Über die Verwendung von Myzelsuspensionen als Impfmaterial in Wachstumsversuchen mit Pilzen

Summary

In investigations on fungi incapable of producing spores, conidia, or oidia under laboratory conditions the liquid media used for growth experiments are, according to the ordinary technique, inoculated with mycelium bits cut from colonies of the fungi grown on solid agar media in tubes or Petri dishes. The difficulties and disadvantages met with in employing this method are discussed.

A new method is described using mycelial suspensions for inoculation of liquid substrates. The suspensions are prepared by shaking bits of mycelial mats with glass beads, about 6 mm in diameter, and distilled water or “physiological” salt solution under sterile conditions. The large mycelial particles are allowed to settle. The supernatant suspension is pipetted into a sterile flask and diluted to the propervolume. The suspended particles may be washed by sedimenting in a centrifuge, resuspending in distilled water or salt solution, and recentrifuging under sterile conditions.

Of course the method may be modified in different ways.

The new method is now used on a routine scale in this institute in investigations on mycorrhiza-forming and litter-decomposing fungi.