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Combined effect of human neutrophils, ceftazidime and granulocyte colony-stimulating factor on killing ofEscherichia coli

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Abstract

The combination effect of subinhibitory and inhibitory concentrations of ceftazidime, granulocyte colony-stimulating factor (G-CSF) and polymorphonuclear leukocytes (PMNL) againstEscherichia coli was investigated. PMNL obtained from healthy volunteers were incubated with different concentrations of G-CSF and ceftazidime for 180 min. The addition of 0.25 × MIC of ceftazidime or 1 × MIC significantly enhanced the bactericidal activity of PMNL. G-CSF at a concentration of 6,000 units/ml led to only a slight improvement in the bactericidal activity of PMNL. The combination of 6,000 units/ml G-CSF and ceftazidime in inhibitory as well as subinhibitory concentrations, however, showed a significant synergistic effect (p<0.01) on the antibacterial activity of PMNL during the entire incubation period. Combinations of G-CSF and antibiotics could therefore be beneficial for infected patients, especially those with impaired cellular host defence.

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Authors and Affiliations

  1. Department of Environmental Medicine and Hospital Epidemiology, University Hospital Freiburg, Hugstetterstr. 55, 79106, Freiburg, Germany

    F. D. Daschner, H. Grundmann, K. Anding & S. Lemmen

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  1. F. D. Daschner
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  2. H. Grundmann
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  3. K. Anding
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  4. S. Lemmen
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Daschner, F.D., Grundmann, H., Anding, K. et al. Combined effect of human neutrophils, ceftazidime and granulocyte colony-stimulating factor on killing ofEscherichia coli . Eur. J. Clin. Microbiol. Infect. Dis. 14, 536–539 (1995). https://doi.org/10.1007/BF02113435

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  • DOI: https://doi.org/10.1007/BF02113435

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