Abstract
Four hundred and fifty-eight enterotoxigenic strains ofEscherichia coli (ETEC) were examined for the presence of colonisation factor antigens (CFA) I and II, and the putative colonisation factor, E8775, using an immunodiffusion technique with specific antisera. The ETEC strains had been isolated in Thailand, Bangladesh and from travellers returning to Japan from abroad. Approximately 14% of the ETEC strains possessed CFA/I and a further 13% of the strains possessed CFA/II. The E8775 antigen was found on 5% of the strains. CFA/I was found on strains of the serogroups 04, 015, 063, 078, 090, 0110, 0126, 0128, 0153 and 0? CFA/II was found on strains of the serogroups 06, 08, 09, 078, 0115, 0139, 0? and 0 rough. The E8775 antigen was found on strains of the serogroups 025, 0115 and 0167.
The results of this study emphasise the need to continue the search for other mechanisms of adhesion used by ETEC strains, and in particular strains of the serogroups 027, 034, 0148 and 0166.
Similar content being viewed by others
References
Bäck E, Mollby R, Kaijser B, Stintzing G, Wadstrom T, Hable D (1980) EnterotoxigenicEscherichia coli and other gram-negative bacteria of infantile diarrhea: surface antigens, hemagglutinins, colonization factor antigen and loss of enterotoxigenicity. J Infect Dis 142:318–327
Cowan ST, Steel KJ (1965) Manual for the identification of medical bacteria. Cambridge University Press: Cambridge
Cravioto A, Scotland SM, Rowe B (1982) Occurrence of hemagglutination activity and colonization factor antigens I and II in enterotoxigenic and non-enterotoxigenic strainsof Escherichia coli isolated from humans. Infect Immun 36:189–197
Deneke GG, Thorne GM, Gorbach SL (1981) Serotypes of attachment pili of enterotoxigenicEscherichia coli isolated from humans. Infect Immun 32:1254–1260
Evans DG, Silver RP, Evans DJ, Chase DG, Gorbach SL (1975) Plasmid-controlled colonization factor associated with virulence inEscherichia coli enterotoxigenic for humans. Infect Immun 12:656–667
Evans DG, Evans DJ, Tjoa WS, DuPont HL (1978) Detection and characterization of colonization factor of enterotoxigenicEscherichia coli isolated from adults with diarrhea. Infect Immun 19:727–736
Evans DG, Evans DJ (1978) New surface-associated heat-labile colonization factor antigen (CFA/II) produced by enterotoxigenicEscherichia coli of serogroups 06 and 08. Infect Immun 21:638–647
Ørskov F, Ørskov I, Jann B, Jann K (1971) Immunoelectrophoretic patterns of extracts from all O and K antigen test strains. Correlation with pathogenicity. Acta Pathol Microbiol Scan (B) 79:142–152
Ørskov F, Ørskov I (1975)Escherichia coli O∶H serotypes isolated from human blood. Acta Pathol Microbiol Scand (B) 83:595–600
Ørskov I, Ørskov F (1977) Special O∶K∶H serotypes among enterotoxigenicE. coli strains from diarrhoea in adults and children. Med Microbiol Immunol 163:99–110
Ouchterlony O (1949) Antigen-antibody reactions in gels. Acta Pathol Microbiol Scand 26:507–515
Satterwhite TK, Evans DG, DuPont HL, Evans DJ (1978) Role ofEscherichia coli colonisation factor antigen in acute diarrhoea. Lancet II:181–184
Scotland SM, Gross RJ, Rowe B (1977) Serotype related enterotoxigenicity inEscherichia coli 06.H16 and 0148.H28. J Hyg 79:395–403
Thomas LV, Cravioto A, Scotland SM, Rowe B (1982) A new fimbrial antigenic type (E8775) which may represent a colonization factor in enterotoxigenicEscherichia coli in humans. Infect Immun 35:1119–1124
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thomas, L.V., Rowe, B. The occurence of colonisation factors (CFA/I,CFA/II and E8775) in enterotoxigenicEscherichia coli from various countries in South East Asia. Med Microbiol Immunol 171, 85–90 (1982). https://doi.org/10.1007/BF02124915
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02124915