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Production of siderophore by coagulase-negative staphylococci and its relation to virulence

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Abstract

The ability to produce siderophore is considered to be a virulence factor for many pathogenic bacteria. To determine if siderophore production by coagulase-negative staphylococci (CNS) was related to virulence, 40 clinical isolates of CNS cultured from peritoneal dialysis fluid were compared with 38 commensal skin isolates. Siderophore activity was detected using the chrome azurol S liquid assay. Using precursor studies,Staphylococcus epidermidis isolates were shown to be more likely to produce the siderophore staphyloferrin A. Production of staphyloferrin B amongst non-Staphylococcus epidermidis species was associated with clinical isolates rather than commensal isolates, and therefore may play a role in pathogenicity.

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References

  1. Pfaller MA, Herwaldt LA Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci. Clinical Microbiology Reviews 1988, 1: 281–299.

    PubMed  Google Scholar 

  2. Kloos WE, Bannerman TL Update on clinical significance of coagulase-negative staphylococci. Clinical Microbiology Reviews 1994, 7: 117–140.

    PubMed  Google Scholar 

  3. Sewell CM, Clarridge JE, Young EJ, Guthrie RK Clinical significance of coagulase-negative staphylococci. Journal of Clinical Microbiology 1982, 16: 236–239.

    PubMed  Google Scholar 

  4. Hussain M, Wilcox MH, White PJ The slime of coagulase-negative staphylococci: biochemistry and relation to adherence. FEMS Microbiology Reviews 1993, 104: 191–208.

    Google Scholar 

  5. Wooldridge KG, Williams PH Iron uptake mechanisms of pathogenic bacteria. FEMS Microbiology Reviews 1993, 12: 325–348.

    PubMed  Google Scholar 

  6. Lindsay JA, Riley TV Staphylococcal iron requirements, siderophore production, and iron-regulated protein expression. Infection and Immunity 1994, 62: 2309–2314.

    PubMed  Google Scholar 

  7. Meiwes J, Fiedler H-P, Haag H, Zähner H, Konetschny-Rapp S, Jung G Isolation and characterization of staphyloferrin A, a compound with siderophore activity fromStaphylococcus hyicus DSM 20459. FEMS Microbiology Letters 1990, 67: 201–206.

    Google Scholar 

  8. Konetschny-Rapp S, Jung G, Meiwes J, Zähner H Staphyloferrin A: a structurally new siderophore from staphylococci. European Journal of Biochemistry 1990, 191: 65–74.

    PubMed  Google Scholar 

  9. Drechsel H, Freund S, Nicholson G, Haag H, Jung O, Zähner H, Jung G Purification and chemical characterization of staphyloferrin B, a hydrophilic siderophore from staphylococci. BioMetals 1993, 6: 185–192.

    PubMed  Google Scholar 

  10. Haag H, Fiedler HP, Meiwes J, Drechsel H, Jung G, Zähner H Isolation and biological characterization of staphyloferrin B, a compound with siderophore activity from staphylococci. FEMS Microbiology Letters 1994, 115: 125–130.

    PubMed  Google Scholar 

  11. Lindsay JA, Riley TV Susceptibility to desferrioxamine: a new test for the identification ofStaphylococcus epidermidis. Journal of Medical Microbiology 1991, 35: 45–48.

    PubMed  Google Scholar 

  12. Lindsay JA, Aravena-Román MA, Riley TV Identification ofStaphylococcus epidermidis andStaphylococcus hominis from blood cultures by testing susceptibility to desferrioxamine. European Journal of Clinical Microbiology & Infectious Diseases 1993, 12: 127–131.

    Google Scholar 

  13. Kloos WE Systematics and the natural history of staphylococci. Part I. Journal of Applied Bacteriology 1990, Supplement: 25–37.

    Google Scholar 

  14. Schwyn B, Neilands JB Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 1987, 160: 47–56.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Microbiology, University of Western Australia, Queen Elizabeth II Medical Centre, 6009, Nedlands, Western Australia, Australia

    J. A. Lindsay & B. J. Mee

  2. Department of Clinical Microbiology, Sir Charles Gairdner Hospital, Queen Elizabeth II Medical Centre, 6009, Nedlands, Western Australia, Australia

    T. V. Riley

Authors
  1. J. A. Lindsay
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  2. T. V. Riley
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  3. B. J. Mee
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Lindsay, J.A., Riley, T.V. & Mee, B.J. Production of siderophore by coagulase-negative staphylococci and its relation to virulence. Eur. J. Clin. Microbiol. Infect. Dis. 13, 1063–1066 (1994). https://doi.org/10.1007/BF02111829

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