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Untersuchungen am genetischen Material von Herpesviren

Studies of the genetic material of herpesviruses

II. Genetische Verwandtschaft verschiedener Herpesviren

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Summary

The DNAs of HSV-1 and HSV-2 showed about 65% base-sequence homology as measured by DNA-DNA hybridization experiments. Among the strains of HSV-1 and HSV-2, respectively, existed almost complete homology.The DNA of the Varicella-Zoster virus did not hybridize significantly with that of the herpes simplex viruses. The nucleic acids of ECE-, IBR- and Psr-virus did not share any homology extending the error of the hybridization technique. The specificity of the reaction was demonstrated by reciprocal saturation experiments or by competition hybridization and was proven by the melting of the DNA-DNA hybrid. The homology between HSV-1 and HSV-2 measured by hybridization of transcribed RNA to purified DNA did not exceed 50%. The other viruses did not show significant relatedness in their genetic material. The importance of the experimental results with regard to the biological properties as well as to the evolution of the herpesviruses is discussed.

Zusammenfassung

Die Basensequenz-Homologie für die Nucleinsäuren von HSV-1 und HSV-2 betrug etwa 65%, wie DNS-DNS Hybridisationsexperimente ergaben. Alle Stämme des HSV-1 bzw. HSV-2 waren untereinander in ihrer DNS nahezu vollkommen homolog. Zwischen V-Z-Virus und den Herpes Simplex-Viren bestand keine signifikante Übereinstimmung im genetischen Material. Die Nucleinsäuren von ECE-, IBR-, Psr-Virus wiesen ebenfalls keine Basensequenz-Homologie auf, die über die Fehlerbreite der Methode hinausging. — Die Spezifität der Reaktionen wurde durch reziproke Sättigungshybridisation oder durch Kompetitions-experimente erhärtet und mit dem Aufschmelzen des DNS-DNS Hybridmoleküls nachgewiesen.

Bei HSV-1 und HSV-2 konnte mit der DNS-RNS Hybridbildung in der transkribierten RNS eine Homologie gefunden werden, die 50% nicht überstieg. Die übrigen Viren zeigten auch mit dieser Untersuchungstechnik keine wesentliche Übereinstimmung in ihrem Genmaterial.

Die Bedeutung der experimentellen Befunde für die biologischen Besonderheiten sowie für die Evolution der Herpesviren wird diskutiert.

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Literatur

  1. Wildy, P.: Classification and nomenclature of viruses. Monographs in virology, vol. 5. Basel: Karger 1971.

    Google Scholar 

  2. Biswal, N., Benyesh-Melnick, M.: Complementary nuclear RNAs of murinesarcoma-leukemia virus complex in transformed cells. Proe. nat. Acad. Sci. (Wash.)64, 1372 (1969).

    Google Scholar 

  3. Warnaar, S. O., Cohen, J. A.: A quantitative assay for DNA-DNA hybrids using membrane filters. Biochem. biophys. Res. Commun.24, 554 (1966).

    Article  PubMed  Google Scholar 

  4. Vonka, V., Benyesh-Melnick, M.: Interactions of human cytomegalovirus with human fibroblasts. J. Bact.91, 213 (1966).

    Google Scholar 

  5. Denhardt, D. T.: A membrane-filter technique for the detection of complementary DNA. Biochem. biophys. Res. Commun.23, 641 (1966).

    Article  PubMed  Google Scholar 

  6. Dulbecco, R., Vogt, M.: Plaque formation and isolation of pure lines with poliomyelitis virus. J. exp. Med.99, 167 (1954).

    Article  PubMed  Google Scholar 

  7. Youngner, J. S.: Monolayer tissue culture. I. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells. Proc. Soc. exp. Biol. (N.Y.)85, 202 (1954).

    Google Scholar 

  8. Eagle, H.: Amino acid metabolism in mammalian cell cultures. Science130, 432 (1969).

    Google Scholar 

  9. Hayflick, L., Moorhead, P. S.: The serial cultivation of human diploid cell strains. Exp. Cell Res.25, 585 (1961).

    Article  Google Scholar 

  10. Rapp, F., Benyesh-Melnick, M.: Plaque assay for measurement of cells infected with Zoster virus. Science141, 433 (1963).

    PubMed  Google Scholar 

  11. Benyesh-Melnick, M., Rosenberg, M. S., Watson, B.: Viruses in cell cultures of kidneys of children with congenital heart malformations and other diseases. Proc. Soc. exp. Biol. (N.Y.)117, 452 (1964).

    Google Scholar 

  12. Dreesman, G. R., Benyesh-Melnick, M.: Spectrum of human cytomegalovirus complement-fixing antigens. J. Immunol.99, 1106 (1967).

    PubMed  Google Scholar 

  13. Rowe, W. P., Hartley, J. W., Waterman, S., Turner, H. C., Huebner, R. J.: Cytopathogenic agent resembling human salivary gland virus recovered from tissue cultures of human adenoids. Proc. Soc. exp. Biol. (N.Y.)92, 418 (1956).

    Google Scholar 

  14. Benyesh-Melnick, M., Dessy, S. I., Fernbach, D. J.: Cytomegaloviruria in children with acute leukemia and in other children. Proc. Soc. exp. Biol. (N.Y.)117, 624 (1964).

    Google Scholar 

  15. Weller, T. H., Macauley, J. C., Craig, J. M., Wirth, P.: Isolation of intra-nuclear inclusion producing agents from infants with illnesses resembling cytomegalic inclusion disease. Proc. Soc. exp. Biol. (N.Y.)94, 4 (1957).

    Google Scholar 

  16. McCombs, R., Brunschwig, J. P., Mirkovic, R., Benyesh-Melnick, M.: Electron microscopic characterization of a herpes-like virus isolated from tree shrews. Virology45, 816 (1971).

    Article  PubMed  Google Scholar 

  17. Ludwig, H., Biswal, N., Bryans, J. T., McCombs, R.: Some properties of the DNA from a new equine herpesvirus. Virology45, 534 (1971).

    Article  PubMed  Google Scholar 

  18. Smith, K. O.: Relationship between the envelope and the infectivity of herpes simplex virus. Proc. Soc. exp. Biol. (N.Y.)115, 814 (1964).

    Google Scholar 

  19. Rapp, F.: Variants of herpes simplex virus: isolation, characterization and factors influencing plaque formation. J. Bact.89, 985 (1963).

    Google Scholar 

  20. Rawls, W. E., Laurel, D., Melnick, J. L., Glicksman, J. M., Kaufman, R. H.: A search for viruses in smegma, premalignant and early malignant cervical tissues. The isolation of herpesviruses with distinct antigenic properties. Amer. J. Epidemiol.87, 647 (1968).

    Google Scholar 

  21. —, Iwamoto, K., Adams, E., Melnick, J. L.: Measurement of antibodies to herpesvirus types 1 and 2 in human sera. J. Immunol.104, 599 (1970).

    PubMed  Google Scholar 

  22. Graham, B. J., Ludwig, H., Bronson, D. L., Benyesh-Melnick, M., Biswal, N.: Physicochemical properties of the DNA of herpesviruses. Biochim. biophys. Acta (Amst.)259, 13 (1972).

    Google Scholar 

  23. Madin, S. H., York, C. J., McKercher, D. G.: Isolation of the bovine rhinotracheitis virus. Science124, 721 (1956).

    PubMed  Google Scholar 

  24. Straub, O. C., Del Plab, M. C., Kleine, B.: Infektiöse Rhinotracheitis in einem Milchviehbestand. Tierärztl. Umsch.20, 23 (1965).

    Google Scholar 

  25. Mondino, G., Straub, O. C.: Vergleichende Untersuchungen an zwei europäischen Rhinotracheitisvirusstämmen. Tierärztl. Umsch.2, 569 (1966).

    Google Scholar 

  26. Hughes, J. P., Harvey, J. O., Wada, M.: Keratoconjunctivitis associated with infectious bovine rhinotracheitis. J. Amer. vet. med. Ass.145, 32 (1964).

    PubMed  Google Scholar 

  27. Kendrick, J. W., Gillespie, J. M., McEntee, K.: Infectious pustular vulvo-vaginitis. Cornell Vet.47, 458 (1958).

    Google Scholar 

  28. Straub, O. C., Mäckle, N.: Ein Ausbruch des Bläschenausschlages in einer Besamungsstation. Tierärztl. Umsch.20, 113 (1965).

    Google Scholar 

  29. Steck, F., Raaflaub, W., König, H., Ludwig, H.: Nachweis von IBR-IPV-Virus, Klinik und Pathologie bei zwei Ausbrüchen von Bläschenseuche. Schweiz. Arch. Tierheilk.3, 13 (1969).

    Google Scholar 

  30. Ludwig, H., Paulsen, J., Kaminjolo, J. S.: Kälber-Corneazellen in Kultur. I. Eigenschaften einer fibroblasten- und einer epithelartigen Zellinie und deren Eignung für die Untersuchung einiger tierpathogener Viren. Z. med. Mikrobiol. u. Immunol.155, 133 (1969).

    Google Scholar 

  31. Barenfus, M., Delli Quadri, C. A., McIntyre, R. W., Schroeder, R. J.: Isolation of infectious bovine rhinotracheitis virus from calves with meningoencephalitis. J. Amer. vet. med. Ass.143, 725 (1963).

    PubMed  Google Scholar 

  32. Ludwig, H., Paulsen, J.: Isolierung und fluoreszenzserologische Untersuchungen von Parainfluenza-3-Viren aus Vaginal- und Nasentupfern vom Rind. Zbl. Vet.-Med. B16, 231 (1969).

    Google Scholar 

  33. Ben-Porat, T., Kaplan, A. S.: The chemical composition of herpes simplex and pseudorabies virus. Virology16, 261 (1962).

    Article  PubMed  Google Scholar 

  34. Smith, K. O., Melnick, J. L.: A method for staining virus particles and identifying their nucleic acid type in the electron microscope. Virology17, 480 (1962).

    Article  PubMed  Google Scholar 

  35. McCombs, R. M., Benyesh-Melnick, M., Brunschwig, J. P.: Biophysical studies of vesicular stomatitis virus. J. Bact.91, 803 (1966).

    Google Scholar 

  36. —, Benyesh-Melnick, M., Brunschwig, J. P.: The use of millipore filters in ultrastructural studies of cell cultures and viruses. J. Cell Biol.36, 231 (1968).

    Article  Google Scholar 

  37. Solymosy, F., Fedorsák, I., Gulyás, A., Farkas, G. L., Ehrenberg, L.: A new method based on the use of diethylpyrocarbonate as a nuclease inhibitor for the extraction of undegraded nucleic acid from plant tissues. Europ. J. Biochem.5, 520 (1968).

    Article  PubMed  Google Scholar 

  38. Flamm, W. G., Bond, H. E., Burr, H. E.: Density-gradient centrifugation of DNA in a fixed-angle rotor. A higher order of resolution. Biochim. biophys. Acta (Amst.)129, 310 (1966).

    Google Scholar 

  39. Biswal, N., Kleinschmidt, A. K., Spatz, H. C., Trautner, T. A.: Physical properties of the DNA of bacteriophage SP 50. Molec. Gen. Genetics100, 39 (1967).

    Article  Google Scholar 

  40. Schildkraut, C. L., Maio, J. J.: Studies on the intranuclear distribution and properties of mouse satellite DNA. Biochim. biophys. Acta (Amst.)161, 76 (1968).

    Google Scholar 

  41. Ludwig, H., Biswal, N., Benyesh-Melnick, M.: Characterization of DNA isolated from metaphase chromosomes of cells containing Epstein-Barr virus. Biochim. biophys. Acta (Amst.)232, 261 (1971).

    Google Scholar 

  42. Ifft, J.B., Voet, D.H., Vinograd, J.: The determination of density distributions and density gradients in binary solutions at equilibrium in the ultracentrifuge. J. phys. Chem.65, 1138 (1961).

    Google Scholar 

  43. Schildkraut, C. L., Marmur, J., Doty, P.: Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J. molec. Biol.4, 430 (1962).

    PubMed  Google Scholar 

  44. Vinograd, J., Bruner, R., Kent, R., Weigle, J.: Band-centrifugation of macro-molecules and viruses in self-generating density gradients. Proc. nat. Acad. Sci. (Wash.)49, 902 (1963).

    Google Scholar 

  45. Studier, F. W.: Sedimentation studies of the size and shape of DNA. J. molec. Biol.11, 373 (1965).

    PubMed  Google Scholar 

  46. Freifelder, D.: Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA bacteriophages T4, T5 and T7 and the general problem of determination of M. J. molec. Biol.54, 567 (1970).

    Article  PubMed  Google Scholar 

  47. Mandel, M., Igambi, L., Bergendahl, J., Dodson, M. L., Jr., Scheltgen, E.: Correlation of melting temperature and cesium chloride buoyant density of bacterial deoxyribonucleic acid. J. Bact.101, 333 (1970).

    Article  PubMed  Google Scholar 

  48. Horvath, C. G., Preis, B. A., Lipsky, S. G.: Fast liquid chromatography: An investigation of operating parameters and the separation of nucleotides on pellicular ion exchanges. Analyt. Chem.39, 1422 (1967).

    Article  Google Scholar 

  49. Gillespie, D., Spiegelman, S.: Quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J. molec. Biol.12, 829 (1965).

    PubMed  Google Scholar 

  50. Richards, O. C.: Hybridization of Euglena gracilis chloroplast and nuclear DNA Proc. nat. Acad. Sci. (Wash.)57, 156 (1967).

    Google Scholar 

  51. McConaughy, B. L., McCarthy, B. J.: Related base sequences in the DNA of simple and complex organisms. V. The specificity of interactions between oligonucleotides and denatured DNA. Biochem. Genet.4, 409 (1970).

    Article  PubMed  Google Scholar 

  52. Weissman, C., Borst, P., Curdon, R. H., Billeter, M. A., O'choa, S.: Replication of viral RNA. III. Doublestranded replicative form of MS 2 phage RNA Proc. nat. Acad. Sci. (Wash.)51, 682 (1964).

    Google Scholar 

  53. Ludwig, H., Biswal, N., Benyesh-Melnick, M.: DNA-DNA hybridization studies with nucleic acids of herpes simplex virus type 1, type 2 and pseudo-rabiea virus. Virology (im Druck).

  54. —, Haines, H. G., Biswal, N., Benyesh-Melnick, M.: The characterization of Varicella-Zoster virus DNA. J. gen. Virol.14, 111 (1972).

    PubMed  Google Scholar 

  55. Pertoft, H.: Density gradient centrifugation of a herpes virus (IBR V) in colloidal silica. Virology41, 368 (1970).

    Article  PubMed  Google Scholar 

  56. Wildy, P., Russel, W. C., Horne, R. W.: The morphology of herpes virus. Virology12, 204 (1960).

    Article  PubMed  Google Scholar 

  57. Almeida, J. P., Howatson, A. F., Williams, M. G.: Morphology of Varicella (chicken pox) virus. Virology16, 353 (1962).

    Article  PubMed  Google Scholar 

  58. Abodeely, R. A., Lawson, L. A., Randall, Ch. C.: Morphology and entry of enveloped and deenveloped equine abortion (herpes) virus. J. Virol.5, 513 (1970).

    PubMed  Google Scholar 

  59. Marmur, J.: A procedure for the isolation of DNA from microorganisms. J. molec. Biol.3, 208 (1961).

    Google Scholar 

  60. Morrison, J. M., Keir, H. M.: A new DNA-exonuclease in cells infected with herpes virus: Partial purification and properties of the enzyme. J. gen. Virol.3, 337 (1968).

    PubMed  Google Scholar 

  61. Solymosy, F., Hüvös, P., Gulyás, A., Kapovits, I., Gaál, Ö, Bagi, G., Farkas, G. L.: Diethyl pyrocarbonate, a new tool in the chemical modification of nucleic acids? Biochim. biophys. Acta (Amst.)238, 406 (1971).

    Google Scholar 

  62. Marmur, J., Doty, P.: Determination of the base composition of DNA from its thermal denaturation temperature. J. molec. Biol.5, 109 (1962).

    PubMed  Google Scholar 

  63. Weller, T. H., Witton, H. M., Bell, E. J.: The etiologio agents of varicella and herpes zoster. Isolation, propagation, and cultural characteristics in vitro. J. exp. Med.108, 843 (1958).

    Article  PubMed  Google Scholar 

  64. Melnick, J. L., Midulla, M., Wimberly, I., Barrera-Oro, J. G., Levy, B. M.: A new member of the herpesvirus group isolated from South American marmosets. J. Immunol.92, 596 (1964).

    PubMed  Google Scholar 

  65. Plummer, G., Goodheart, C. R., Henson, D., Bowling, C. P.: A comparative study of the DNA density and behavior in tissue cultures of fourteen different herpesviruses. Virology38, 134 (1969).

    Article  PubMed  Google Scholar 

  66. Crawford, L. V., Lee, A. J.: The nucleic acid of human cytomegalovirus. Virology23, 105 (1964).

    Article  PubMed  Google Scholar 

  67. Graham, B. J., Minamishima, Y., Dreesman, G. R., Haines, H. G., Benyesh-Melnick, M.: Complement-requiring neutralizing (CRN) antibodies in hyper-immune sera to human cytomegaloviruses. J. Immunol.107, 1618 (1971).

    PubMed  Google Scholar 

  68. Haines, H. G., Von Essen, R., Benyesh-Melnick, M.: Preparation of specific antisera to cytomegaloviruses in goats. Proc. Soc. exp. Biol. (N.Y.)138, 846 (1971).

    Google Scholar 

  69. Schneweis, K. E.: Serologische Untersuchungen zur Typendifferenzierung des Herpesvirus hominis. Z. Immunitätsforsch.124, 24 (1962).

    Google Scholar 

  70. Plummer, G., Waner, J. L., Bowling, C. P.: Comparative studies of type 1 and type 2 “herpes simplex” viruses. Brit. J. exp. Path.49, 202 (1968).

    PubMed  Google Scholar 

  71. Nahmias, A. J., Dowdle, W. R.: Antigenic and biologic differences in herpes-virus hominis. Progr. med. Virol.10, 110 (1968).

    Google Scholar 

  72. Ejercito, P. M., Kieff, E. D., Roizman, B.: Characterization of herpes simplex virus strains differing in their effect on social behavior of infected cells. J. gen. Virol.3, 357 (1968).

    Google Scholar 

  73. Goodheart, C. R., Plummer, G., Waner, J. L.: Density difference of DNA of human herpes simplex viruses, types 1 and 2. Virology35, 473 (1968).

    Article  PubMed  Google Scholar 

  74. Graham, B., Ludwig, H., Bronson, D., Benyesh-Melnick, M., Biswal, N.: Physicochemical properties of the DNA of various herpesviruses. Bact. Proc. 170 (1971).

  75. Kieff, E. D., Bachenheimer, S. L., Roizman, B.: Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J. Virol.8, 125 (1971).

    PubMed  Google Scholar 

  76. Bryans, J. T.: The herpesviruses in disease of the horse. Proc. Amer. Ass. Equine Pract. 119 (1968).

  77. Plummer, G., Bowling, C. P., Goodheart, C. R.: Comparison of four horse herpesviruses. J. Virol.4, 738 (1969).

    PubMed  Google Scholar 

  78. Bryans, J. T., Rogers, P., McCollum, W. H., Ludwig, H.: Isolation and characterization of a herpesvirus from an exanthematous progenital disease of the horse. Bacteriol. Proc. 171 (1971).

  79. Mayr, A., Böhm, H. O., Brill, J., Woyciechowska, S.: Charakterisierung eines Stutenabortvirus aus Polen und Vergleich mit bekannten Rhinopneumonitis-virus-Stämmen des Pferdes. Arch. ges. Virusforsch.17, 216 (1965).

    Article  PubMed  Google Scholar 

  80. Kit, S.: Equilibrium sedimentation in density gradients of DNA preparations from animal tissues. J. Mol. Biol.3, 711 (1961).

    PubMed  Google Scholar 

  81. Coudray, Y., Quetier, F., Guille, E.: New compilation of satellite DNA's. Biochim. Biophys. Acta217, 259 (1970).

    PubMed  Google Scholar 

  82. Gillespie, J. H., McEntee, K., Kendrick, J. W., Wagner, W. C.: Comparison of infectious pustular vulvo-vaginitis virus with infectious bovine rhinotracheitis virus. Cornell Vet.49, 288 (1959).

    PubMed  Google Scholar 

  83. McKercher, D. G.: A comparison of the viruses of infectious bovine rhinotracheitis (IBR), infectious pustular vulvovaginitis (IPV) and rinderpest. Can. J. Comp. Med. Vet. Sci.28, 77 (1964).

    Google Scholar 

  84. Bowling, C. P., Goodheart, C. R., Plummer, G.: Oral and genital bovine herpesviruses. J. Virol.3, 95 (1969).

    PubMed  Google Scholar 

  85. Russel, W. C., Crawford, L. V.: Properties of the nucleic acids from some herpes group viruses. Virology22, 288 (1964).

    Article  PubMed  Google Scholar 

  86. Erikson, R. L., Szybalski, W.: The Cs2SO4 equilibrium density gradient and its application for the study of T-even phage DNA: glucosylation and replication. Virology22, 111 (1964).

    Article  Google Scholar 

  87. Wagner, E. K., Roizman, B., Savage, T., Spear, P. G., Mizell, M., Durr, F. E., Sypowicz, D.: Characterization of the DNA of herpesviruses associated with Lucké adenocarcinoma of the frog and Burkitt lymphoma of man. Virology42, 257 (1970).

    Article  PubMed  Google Scholar 

  88. Lee, L. F., Roizman, B., Spear, P. G., Kieft, E. D., Burmester, B. R., Nazerian, K.: Marek's Disease herpes virus: A cytomegalovirus? Proc. nat. Acad, Sci. (N.Y.)64, 951 (1969).

    Google Scholar 

  89. —, Kieff, E. D., Bachenheimer, S. L., Roizman, B., Spear, P. G., Burmester, B. R., Nazerian, K.: Size and composition of Marek's Disease virus deoxyribonucleic acid. J. Virol.7, 289 (1971).

    PubMed  Google Scholar 

  90. Soehner, R. L., Gentry, G. A., Randell, C. C.: Some physicochemical characteristics of equine abortion virus nucleic acid. Virology26, 394 (1965).

    Article  PubMed  Google Scholar 

  91. Plummer, G., Waterson, A. P.: Equine herpesviruses. Virology19, 412 (1963).

    Article  PubMed  Google Scholar 

  92. Karpas, A.: Characterization of a new herpes-like virus isolate from foal-kidney. Ann. Inst. Pasteur110, 688 (1966).

    Google Scholar 

  93. Hsiung, G. D., Fischman, H. R., Fong, C. K. Y., Green, R. H.: Characterization of a cytomegalo-like virus isolated from spontaneously degenerated equine kidney culture. Proc. Soc. exp. Biol. (N.Y.)130, 80 (1969).

    Google Scholar 

  94. Weinberg, A., Becker, Y.: Studies on EB virus of Burkitt's lymphoblasts. Virology39, 312 (1969).

    Article  PubMed  Google Scholar 

  95. Schulte-Holthausen, H., Zur Hausen, H.: Partial purification of the Epstein-Barr virus and some properties of its DNA. Virology40, 776 (1970).

    Article  PubMed  Google Scholar 

  96. Roizman, B.: The herpesviruses — a biochemical definition of the group. Curr. Top. in Microbiol. and Immunol.49, 1 (1969).

    Google Scholar 

  97. Martin, W. B., Hay, D., Crawford, L. V., LeBouvier, G. L., Crawford, M. E.: Characteristics of bovine mammillitis virus. J. gen. Microbiol.46, 325 (1966).

    Google Scholar 

  98. Kaplan, A. S., Ben-Porat, T.: Mode of replication of pseudorabies virus DNA. Virology23, 90 (1964).

    Article  PubMed  Google Scholar 

  99. Becker, Y., Pym, H., Sarov, I.: Herpes simplex virus DNA. Virology36, 184 (1968).

    Article  PubMed  Google Scholar 

  100. Kaplan, A. S.: Herpes simplex and pseudorabies viruses. Virology Monographs, vol. 5. Berlin-Heidelberg-New York: Springer 1969.

    Google Scholar 

  101. Russel, W. C., Crawford, L. V.: Some characteristics of the deoxyribonucleic acid from herpes simplex virus. Virology21, 353 (1963).

    Article  PubMed  Google Scholar 

  102. Ben-Porat, T., Kaplan, A. S.: The chemical composition of herpes simplex and pseudorabies viruses. Virology16, 261 (1962).

    Article  PubMed  Google Scholar 

  103. Cowie, D. B., Avery, R. J., Champe, S. P.: DNA homology among T-even bacteriophages. Virology45, 30 (1971).

    Article  PubMed  Google Scholar 

  104. Fujinaga, K., Piña, M., Green, M.: The mechanism of viral carcinogenesis by DNA mammalian viruses. VI. A new class of virus-specific RNA molecules in cells transformed by group C human adenoviruses. Proc. nat. Acad. Sci. (Wash.)64, 255 (1969).

    Google Scholar 

  105. Scholtissek, C., Rott, R.: Hybridization studies with influenza virus RNA. Virology39, 400 (1969).

    Article  PubMed  Google Scholar 

  106. Walker, P. M. B., McLaren, A.: Specific duplex formation in vitro of mammalian DNA. J. molec. Biol.12, 394 (1965).

    PubMed  Google Scholar 

  107. Aloni, Y., Winocour, E., Sachs, L., Torten, J.: Hybridization between SV 40 DNA and cellular DNA's. J. molec. Biol.44, 333 (1969).

    Article  PubMed  Google Scholar 

  108. Zur Hausen, H., Schulte-Holthausen, H.: Presence of EB virus nucleic acid homology in a “virus free” line of Burkitt tumor cells. Nature227, 245 (1970).

    Article  Google Scholar 

  109. Jacquemont, B., Richard, M. H., Grange, J.: Failure to detect homology between the DNA of the Shope fibroma virus and the DNA of the sensitive cell. J. gen. Virol.10, 237 (1971).

    PubMed  Google Scholar 

  110. McCarthy, B. J., Bolton, E. T.: An approach to the measurement of genetic relatedness among organisms. Proc. nat. Acad. Sci. (Wash.)50, 156 (1963).

    Google Scholar 

  111. Wagner, E. K., Roizman, B.: Ribonucleic acid synthesis in cells infected with herpes simplex virus I. Patterns of ribonucleic acid synthesis in productively infected cells. J. Virol.4, 36 (1969).

    PubMed  Google Scholar 

  112. Crothers, D. M.: Calculation of melting curves for DNA. Biopolymers6, 1391 (1968).

    Article  PubMed  Google Scholar 

  113. Green, M.: Chemical composition of animal viruses. In: The biochemistry of Viruses. H. B. Levy, ed. New York: Marcel Dekker 1969.

    Google Scholar 

  114. Roizman, B., Keller, J. M., Spear, P. G., Terni, M., Nahmias, A., Dowdle, W.: Variability, structural glycoproteins, and classification of herpes simplex viruses. Nature (Lond.)227, 1253 (1970).

    Article  Google Scholar 

  115. Bronson, D. L., Graham, B. J., Ludwig, H., Benyesh-Melnick, M., Biswal, N.: Studies on the relatedness of herpes-viruses through DNA-RNA hybridization. Biochim. biophys. Acta (Amst.)259, 24 (1972).

    Google Scholar 

  116. Subak-Sharpe, H., Bürk, R. R., Crawford, L. V., Morrison, J. M., Hay, J., Keir, H. M.: An approach to evolutionary relationships of mammalian DNA viruses through analysis of the pattern of nearest neighbor base sequences. Cold Spr. Harb. Symp. quant. Biol.31, 737 (1966).

    Google Scholar 

  117. Knippers, R.: Molekulare Genetik. Stuttgart: G. Thieme 1971.

    Google Scholar 

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  1. Institut für Virologie der Justus Liebig-Universität, Gießen

    Hanns Ludwig

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Der experimentelle Teil der Arbeit wurde am Department of Virology and Epidemiology, Baylor College of Medicine, Houston (Texas), durchgeführt.

Herrn Prof. Dr. J. L. Melnick, Frau Prof. Dr. M. Benyesh-Melnick und Herrn Dr. N. Biswal bin ich für die menschliche und wissenschaftliche Unterstützung während meines Aufenthaltes (1969–1971) am Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas, zu großem Dank verpflichtet.

Für die stete Förderung meiner Arbeiten gilt mein besonderer Dank den Herren Prof. Dr. R. Rott und Prof. Dr. C. Scholtissek.

Die Arbeit wurde aus Mitteln des “Special Virus Cancer Program of the National Cancer Institute, NIH” sowie mit einem Stipendium der Deutschen Forschungsgemeinschaft gefördert.

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Ludwig, H. Untersuchungen am genetischen Material von Herpesviren. Med Microbiol Immunol 157, 212–238 (1972). https://doi.org/10.1007/BF02121162

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