Abstract
Studies on the regulation of a major histocompatibility complex (MHC) class II gene,HLA-DQA1, in Ia-positive cells (Raji, a human B-lymphoma cell line) and in isogenic la-negative cells (RJ2.2.5, a mutant of Raji altered at theAIR-1 locus) are reported. As previously found,AIR-1 is required in its entirety for the activity of an enhancer factor, the absence of which abolishes transcription of MHC class II genes. In this paper, we show thatHLA-DQA1 gene expression can be directed by an enhancerless promoter. The fact that this promoter is inactive in the RJ2.2.5 mutant suggests that thetrans-acting element determined by theAIR-1 locus is not only an enhancer factor as previously described, but also acts at the MHC class II promoter level.
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The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number M33765.
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Ombra, M.N., Del Pozzo, G., Perfetto, C. et al. Effect of theAIR-1 locus on the activation of an enhancerlessHLA-DQA1 promoter. Immunogenetics 31, 368–376 (1990). https://doi.org/10.1007/BF02115012
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DOI: https://doi.org/10.1007/BF02115012