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Use of a receiver operating characteristic in the evaluation of two commercial enzyme immunoassays for detection ofHelicobacter pylori infection

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Abstract

Two novel commercial IgG enzyme immunoassay (EIA) systems based on acid-glycine-extracted (Pyloriset IgG EIA, Orion Diagnostica) or fast protein liquid chromatography-purified (Cobas Core Anti-H. pylori EIA, Roche Diagnostic Systems)Helicobacter antigens were evaluated in a prospective study involving 127 patients. All patients underwent upper endoscopy with biopsy, and biopsies were examined for the presence ofHelicobacter pylori by a rapid urease test, microscopy and culture. Of the 71 patients found to be infected withHelicobacter pylori, 69 (97.2 %) and 65 (91.5 %) tested positive with the Cobas Core and Pyloriset test, respectively. A detailed receiver operating characteristic analysis of the two tests showed that the Cobas Core assay was more sensitive and specific at every possible cut-off level; gave a better resolution of individual results, indicating a greater fine-sensitivity; and had no grey zone compared to a large grey zone encompassing 13.4 % of the serum samples tested with the Pyloriset EIA. The Cobas Core assay appears to be a valuable tool for epidemiological purposes as well as for pre-endoscopic screening of dyspeptic patients.

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Authors and Affiliations

  1. Department of Bacteriology, Institute of Microbiology and Immunology, University of Ulm, Steinhövelstraße 9, D-89075, Ulm, Germany

    M. Trautmann & R. Marre

  2. Division of Gastroenterology, Department of Internal Medicine, Klinikum Rudolf Virchow, Berlin, Germany

    M. Moldrzyk & J. Körber

  3. Division of Hematology, Department of Internal Medicine, Klinikum Rudolf Virchow, Berlin, Germany

    T. Held

  4. Institute of Medical Microbiology, Free University of Berlin, Berlin, Germany

    K. Vogt

Authors
  1. M. Trautmann
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  2. M. Moldrzyk
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  3. K. Vogt
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  4. J. Körber
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  5. T. Held
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  6. R. Marre
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Trautmann, M., Moldrzyk, M., Vogt, K. et al. Use of a receiver operating characteristic in the evaluation of two commercial enzyme immunoassays for detection ofHelicobacter pylori infection. Eur. J. Clin. Microbiol. Infect. Dis. 13, 812–819 (1994). https://doi.org/10.1007/BF02111341

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