Skip to main content
SpringerLink
Log in

Silver staining of human aqueous humor proteins resolved by gel electrophoresis

  • Published:
Graefe's Archive for Clinical and Experimental Ophthalmology Aims and scope Submit manuscript

Abstract

The detection of aqueous humor proteins resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was achieved using an ultrasensitive silver staining technique. This procedure permitted the detection of more than 35 different protein bands in human aqueous humor on a one-dimensional gel. Most of the stained proteins had an apparent molecular weight greater than 45,000 daltons.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baba H (1981a) Analysis of aqueous humor protein by microdisc electrophoresis — analysis of aqueous humor protein mainly in glaucoma capsulare. Nippon Ganka Gakkai Zasshi 85(1): 33–36

    Google Scholar 

  • Baba H (1981b) Investigation of aqueous humor proteins by crossed immunoelectrophoresis — analysis of normal human aqueous humor. Nippon Ganka Gakkai Zasshi 85(2): 128–131

    Google Scholar 

  • Dernouchamps JP (1982) The proteins of the aqueous humor. Doc Ophthalmol 53: 193–248

    PubMed  Google Scholar 

  • Hemmingsen L, Øther A (1967) Disc electrophoresis of aqueous humor. Acta Ophthalmol (Copenh) 45: 359–370

    Google Scholar 

  • Herschler JH (1981) The inhibitory factor in aqueous humor. Vision Res 21: 163

    PubMed  Google Scholar 

  • Herschler JH, Claflin AJ, Fiorentino G (1980) The effect of aqueous humor on the growth of subconjunctival fibroblasts in tissue culture and its implications for glaucoma surgery. Am J Ophthalmol 89: 245–249

    PubMed  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685

    PubMed  Google Scholar 

  • Merril CR, Goldman D, Sedman SA, Ebert MH (1981) Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211: 1437–1438

    PubMed  Google Scholar 

  • Merril CR, Goldman D, Van Keuren ML (1982) Simplified silver protein detection and image enhancement methods in polyacrylamide gels. Electrophoresis 3: 17–23

    Google Scholar 

  • Oakley BR, Kirsch DR, Morris NR (1980) A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal Biochem 105: 361–363

    PubMed  Google Scholar 

  • Schmut O, Zirm M (1976) The combination of micro-disk-electrophoresis and electroimmunodiffusion for investigation of aqueous humor proteins. Graefe's Arch Clin Exp Ophthalmol 199(1): 69–74

    Google Scholar 

  • Schmut O, Katschnig H, Zirm M (1977) Investigations of body fluids with low protein content by the combination of microdisc-electrophoresis and electroimmunodiffusion. Z Naturforsch[C] 32(5-6): 405–408

    Google Scholar 

  • Switzer RC, Merril CR, Shifrin S (1979) A highly sensitive silver stain for detecting proteins and peptides in polyacrylamide gels. Anal Biochem 98: 231–237

    PubMed  Google Scholar 

  • von Sallmann L, Moore DH (1948) Electrophoretic patterns of concentrated aqueous humor of rabbit, cattle and horse. Arch Ophthalmol 40: 279–284

    Google Scholar 

  • Zirm M (1980) Proteins in aqueous humor. Adv Ophthalmol 40: 100–172

    PubMed  Google Scholar 

  • Zirm M, Schmut O (1975) Demonstration of proteins in the aqueous humor using combined disc immunoelectrophoresis. Ber Zusammenkunft Dtsch Ophthalmol Ges 73: 426–431

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Ophthalmology, University of Arizona Health Sciences Center, 85724, Tucson, AZ, USA

    B. S. Litin & J. Herschler

Authors
  1. B. S. Litin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Herschler
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Litin, B.S., Herschler, J. Silver staining of human aqueous humor proteins resolved by gel electrophoresis. Graefe's Arch Clin Exp Ophthalmol 221, 290–292 (1984). https://doi.org/10.1007/BF02134126

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02134126

Keywords

Search

Navigation