Abstract
Radioactivity from quinic acid-U-14C was readily incorporated into chlorogenic acid and shikimic acid in healthy andFusarium-infected tomato plants of two varieties, ‘Bonner Beste’ (susceptible) and ‘Moneymaker’ (resistant); radioactively labeled shikimic acid, on the other hand, was converted neither to quinic acid nor to chlorogenic acid.
Infection led to increased incorporation of14C inton-butanol extractives, and alcohol-soluble and insoluble esters, except in the resistant variety after feeding of shikimic acid-U-14C. After infection incorporation into the non-hydrolyzable fraction—which a.o. contains lignin—decreased in the susceptible variety, but it increased in the resistant variety, particularly after administration of shikimic acid-U-14C.
Samenvatting
Radioactief kinazuur werd in gezonde en metFusarium geïnfecteerde tomateplanten behorende tot twee variëteiten, ‘Bonner Beste’ (vatbaar) en ‘Moneymaker’ (resistent), in chlorogeenzuur en shikimizuur omgezet; laatstgenoemde verbinding werd echter noch in kinazuur, noch in chlorogeenzuur omgezet.
Infectie leidde tot een toegenomen incorporatie van14C in metn-butanol extraheerbare verbindingen, en in alcohol-oplosbare en onoplosbare esters, behalve na toediening van radioactief shikimizuur in de resistente variëteit. Incorporatie in de zg. niethydrolyseerbare fractie, die o.a. lignine bevat, bleek in de vatbare variëteit na infectie geringer te zijn, maar in de resistente variëteit na infectie toe te nemen, vooral na toediening van shikimizuur.
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References
Brown, S.A., 1964. Lignin and tannin biosynthesis. In: J. B. Harborne (Editor), Biochemistry of phenolic compounds. Acad. Press, London, New York, pp. 361–398.
Brown, S. A., 1966. Lignins. A. Rev. Pl. Physiol. 17: 223–244.
Chambers, H. L. and Corden, M. E., 1963. Semeiography of Fusarium wilt of tomato. Phytopathology 53: 1006–1010.
Dimond, A. E., 1959. Pathogenesis in the Fusarium wilt diseases. Trans. N.Y. Acad. Sci. II, 21: 609–612.
El-Basyouni, S. Z. and Neish, A. C., 1966. Occurrence of metabolically-active bound forms of cinnamic acid and its phenolic derivatives in acetone powders of wheat and barley plants. Phytochemistry 5: 683–691.
El-Basyouni, S. Z., Neish, A. C. and Towers, G. H. N., 1964. The phenolic acids in wheat. III. Insoluble derivatives of phenolic cinnamic acids as natural intermediates in lignin biosynthesis. Phytochemistry 3: 627–639.
Fuchs, A., Rohringer, R. and Samborski, D. J. 1967. Metabolism of aromatic compounds in healthy and rust-infected primary leaves of wheat. II. Studies with L-phenylalanine-U-14C, L-tyrosine-U-14C, and ferulate-U-14C. Can. J. Bot. 45: 2137–2153.
Gamborg, O. L., 1966a. Aromatic metabolism in plants. II. Enzymes of the shikimate pathway in suspension cultures of plant cells. Can. J. Biochem. 44: 791–799.
Gamborg, O. L., 1966b. Aromatic metabolism in plants. III. Quinate dehydrogenase from mung bean cell suspension cultures. Biochim. biophys. Acta 128: 483–491.
Gamborg, O. L., 1967a. Aromatic metabolism in plants. IV. The interconversion of shikimic acid and quinic acid by enzymes from plant cell cultures. Phytochemistry 6: 1067–1073.
Gamborg, O. L., 1967b. Aromatic metabolism in plants. V. The biosynthesis of chlorogenic acid and lignin in potato cell cultures. Can. J. Biochem. 45: 1451–1457
Hare, R. C., 1964. Indoleacetic acid oxidase. Bot. Rev. 30: 129–165.
Mahin, D. T. and Lofberg, R. T., 1966. A simplified method of sample preparation for determination of tritium, carbon-14, or sulfur-35 in blood or tissue by liquid scintillation counting. Analyt. Biochem. 16: 500–509.
Neish, A.C., Major pathways of biosynthesis of phenols. In: J. B. Harborne (Editor), Biochemistry of phenolic compounds Acad. Press, London, New York, pp. 295–359.
Rohringer, R., Fuchs, A., Lunderstädt, J. and Samborski, D. J., 1967. Metabolism of aromatic compounds in healthy and rust-infected primary leaves of wheat. I. Studies with14CO2, quinate-U-14C, and shikimate-U-14C as precursors. Can. J. Bot. 45: 863–889.
Rohringer, R. and Samborski, D. J., 1967. Aromatic compounds in the host-parasite interaction. A. Rev. Phytopath. 5: 77–86.
Weinstein, L. H., Porter, C. A. and Laurencot, H. J., 1959. Quinic acid as a precursor in aromatic biosynthesis in the rose. Contr. Boyce Thompson Inst. Pl. Res. 20: 121–134.
Weinstein, L. H., Porter, C. A. and Laurencot, H. J., 1961. Role of quinic acid in aromatic biosynthesis in higher plants. Contr. Boyce Thompson Inst. Pl. Res. 21: 201–214.
Weinstein, L. H., Porter, C. A. and Laurencot, H. J., 1962. Role of the shikimic acid pathway in the formation of tryptophan in higher plants: Evidence for an alternative pathway in the bean. Nature, Lond. 194: 205–206.
Zenk, M. H., 1966. Biosynthesis of C6-C1 compounds. In: Biosynthesis of aromatic compounds. Proc. 2nd Meeting F.E.B.S. Pergamon Press, London, 3: 45–60.
Zenk, M. H. und Müller, G., 1964. Biosynthese von p-Hydroxybenzoesäure und anderer Benzoesäurein in höheren Pflanzen. Z. Naturf.19B: 398–405.
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Fuchs, A., de Vries, F.W. Metabolism of radioactively labeled quinic acid and shikimic acid in healthy and Fusarium-infected tomato plants. Netherlands Journal of Plant Pathology 75, 186–192 (1969). https://doi.org/10.1007/BF02137216
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DOI: https://doi.org/10.1007/BF02137216