Summary
different enzymes were tested for isolation of intact protoplasts from pollen tetrads. About 80% isolation was achieved from pollen tetrads of Cajanus cajan and Zea mays and about 60% from Luffa cylindrica and Lycopersicon esculentum after 4 h of treatment with 5% cellulase. When these mononucleate protoplasts were incubated in presence of 0.05 M CaCl2 in 0.3 M glucose at pH 10.5, 70–80% fusion was achieved. Fusion was rare in sodium nitrate solutions.
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References
S. S. Bhojwani and E. C. Cocking, Nature New Biol.239, 29 (1972).
P. S. Carlson, Science168, 487 (1970).
G. Melchers and G. Labib, Ber. dt. bot. Ges.83, 129 (1972).
E. C. Cocking, Ann. Rev. Plant physiol.23, 29 (1972).
I. Potrykus, in: Proc. int. symp. yeast, mould and plant protoplasts, Salamanca, p. 319. Academic Press, New York and London 1973.
T. Eriksson, H. Bonnet, K. Glimelius and A. Wallin, in: Tissue Culture and Plant Science, p. 213. Ed. H. E. Street. Academic Press, New York and London 1974.
K. K. Kartha, M. R. Michayluk, K. N. Kao, O. L. Gamborg and F. Constabel, Plant Sci. Lett.3, 265 (1974).
K. N. Kao and M. R. Michayluk, Planta115, 355 (1974).
P. C. Deka and S. K. Sen, Molec. gen. genet.145, 239 (1976).
R. U. Schenk and A. C. Hildebrandt, Can. J. Bot.50, 199 (1972).
W. A. Keller and G. Melchers, Z. Naturforsch.28, 737 (1973).
E. W. Rajasekhar, Nature246, 233 (1973).
M. Ito and M. Media, Exp. Cell Res.80, 453 (1973).
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This work was supported by a grant from the Indian Council of Agricultural Research, New Delhi (No. 1-32/73, Ed. I) in the form of a Senior Fellowship awarded to P. C. D.
Work carried out at Molecular Cytogenetics Research Unit, Dept. of Genetics and Plant Breeding, Banaras Hindu University, Varanasi, India.