Skip to main content
SpringerLink
Log in

Inhibition of EB virus transformation of non-adherent human lymphocytes by co-cultivation with adult fibroblasts

  • Published:
Medical Microbiology and Immunology Aims and scope Submit manuscript

Abstract

Transformation of a special population of non-adherent human lymphocytes by EB virus (EBV) is reversibly inhibited by co-cultivation on adult human fibroblasts. Neither fluid from adult fibroblast cultures nor extracts of fibroblasts inhibited such transformation, and the growth of already transformed lymphocytes was not inhibited on adult fibroblasts. The EBV-associated nuclear antigen (EBNA) was detectable in inhibited cultures, with a maximum level of about 15% at 14 days after which it decreased gradually. Reversal of inhibition at 28 days, by either addition of phytohaemagglutinin or by removal of the lymphocytes from the adult fibroblasts, resulted in a prompt increase in the percentage of EBNA-positive cells and typical lymphoblastoid outgrowth. Transformation of EBV-infected non-adherent lymphocytes could be inhibited by the addition of adult fibroblasts up to 2–4 days after infection. The results indicate that, in the EBV-infection of non-adherent lymphocytes on adult fibroblasts, a block resulting in inhibition of transformation occurs between the production of EBNA and the onset of autonomous proliferation of the infected lymphocytes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Casto, B. C.: Adenovirus transformation of hamster embryo cells. J, Virol.2, 376–383 (1968)

    Google Scholar 

  • Henle, G., Henle, W., Clifford, P., Diehl, V., Kafuko, G. W., Kirya, B. G., Klein, G., Morrow, R. H., Munube, G. M. R., Pike, P., Tukei, P. M., Ziegler, J. L.: Antibodies to Epstein-Barr virus in Burkitt's lymphoma and control groups. J. nat. Cancer Inst.43, 1147–1157 (1969)

    PubMed  Google Scholar 

  • Jondal, M., Klein, G.: Surface markers on human B and T lymphocytes. II. Presence of Epstein-Barr virus receptors on B lymphocytes. J. exp. Med.138, 1365–1378 (1973)

    PubMed  Google Scholar 

  • Katz, D. H., Benacerraf, B.: The regulatory influence of activated T cells on B cell responses to antigen. Advanc. Immunol.15, 1–94 (1972)

    Google Scholar 

  • Moss, D. J., Pope, J. H.: Assay of the infectivity of Epstein-Barr virus by transformation of human leucocytesin vitro. J. gen. Virol.17, 233–236 (1972)

    PubMed  Google Scholar 

  • Moss, D. J., Pope, J. H.: EB virus-associated nuclear antigen production and cell proliferation in adult peripheral blood leukocytes inoculated with the QIMR-WIL strain of EB virus. Int. J. Cancer15, 503–511 (1975)

    PubMed  Google Scholar 

  • Pope, J. H.: Establishment of cell lines from Australian leukaemic patients: presence of a herpes-like virus. Aust. J. exp. Biol. med. Sci.46, 643–645 (1968)

    PubMed  Google Scholar 

  • Pope, J. H.: Cell co-operation involved in transformation of leukocytes by the QIMR-WIL strain of EBV. In: Oncogenesis and Herpesviruses II, G. de-Thé, M. A. Epstein, and H. zur Hausen, eds. Lyon: I.A.R.C. Scient. Publns. no. 11, pp. 385–388 (1975)

  • Pope, J. H., Scott, W., Moss, D. J.: Human lymphoid cell transformation by Epstein-Barr virus. Nature New Biol.246, 140–141 (1973)

    PubMed  Google Scholar 

  • Pope, J. H., Scott, W., Moss, D. J.: Cell relationships in transformation of human leukocytes by Epstein-Barr virus. Int. J. Cancer14, 122–129 (1974)

    PubMed  Google Scholar 

  • Reedman, B. M., Klein, G.: Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int. J. Cancer11, 499–520 (1973)

    PubMed  Google Scholar 

  • Scher, C. D.: SV40-induced DNA synthesis and the fixation of the transformed state. Virology46, 956–957 (1971)

    PubMed  Google Scholar 

  • Schneider, U., zur Hausen, H.: Epstein-Barr virus-induced transformation of human leukocytes after cell fractionation. Int. J. Cancer15, 59–66 (1975)

    PubMed  Google Scholar 

  • Todaro, G. J., Green, H.: Cell growth and the initiation of transformation by SV40. Proc. nat. Acad. Sci. (Wash.)55, 302–308 (1966)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Queensland Institute of Medical Research, Brisbane, Australia

    Denis J. Moss, John H. Pope & Walter Scott

Authors
  1. Denis J. Moss
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John H. Pope
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Walter Scott
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by a grant from the National Health and Medical Research Council, Canberra.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Moss, D.J., Pope, J.H. & Scott, W. Inhibition of EB virus transformation of non-adherent human lymphocytes by co-cultivation with adult fibroblasts. Med Microbiol Immunol 162, 159–167 (1976). https://doi.org/10.1007/BF02120993

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02120993

Keywords

Search

Navigation