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Crossed immunoelectrophoresis and chemical structural analysis used for characterization of two varieties ofEscherichia coli K2 polysaccharide antigen

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Crossed immunoelectrophoresis has shown that theEscherichia coli polysaccharide K2 antigen exists in two forms, K2ab and K2a. In confirmation of this finding, chemical structural analysis showed that K2ab, in addition to the galactose, glycerol and phosphate groups common to K2ab and K2a, contained 0 acetyl groups. The contrast between the earlier and the present K2 designation is discussed and the superiority of the CIE technique for this type of analysis is underlined.

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Authors and Affiliations

  1. Statens Seruminstitut, Collaborative Centre for Reference and Research on Escherichia (WHO), Amager Boulevard 80, DK-2300, Copenhagen, Denmark

    Jens C. Larsen, F. Ørskov & I. Ørskov

  2. Max-Planck-Institut für Immunbiologie, Stübeweg 51, 7800, Freiburg/Zähringen, Federal Republic of Germany

    M. A. Schmidt, B. Jann & K. Jann

Authors
  1. Jens C. Larsen
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  2. F. Ørskov
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  3. I. Ørskov
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  4. M. A. Schmidt
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  5. B. Jann
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  6. K. Jann
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Larsen, J.C., Ørskov, F., Ørskov, I. et al. Crossed immunoelectrophoresis and chemical structural analysis used for characterization of two varieties ofEscherichia coli K2 polysaccharide antigen. Med Microbiol Immunol 168, 191–200 (1980). https://doi.org/10.1007/BF02122853

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  • DOI: https://doi.org/10.1007/BF02122853

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