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A rapid fluorimetric screening method for chlortetracycline, oxytetracycline and tetracycline in pig meat and kidney tissues

Ein schnelles fluorimetrisches Verfahren zum Nachweis von Chlortetracyclin, Oxytetracyclin und Tetracyclin im Muskel- und Nierengewebe des Schweines

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Zusammenfassung

Es wird ein schnelles fluorimetrisches Verfahren zum Nachweis von Chlortetracyclin, Oxytetracyclin und Tetracyclin im Muskel- und Nierengewebe des Schweines beschrieben. Die Methode umfaßt eine Extraktion mit Essigester, Reinigung und Konzentration des Extraktes mit aromatischen Sulfonsdure-Kationenaustauscher-Kartuschen und den Nachweis durch Fluorescenzmessung. Sie ermöglicht den Nachweis von 0,05 mg/kg Oxytetracyclin, 0,1 mg/kg Chlortetracyclin oder 0,2 mg/kg Tetracyclin.

Summary

A rapid fluorimetric screening method for chlortetracycline, oxytetracycline and tetracycline in pig meat and kidney tissues is described. After sonication-aided extraction with ethyl acetate, the extract is cleaned and concentrated by means of solid-phase extraction using an aromatic sulphonic acid cation-exchange column. Subsequent screening is carried out by fluorimetry. The detection level is estimated to be about 0.05 mg kg−1 for oxytetracycline, 0.1 mg kg−1 for chlortetracycline and 0.2 mg kg−1 for tetracycline.

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Author notes

  1. Marcel J. B. Mengelers

    Present address: State Institute for Quality Control of Agricultural Products, Wageningen, The Netherlands

Authors and Affiliations

  1. Department of the Science of Food of Animal Origin, Chemistry Section, Faculty of Veterinary Medicine, The University of Utrecht, P.O. Box 80 175, NL-3508 TD, Utrecht, The Netherlands

    Nel Haagsma & Marcel J. B. Mengelers

Authors
  1. Nel Haagsma
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  2. Marcel J. B. Mengelers
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Haagsma, N., Mengelers, M.J.B. A rapid fluorimetric screening method for chlortetracycline, oxytetracycline and tetracycline in pig meat and kidney tissues. Z Lebensm Unters Forch 188, 227–231 (1989). https://doi.org/10.1007/BF02112880

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  • DOI: https://doi.org/10.1007/BF02112880

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