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The proteins of Varicella-Zoster-Virus

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[35S] methionine-labelled Varicella-Zoster-Virus (VZV) was partly purified by sucrose density gradient centrifugation. Enveloped virus, checked by electron microscopy, was analyzed by high resolution polyacrylamide gel electrophoresis. A polypeptide pattern of at least 31 proteins could be identified on the fluorogram. The molecular weight of the proteins, estimated by co-electrophoresis with marker proteins, ranged from 18,000 to 240,000 daltons. Immunoprecipitation using a specific human antiserum showed at least 14 proteins.

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References

  1. Bonner, W.M., Laskey, R.A.: A film detection method for tritium labelled proteins and nucleic acid in polyacrylamide gels. Eur. J. Biochem.46 (83–88) 1974

    PubMed  Google Scholar 

  2. Caunt, A.E., Shaw, D.G.: Neutralization test with Varicella-Zoster virus in tissue culture. J. Hyg. (London)67, (343–352) 1969

    Google Scholar 

  3. Dolyniuk, M., Prichett, R., Kieff, E.: Proteins of Epstein-Barr-Virus I. Analysis of the polypeptides of purified enveloped Epstein-Barr-Virus. J. Virol.10 (2606–2617) 1976

    Google Scholar 

  4. Dolyniuk, M., Wolff, E., Kieff, E.: Proteins of Epstein-Barr-Virus II. Electrophoretic analysis of the polypeptides of the nucleocapsid and the glucosamine- and polysaccharide-containing components of enveloped virus. J. Virol.18 (289–297) 1976

    PubMed  Google Scholar 

  5. Harbour, D.A., Caunt, A.E.: Infection of guinea-pig embryo cells with Varicella-Zoster virus. Arch. Virol.49 (39–47) 1975

    PubMed  Google Scholar 

  6. Heine, J.W., Honess, R.W., Cassai, E., Roizman, B.: Proteins specified by Herpes simplex virus XII. The virion polypeptides of type 1 strain. J. Virol.14 (640–651) 1974

    PubMed  Google Scholar 

  7. Honess, R.W., Roizman, B.: Proteins specified by Herpes simplex virus XI. Identification and relative molar rates of structural and nonstructural herpes virus polypeptides in the infected cell. J. Virol.12 (1347–1365) 1973

    PubMed  Google Scholar 

  8. Horwitz, S.M., Scharff, D.M.: Immunological precipitation of radioactivity labelled viral proteins. In: Fundamental techniques in virology (Eds. K. Habel and N.P. Salzman) New York: Academic Press 1969

    Google Scholar 

  9. Meurisse, E.V.: Laboratory studies on the Varicella-Zoster virus. J. Med. Microbiol.2 (317–325) 1969

    PubMed  Google Scholar 

  10. Rapp, F., Benyesch-Melnick, M.: Plaque assay for measurements of cells infected with zoster virus. Science141 (433–434) 1963

    PubMed  Google Scholar 

  11. Sarov, I., Abady, I.: The morphogenesis of human cytomegalovirus. Isolation and polypeptide characterization of cytomegalo virions and dense bodies. Virology66 (464–473) 1975

    PubMed  Google Scholar 

  12. Schmidt, N.J., Lenette, E.H.: Improved yields of cell-free Varicella-Zoster virus. Infect. Immunol.12 (709–715) 1976

    Google Scholar 

  13. Spear, P.G., Roizman, B.: Proteins specified by Herpes simplex virus. V. Purification and structural proteins of the herpesvirion. J. Virol.9 (143–159) 1972

    PubMed  Google Scholar 

  14. Söltz-Sötz, J.: Virologische und serologische Untersuchungen beim Herpes zoster. Arch. Klin. Exp. Derm.220 (105–128) 1964

    PubMed  Google Scholar 

  15. Stinski, M.F.: Synthesis of proteins and glycoproteins in cells infected with human cytomegalovirus. J. Virol.23 (751–767) 1977

    PubMed  Google Scholar 

  16. Strnad, B.C., Aurelian, L.: Proteins of herpesvirus type 2: I. Virion, nonvirion, and antigenic polypeptides in infected cells. Virology69 (438–452) 1976

    PubMed  Google Scholar 

  17. Weller, T.H.: Serial propagation in vitro of agents producing inclusion bodies derived from varicella and herpes zoster. Proc. Soc. Exp. Biol. (N.Y.)83 (340–346) 1953

    Google Scholar 

  18. Weller, T.H., Witton, H.M., Bell, E.J.: The etiologic agents of varicella and herpes zoster. Isolation, propagation and cultural characteristics in vitro. J. Exp. Med.108 (843–868) 1958

    PubMed  Google Scholar 

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Wolff, M.H. The proteins of Varicella-Zoster-Virus. Med Microbiol Immunol 166, 21–28 (1978). https://doi.org/10.1007/BF02121130

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