Summary

A survey was made of published results of tests of the capacity of Rhizobium derived from one legume genus to nodulate plants from other genera. The data were derived from more than 14,000 separate cross-inoculation trials involving species from 165 genera of legumes. Numerical taxonomic techniques were applied to 113 of the genera for which results of substantial cross-infection tests were available. The data were examined using mean character difference coefficients re-expressed as total and positive-only similarity coefficients. The resulting similarity matrices were clustered by the unweighted pair-group method using arithmetic averages. Eighteen affinity groups were defined at the 70% similarity level. With few exceptions, the physiological and cultural behavior of the rhizobia was consistent within the defined groups. Two broad categories were suggested in the numerical taxonomic analysis, and their validity is discussed in regard to the geographic, physiological and cultural characteristics of the legumes and their Rhizobium microsymbionts. The taxonomic and agronomic value of this approach and the new groupings are discussed.

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References

  1. Allen O N and Allen E K 1939 root-nodule bacteria of some tropical leguminous plants: II. Cross-inoculation tests within the cowpea group. Soil Sci. 47, 63–76.

    Google Scholar 

  2. Allen O N and Allen E K 1981 The Leguminosae. Univ. of Wisconsin Press, Madison, Wisc.

    Google Scholar 

  3. Bushnell O A and Sarles W B 1937 Attempts to differentiate the species of Rhizobium by physiological methods. J. Bacteriol. 33, 81–82.

    Google Scholar 

  4. Capitaine L 1912 Étude Analytique et Phytogéographique du Groupe des Légumineuses. Paul Lechevalier, Paris.

    Google Scholar 

  5. DeLey J and Rassel A 1965 DNA base composition, flagellation and taxonomy of the genus Rhizobium. J. Gen. Microbiol. 41, 85–91.

    PubMed  Google Scholar 

  6. Fred E B, Baldwin I L and McCoy E 1932 Root Nodule Bacteria and Leguminous Plants. Univ. of Wisconsin Press, Madison, Wisc.

    Google Scholar 

  7. Graham P H 1964 The application of computer techniques to the taxonomy of the rootnodule bacteria of legumes. J. Gen. Microbiol. 35, 511–517.

    Google Scholar 

  8. Habish H A and Khairi S M 1968 Nodulation of legumes in the Sudan: cross-inoculation groups and the associated Rhizobium strains. Expt. Agric. 4, 227–234.

    Google Scholar 

  9. Hollis A B, Kloos W E and Elkan G H 1981 DNA∶DNA hybridization studies ofRhizobium japonicum and related Rhizobiaceae. J. Gen. Microbiol. 123, 215–22.

    Google Scholar 

  10. Jarvis B D W 1983 Genetic diversity of Rhizobium strains which nodulateLeucaena leucocephala. Curr. Microbiol. 8, 153–158.

    Article  Google Scholar 

  11. Jarvis B D W, Dick A G and Greenwood R M 1980 Deoxyribonucleic acid homology among strains ofRhizobium trifolii and related species. Intl. J. System Bacteriol. 30, 42–52.

    Google Scholar 

  12. Jarvis B D W, MacLean T A, Robertson I G C and Fanning G R 1977 Phenetic similarity and DNA base sequence homology of root nodule bacteria from New Zealand native legumes and Rhizobium strains from agricultural plants. N.Z.J. Agric. Res. 20, 235–248.

    Google Scholar 

  13. Jarvis B D W, Pankhurst C E and Patel J J 1982Rhizobium loti, a new species of legume root nodule bacteria. Intl. J. System. Bacteriol. 32, 378–380.

    Google Scholar 

  14. Jensen H L 1958 The classification of the rhizobia.In Nutrition of the legumes. Ed. E G Hallsworth. Butterworths Scientific Publications, London, pp 75–86.

    Google Scholar 

  15. Jensen H L 1963 Relations de la plante hote avec les Rhizobium du groupeLotus-Anthyllis. Ann. Inst. Pasteur 105, 232–236.

    Google Scholar 

  16. Johnson M D and Allen O N 1952 Cultural reactions of rhizobia with special reference to strains isolated fromSesbania species. Antonie van Leewenhoek J. Microbiol. Serol. 18, 1–12.

    Google Scholar 

  17. Jordan D C 1982 Transfer ofRhizobium japonicum Buchanan 1980 to Bradyrhizobium gen. nov., a genus of slow-growing, root nodule bacteria from leguminous plants. Intl. J. System. Bacteriol. 32, 136–139.

    Google Scholar 

  18. Keyser H H, Bohlool B B, Hu T S and Weber D F 1982 Fast-growing rhizobia isolated from root nodules of soybean. Science 215, 1631–1632.

    Google Scholar 

  19. Krieg N R Ed. Bergey's Manual of Determinative Bacteriology. Vol. 1, Williams and Wilkins Co., Baltimore, Md.

  20. Lange R T 1961 Nodule bacteria associated with the indigenous Leguminosae of South-Western Australia. J. Gen. Microbiol. 26, 351–359.

    PubMed  Google Scholar 

  21. Lindström K, Jarvis B D W, Lindström P E and Patel J J 1983 DNA homology, phagetyping, and cross-nodulation studies of rhizobia infectingGalega species. Can. J. Microbiol. 29, 781–789.

    Google Scholar 

  22. Mannetje L't 1967 A re-examination of the taxonomy of the genus Rhizobium and related genera using numerical analysis. Antonie van Leeuwenhoek J. Microbiol. Serol. 33, 477–491.

    Article  Google Scholar 

  23. Moffett M L and Colwell R R 1968 Adansonian analysis of the Rhizobiaceae. J. Gen. Microbiol. 51, 245–266.

    PubMed  Google Scholar 

  24. Norris D O 1965 Acid production by Rhizobium. A unifying concept. Plant and Soil 22, 143–166.

    Article  Google Scholar 

  25. Roberts G P, Leps W T, Silver L E and Brill W J 1980 Use of two-dimensional polyacrylamide gel electrophoresis to identify and classify Rhizobium strains. Appl. Environ. Microbiol. 39, 414–422.

    Google Scholar 

  26. Sneath P H and Sokal R R 1973 Numerical Taxonomy: The Principles and Practice of Numerical Classification. W H Freeman, San Francisco.

    Google Scholar 

  27. Trinick M J 1980 Relationships amongst the fast-growing rhizobia ofLablab purpureus, Leucaena leucocephala, Mimosa spp.,Acacia farnesiana andSesbania grandiflora and their affinities with other rhizobial groups. J. Appl. Bacteriol. 49, 39–53.

    Google Scholar 

  28. Trinick M J 1982 Host-Rhizobium associations.In Nitrogen Fixation in Legumes. Ed J M Vincent. Academic Press, New York, pp. 111–122.

    Google Scholar 

  29. Trinick M J and Galbraith J 1980 The Rhizobium requirements of the non-legume Parasponia in relationship to the cross-inoculation group concept of legumes. New Phytol. 86, 17–26.

    Google Scholar 

  30. Tutin T G 1958 Classification of the legumes.In Nutrition of the Legumes. Ed E G Hallsworth. Butterworths Scientific Publications, London, pp 3–14.

    Google Scholar 

  31. Vincent J M and Humphrey B 1970 Taxonomically significant group antigens in Rhizobium. J. Gen. Microbiol. 63, 379–382.

    PubMed  Google Scholar 

  32. Wilson J K 1939 Leguminous plants and their associated organisms. Cornell Univ. Agric. Exp. Station Memoir 221, Ithaca, New York.

  33. Zablotowicz R M 1978 Characterization, ecology, and physiology of the cowpea Rhizobium. Ph. D. Thesis, Univ. of California, Riverside, Calif.

    Google Scholar 

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Author notes

  1. M. T. Lieberman

    Present address: Syracuse Research Laboratory, Allied Corp., 13209, Solvay, NY, USA

  2. L. M. Mallory

    Present address: Dept. of Biology, Memphis State University, 38152, Memphis, TN, USA

Authors and Affiliations

  1. Laboratory of Soil Microbiology, Department of Agronomy, Cornell University, 14853, Ithaca, NY, USA

    M. T. Lieberman, L. M. Mallory, Stephen Simkins & Martin Alexander

Authors
  1. M. T. Lieberman
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  2. L. M. Mallory
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  3. Stephen Simkins
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  4. Martin Alexander
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Lieberman, M.T., Mallory, L.M., Simkins, S. et al. Numerical taxonomic analysis of cross-inoculation patterns of legumes and Rhizobium. Plant Soil 84, 225–244 (1985). https://doi.org/10.1007/BF02143186

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  • DOI: https://doi.org/10.1007/BF02143186

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