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Differential staining of mitotic chromosomes from inbred lines and hybrids ofZea mays L.

Differentialfärbung mitotischer Chromosomen von Inzuchtlinien und deren Hybriden beiZea mays L.

Дифференциальная окраска митотических хромосом инбридинг линий и их гибридов уZea mays L.

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Summary

The Giemsa banding patterns of four inbred lines (DB1113, DB1116, WIR44 and WIR27) ofZea mays and their hybrids have been compared. The polymorphism of knob heterochromatin of 4 chromosome pairs allowed to distinguish the 4 inbred lines on the basis of their banding patterns, reflected the evolutionary distance of the inbred lines and provided evidence as to the origin of these chromosomes in hybrids.

Sister chromatid differentiation by means of BrdUrd incorporation prior to fluorescence plus Giemsa(FPG)-technique revealed 3.7 SCEs per cell for the hybrid BEKOS 251. Individualization of chromosomes on the basis of their replication patterns or asymmetric bands after application of the same technique was impossible.

Zusammenfassung

Die Giemsabandenmuster der vier Inzuchtlinien des Maises DB1113, DB1116 (convar.vulgaris) und WIR27, WIR44 (convar.dentiformis) sowie der daraus entstandenen Hybriden SU 10, Kappa und BEKOS 251 wurden untereinander verglichen. Polymorphismen des Knob-Heterochromatins auf vier Chromosomenpaaren (I, VI, VII, VIII) erlaubten die Unterscheidung der vier Inzuchtlinien anhand ihrer Bandenmuster, widerspiegelten den jeweiligen Verwandtschaftsgrad zwischen den Inzuchtlinien und ermöglichten für einige Chromosomen der Hybriden den Herkunftsnachweis.

Schwesterchromatidendifferenzierung nach BrdUrd-Einbau und FPG-Färbung ergaben für die Doppelhybride BEKOS 251 3.7 SCEs/Zelle. Die Erfassung von Replikationsmustern oder asymmetrischen Banden zur Charakterisierung individueller Chromosomen war mit dieser Methode nicht möglich.

Краткое содержание

Сравнивают вид полос, окрашенных гимзой, четырёх инбридинг линий кукурузы DB1113, DB1116 (convar.vulgaris) и WIR27, WIR44 (convar.dentiformis), а также полученных от них гибридов SU 10, Kappa и BEKOS 251. Полиформизмы кноб-гетерохроматина на четырёх хромосомных парах (I, VI, VII, VIII) дают возможность различить четыре инбридинг линии по виду полос, стражающих состветствующюю степень родства между инбридинг линиями и облегчающиш определение происхождения для отдельных хромосом гибридов.

Дифференцирование сёстренских хроматид после инкорпорации BrdUrd и окраски с FPG дало для двойных гибридов BEKOS 251 3,7 обменов сёстринских хроматид в одной клетке. С помощью этого метода невозможно было показать репликационную картину или асимметрические полосы для характеристики индивидуальных хромосом.

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  1. Nguyen Thi Khuong

    Present address: Department of Genetics, Institute of Biology, National Center for Scientific Research, Nghia Do-Tu Liem — Hanoi, Vietnam

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  1. Zentralinstitut für Genetik und Kulturpflanzenforschung der Akademie der Wissenschaften der DDR, Corrensstraße 3, 4325, Gatersleben, DDR

    I. Schubert

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  1. Nguyen Thi Khuong
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Thi Khuong, N., Schubert, I. Differential staining of mitotic chromosomes from inbred lines and hybrids ofZea mays L.. Die Kulturpflanze 34, 195–205 (1986). https://doi.org/10.1007/BF02112801

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